Abstract：
:In most organisms, one crossover (CO) event inhibits the chances of another nearby event. The term used to describe this phenomenon is 'CO interference'. Here, we describe a protocol for quickly generating large data sets that are amenable to CO interference analysis in the flowering plant, Arabidopsis thaliana. We employ a visual assay that utilizes transgenic marker constructs encoding pollen-expressed fluorescent proteins of three colors in the quartet mutant background. In this genetic background, male meiotic products--the pollen grains--remain physically attached thereby facilitating tetrad analysis. We have developed a library of mapped marker insertions that, when crossed together, create adjacent intervals that can be rapidly and simultaneously screened for COs. This assay system is capable of detecting and differentiating single COs as well as two-, three- and four-strand double COs. We also describe how to analyze the data that are produced by this method. To generate and score a double interval in a wild-type and mutant background using this protocol will take 22-27 weeks.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Berchowitz LE,Copenhaver GPdoi
10.1038/nprot.2007.491subject
Has Abstractpub_date
2008-01-01 00:00:00pages
41-50issue
1eissn
1754-2189issn
1750-2799pii
nprot.2007.491journal_volume
3pub_type
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