Abstract：
:Neurofibromatosis type II (NF2) is a disease that lacks effective therapies. NF2 is characterized by bilateral vestibular schwannomas (VSs) that cause progressive and debilitating hearing loss, leading to social isolation and increased rates of depression. A major limitation in NF2 basic and translational research is the lack of animal models that allow the full spectrum of research into the biology and molecular mechanisms of NF2 tumor progression, as well as the effects on neurological function. In this protocol, we describe how to inject schwannoma cells into the mouse brain cerebellopontine angle (CPA) region. We also describe how to apply state-of-the-art intravital imaging and hearing assessment techniques to study tumor growth and hearing loss. In addition, ataxia, angiogenesis, and tumor-stroma interaction assays can be applied, and the model can be used to test the efficacy of novel therapeutic approaches. By studying the disease from every angle, this model offers the potential to unravel the basic biological underpinnings of NF2 and to develop novel therapeutics to control this devastating disease. Our protocol can be adapted to study other diseases within the CPA, including meningiomas, lipomas, vascular malformations, hemangiomas, epidermoid cysts, cerebellar astrocytomas, and metastatic lesions. The entire surgical procedure takes ~45 min per mouse and allows for subsequent longitudinal imaging, as well as neurological and hearing assessment, for up to 2 months.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Chen J,Landegger LD,Sun Y,Ren J,Maimon N,Wu L,Ng MR,Chen JW,Zhang N,Zhao Y,Gao X,Fujita T,Roberge S,Huang P,Jain RK,Plotkin SR,Stankovic KM,Xu Ldoi
10.1038/s41596-018-0105-7subject
Has Abstractpub_date
2019-02-01 00:00:00pages
541-555issue
2eissn
1754-2189issn
1750-2799pii
10.1038/s41596-018-0105-7journal_volume
14pub_type
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