Abstract：
:We describe a simple and versatile scheme to prepare a series of poly(ethylene glycol)-based bidentate ligands that permit strong interactions with colloidal semiconductor nanocrystals (quantum dots, QDs) and gold nanoparticles (AuNPs) alike and promote their dispersion in aqueous solutions. These ligands are synthesized by coupling poly(ethylene glycol)s of various chain length to thioctic acid, followed by ring opening of the 1,2-dithiolane moiety to create a bidentate thiol anchoring group with enhanced affinity for CdSe-ZnS core-shell QDs. These ligands provide a straightforward means of preparing QDs and AuNPs that exhibit greater resistance to environmental changes, facilitating their effective use in bioassays and live cell imaging.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Mei BC,Susumu K,Medintz IL,Mattoussi Hdoi
10.1038/nprot.2008.243subject
Has Abstractpub_date
2009-01-01 00:00:00pages
412-23issue
3eissn
1754-2189issn
1750-2799pii
nprot.2008.243journal_volume
4pub_type
杂志文章相关文献
Nature Protocols文献大全abstract：:This protocol represents a novel enzyme-luminescence method to detect dopamine sensitively and rapidly with high temporal resolution. In principle, dopamine is first oxidized with tyramine oxidase to produce H(2)O(2), and then the produced H(2)O(2) reacts with luminol to generate chemiluminescence in the presence of h...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2008.158
更新日期：2008-01-01 00:00:00
abstract：:Here we present a protocol for labeling aliphatic carboxylic acids with the positron-emitting radionuclide 11C (t(1/2) = 20.4 min) at the carboxyl position using [11C]carbon monoxide via photoinitiated free radical-mediated carbonylation. A solution of an alkyl iodide in a homogenous binary organic solvent-water mixtu...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.112
更新日期：2006-01-01 00:00:00
abstract：:This protocol describes how to purify and radiolabel collagen for use as a substrate to assay collagenolytic members of the matrix metalloproteinases (MMPs). This assay measures enzymes that specifically cleave native triple helical collagen. After incubation of the MMP enzyme with the collagen substrate at 37 degrees...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2008.244
更新日期：2009-01-01 00:00:00
abstract：:Determining enantiomeric excess (e.e.) in chiral compounds is key to development of chiral catalyst auxiliaries and chiral drugs. Here we describe a sensitive and robust fluorescence-based assay for determining e.e. in mixtures of enantiomers of 1,2- and 1,3-diols, chiral amines, amino alcohols, and amino-acid esters....
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-020-0329-1
更新日期：2020-07-01 00:00:00
abstract：:The scototaxis (dark/light preference) protocol is a behavioral model for fish that is being validated to assess the antianxiety effects of pharmacological agents and the behavioral effects of toxic substances, and to investigate the (epi)genetic bases of anxiety-related behavior. Briefly, a fish is placed in a centra...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2009.225
更新日期：2010-02-01 00:00:00
abstract：:Single-cell electroporation allows transfection of plasmid DNA or macromolecules into individual living cells using modified patch electrodes and common electrophysiological equipment. This protocol is optimized for rapid in vivo electroporation of Xenopus laevis tadpole brains with DNA, dextrans, morpholinos and comb...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.186
更新日期：2006-01-01 00:00:00
abstract：:The specialized microfold cells (M cells) in the follicle-associated epithelium (FAE) of intestinal Peyer's patches serve as antigen-sampling cells of the intestinal innate immune system. Unlike 'classical' enterocytes, they are able to translocate diverse particulates without digesting them. They act as pathways for ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2017.041
更新日期：2017-07-01 00:00:00
abstract：:The analysis of highly hydrophobic proteins is still an analytical challenge. Using a recombinant gamma-aminobutyric acid A (GABAA)-receptor subunit as a model protein, we developed a gel-based proteomic approach for high MS/MS-peptide sequence coverage identification. Protein samples were separated by multi-dimension...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2009.92
更新日期：2009-01-01 00:00:00
abstract：:The use of anisotropic NMR data, such as residual dipolar couplings (RDCs) and residual chemical shift anisotropies (RCSAs), has emerged as a powerful technique for structural characterization of organic small molecules. RDCs typically report the relative orientations of different 1H-13C bonds; RCSAs report the relati...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-018-0091-9
更新日期：2019-01-01 00:00:00
