Abstract:
:The mouse embryo hindbrain is a robust and adaptable model for studying sprouting angiogenesis. It permits the spatiotemporal analysis of organ vascularization in normal mice and in mouse strains with genetic mutations that result in late embryonic or perinatal lethality. Unlike postnatal models such as retinal angiogenesis or Matrigel implants, there is no requirement for the breeding of conditional knockout mice. The unique architecture of the hindbrain vasculature allows whole-mount immunolabeling of blood vessels and high-resolution imaging, as well as easy quantification of angiogenic sprouting, network density and vessel caliber. The hindbrain model also permits the visualization of ligand binding to blood vessels in situ and the analysis of blood vessel growth within a natural multicellular microenvironment in which endothelial cells (ECs) interact with non-ECs to refine the 3D organ architecture. The entire procedure, from embryo isolation to imaging and through to results analysis, takes approximately 4 d.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Fantin A,Vieira JM,Plein A,Maden CH,Ruhrberg Cdoi
10.1038/nprot.2013.015subject
Has Abstractpub_date
2013-02-01 00:00:00pages
418-29issue
2eissn
1754-2189issn
1750-2799journal_volume
8pub_type
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