Abstract：
:Studies on colonic cells in the lamina propria (LP) of mice are important for understanding the cellular and immune responses in the gut, especially in inflammatory bowel diseases (such as morbus crohn and colitis ulcerosa). This protocol details a method to isolate LP cells and characterize freshly isolated cells by quality control experiments to obtain cells that can be used for further investigations. After different steps of digestion of the tissue using collagenase, DNase and dispase, the resulting cells are purified using Percoll gradient. The success of the isolation can be analyzed by cell viability test (Trypan Blue exclusion test) and by flow cytometric analysis to assess apoptosis. Finally, the isolated cells can be used for further investigations like comparative studies of mRNA expression, cell-proliferation assay or protein analysis. This protocol can be completed within 6-7 h.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Weigmann B,Tubbe I,Seidel D,Nicolaev A,Becker C,Neurath MFdoi
10.1038/nprot.2007.315subject
Has Abstractpub_date
2007-01-01 00:00:00pages
2307-11issue
10eissn
1754-2189issn
1750-2799pii
nprot.2007.315journal_volume
2pub_type
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