Abstract:
:Unlike transplantation of other solid organs, vascularized mouse lung transplantation has only recently been developed. In this protocol, we describe a detailed method for performing a vascularized and aerated mouse orthotopic lung transplant, which to date represents the most physiological mouse model of lung transplantation. The procedure is divided into two separate portions consisting of donor harvest followed by implantation using the cuff technique for bronchovascular anastomoses. After a training period spanning several months, the procedure can be successfully mastered and, in experienced hands, requires approximately 90 min to perform. After an initial learning curve, perioperative survival is close to 100%. As the donor hematopoietic cells in the transplanted lung are replaced by those of the host over time, thereby creating a 'chimeric lung,' this model represents a novel research tool for the study of transplantation biology as well as other disease processes, such as malignancies.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Krupnick AS,Lin X,Li W,Okazaki M,Lai J,Sugimoto S,Richardson SB,Kornfeld CG,Garbow JR,Patterson GA,Gelman AE,Kreisel Ddoi
10.1038/nprot.2008.218subject
Has Abstractpub_date
2009-01-01 00:00:00pages
86-93issue
1eissn
1754-2189issn
1750-2799pii
nprot.2008.218journal_volume
4pub_type
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