Abstract：
:Large-insert BAC (bacterial artificial chromosome) and BIBAC (binary BAC) libraries are essential for modern genomics research for all organisms. We helped pioneer the BAC and BIBAC technologies, and by using them we have constructed hundreds of BAC and BIBAC libraries for different species of plants, animals, marine animals, insects, algae and microbes. These libraries have been used globally for different aspects of genomics research. Here we describe the procedure with the latest improvements that we have made and used for construction of BIBAC libraries. The procedure includes the preparation of BIBAC vectors, the preparation of clonable fragments of the desired size from the source DNA, the construction and transformation of BIBACs and, finally, the characterization and assembly of BIBAC libraries. We also specify the modifications necessary for construction of BAC libraries using the protocol. The entire protocol takes ∼7 d.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Zhang HB,Scheuring CF,Zhang M,Zhang Y,Wu CC,Dong JJ,Li Ydoi
10.1038/nprot.2011.456subject
Has Abstractpub_date
2012-02-16 00:00:00pages
479-99issue
3eissn
1754-2189issn
1750-2799pii
nprot.2011.456journal_volume
7pub_type
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