Abstract：
:Apoptosis is a critical factor in AIDS and other viral illnesses, cerebral and myocardial ischemia, autoimmune and neurodegenerative states, organ and bone marrow transplant rejection, and tumor response to chemotherapy and radiation. Improved methods to identify sites of apoptosis are increasing our understanding of the pathophysiology and treatment of these and numerous other human disorders. Here we describe the most used method for labeling annexin V, a protein with a high affinity for apoptotic cells in vitro, with technetium-99m (99mTc) as a radionuclide imaging agent that can localize and non-invasively quantify apoptosis in vivo when coupled with single-photon emission tomography. In this method, annexin V is first attached to the bifunctional chelator molecule hydrazino nicotinate (HYNIC). Once prepared, HYNIC-annexin V can be labeled with 99mTc, a widely available gamma-radiation-emitting radionuclide, for intravenous injection in as little as 30 min without the need for specialized reagents or equipment.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Blankenberg FG,Vanderheyden JL,Strauss HW,Tait JFdoi
10.1038/nprot.2006.17subject
Has Abstractpub_date
2006-01-01 00:00:00pages
108-10issue
1eissn
1754-2189issn
1750-2799pii
nprot.2006.17journal_volume
1pub_type
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