Abstract：
:New technologies have recently enabled saturation mutagenesis and functional analysis of nearly all possible variants of regulatory elements or proteins of interest in single experiments. Here we discuss the past, present, and future of such multiplexed (functional) assays for variant effects (MAVEs). MAVEs provide detailed insight into sequence-function relationships, and they may prove critical for the prospective clinical interpretation of genetic variants.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Gasperini M,Starita L,Shendure Jdoi
10.1038/nprot.2016.135subject
Has Abstractpub_date
2016-10-01 00:00:00pages
1782-7issue
10eissn
1754-2189issn
1750-2799pii
nprot.2016.135journal_volume
11pub_type
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