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Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.

Abstract：

:The membrane-permeant fluorogenic biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin, respectively. Handling of toxic reagents such as arsenic trichloride is minimized so the synthesis can be carried out in a typical chemistry laboratory, usually taking about 2-3 d. A wide range of other biarsenical reagents and intermediates that also bind to tetracysteine-tagged (CysCysProGlyCysCys) proteins can be prepared similarly using this general procedure.

journal_name

Nat Protoc

journal_title

Nature protocols

authors

Adams SR,Tsien RY

doi

10.1038/nprot.2008.144

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

1527-34

issue

9

eissn

1754-2189

issn

1750-2799

pii

nprot.2008.144

journal_volume

3

pub_type

杂志文章

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