Abstract:
:We describe a step-by-step protocol for measuring the stable products of the nitric oxide (NO) pathway: nitrite, nitrite plus nitrate and nitrate. This described protocol is easy to apply and is about 50 times more sensitive than the commonly used Griess reaction or commercially available assay kits based on the Griess reaction. It also allows the study of minimal changes in the NO pathway. With this method, it takes about 3 h to analyze the above-mentioned stable products in culture supernatants or in various body fluids, and the method has a sensitive linear range of 0.02-10.0 microM. This restricted linear range suggests that the technique is useful for studying small changes of nitrite and nitrate, rather than for routine diagnostic measurements.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Nussler AK,Glanemann M,Schirmeier A,Liu L,Nüssler NCdoi
10.1038/nprot.2006.341subject
Has Abstractpub_date
2006-01-01 00:00:00pages
2223-6issue
5eissn
1754-2189issn
1750-2799pii
nprot.2006.341journal_volume
1pub_type
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