Abstract：
:In this protocol, we describe cryoimmunolabeling methods for the subcellular localization of proteins and certain lipids. The methods start with chemical fixation of cells and tissue in formaldehyde (FA) and/or glutaraldehyde (GA), sometimes supplemented with acrolein. Cell and tissue blocks are then immersed in 2.3 M sucrose before freezing in liquid nitrogen. Thin cryosections, cut in an ultracryotome, can be single- or multiple immunolabeled with differently sized gold particles, contrasted and viewed in an electron microscope. Semi-thin cryosections can be used for immunofluorescence microscopy. We describe the detailed procedures that have been developed and tested in practice in our laboratory during the past decades.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Slot JW,Geuze HJdoi
10.1038/nprot.2007.365subject
Has Abstractpub_date
2007-01-01 00:00:00pages
2480-91issue
10eissn
1754-2189issn
1750-2799pii
nprot.2007.365journal_volume
2pub_type
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