Abstract:
:The pollen tube is an excellent single-cell model system for studying cellular processes in plant cell biology. This protocol describes a detailed step-by-step procedure with optimized conditions for introducing various fluorescent reporter proteins into lily, tobacco and Arabidopsis pollen grains by means of biolistics for their transient expression and subsequent analysis in germinating pollen tubes. The whole experiment consists of four major stages: coating gold microcarriers with DNA constructs, preparation of pollen grains, transformation of plasmid DNA into pollen grains by particle delivery system and germination of bombarded pollen grains in optimized germination media to obtain pollen tubes for protein trafficking, protein localization, drug treatment and organelle dynamics analysis. This protocol takes about 4-12 h from pollen preparation to protein detection.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Wang H,Jiang Ldoi
10.1038/nprot.2011.309subject
Has Abstractpub_date
2011-04-01 00:00:00pages
419-26issue
4eissn
1754-2189issn
1750-2799pii
nprot.2011.309journal_volume
6pub_type
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