Abstract：
:Traumatic brain injury (TBI) is a major cause of mortality and morbidity. Various attempts have been made to replicate clinical TBI using animal models. The fluid-percussion model (FP) is one of the oldest and most commonly used models of experimentally induced TBI. Both central (CFP) and lateral (LFP) variations of the model have been used. Developed initially for use in larger species, the standard FP device was adapted more than 20 years ago to induce consistent degrees of brain injury in rodents. Recently, we developed a microprocessor-controlled, pneumatically driven instrument, micro-FP (MFP), to address operational concerns associated with the use of the standard FP device in rodents. We have characterized the MFP model with regard to injury severity according to behavioral and histological outcomes. In this protocol, we review the FP models and detail surgical procedures for LFP. The surgery involves tracheal intubation, craniotomy and fixation of Luer fittings, and induction of injury. The surgical procedure can be performed within 45-50 min.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Kabadi SV,Hilton GD,Stoica BA,Zapple DN,Faden AIdoi
10.1038/nprot.2010.112subject
Has Abstractpub_date
2010-09-01 00:00:00pages
1552-63issue
9eissn
1754-2189issn
1750-2799pii
nprot.2010.112journal_volume
5pub_type
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