abstract：:Mass spectrometry-based phosphoproteomic analysis is a powerful method for gaining a global, unbiased understanding of cellular signaling. Its accuracy and comprehensiveness stands or falls with the quality and choice of the applied phosphopeptide prefractionation strategy. This protocol covers a powerful but simple a...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2015.134
更新日期：2016-01-01 00:00:00
abstract：:This protocol describes the efficient, generally applicable Ullmann coupling reaction of bromaminic acid with alkyl- or aryl-amines in phosphate buffer under microwave irradiation using elemental copper as a catalyst. The reaction leads to a number of biologically active compounds. As a prototypical example, the synth...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2010.63
更新日期：2010-05-01 00:00:00
abstract：:In order to meaningfully analyze common and rare genetic variants, results from genome-wide association studies (GWASs) of multiple cohorts need to be combined in a meta-analysis in order to obtain enough power. This requires all cohorts to have the same single-nucleotide polymorphisms (SNPs) in their GWASs. To this e...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2015.077
更新日期：2015-09-01 00:00:00
abstract：:The elucidation of protein-protein interaction networks is a crucial task in the postgenomic era. In this protocol, we describe our approach to discover protein-protein interactions using the surface plasmon resonance technique coupled to mass spectrometry (MS). A peptide or a protein is immobilized on a sensor chip a...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2009.84
更新日期：2009-01-01 00:00:00
abstract：:Pseudo-afferent cervical lymph-duct cannulation in a sheep model allows large amounts of lymph cells to be collected under physiological conditions, carrying immune signaling information from the head tissues, including oro-nasal mucosae. Importantly, large quantities of dendritic cells (DCs) of several subtypes are o...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.147
更新日期：2006-01-01 00:00:00
abstract：:Here, we describe a simple protocol for the design and construction of a laser-guided direct writing (LGDW) system able to micropattern the self-assembly of liver sinusoid-like structures with micrometer resolution in vitro. To the best of our knowledge, LGDW is the only technique able to pattern cells "on the fly" wi...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.386
更新日期：2006-01-01 00:00:00
abstract：:Cell-to-cell chemical signaling plays multiple roles in coordinating the activity of the functional elements of an organism, with these elements ranging from a three-neuron reflex circuit to the entire animal. In recent years, single-cell mass spectrometry (MS) has enabled the discovery of cell-to-cell signaling molec...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.277
更新日期：2007-01-01 00:00:00
abstract：:Blood circulating endothelial cells (CECs) and circulating hematopoietic progenitor cells (CPCs) represent two cell populations that are thought to play important roles in tissue vascularization. CECs and CPCs are currently studied as surrogate markers in patients for more than a dozen pathologies, including heart dis...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.111
更新日期：2007-01-01 00:00:00
abstract：:The direct detection of drug-protein interactions in living cells is a major challenge in drug discovery research. Recently, we introduced an approach termed thermal proteome profiling (TPP), which enables the monitoring of changes in protein thermal stability across the proteome using quantitative mass spectrometry. ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2015.101
更新日期：2015-10-01 00:00:00
abstract：:The transmembrane (TM) anchors of cell surface proteins have been one of the 'blind spots' in structural biology because they are generally very hydrophobic, sometimes dynamic, and thus difficult targets for structural characterization. A plethora of examples show these membrane anchors are not merely anchors but can ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-019-0188-9
更新日期：2019-08-01 00:00:00
abstract：:An important area of proteomics involves the need for quantification, whether relative or absolute. Many methods now exist for relative quantification, but to support biomarker proteomics and systems biology, absolute quantification rather than relative quantification is required. Absolute quantification usually invol...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.129
更新日期：2006-01-01 00:00:00
abstract：:The accurate knowledge of receptor kinetics is crucial to our understanding of cell signal transduction in general and neural function in particular. The classical technique of probing membrane receptors on a millisecond scale involves placing a recording micropipette with a membrane patch in front of a double-barrel ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2013.075
更新日期：2013-01-01 00:00:00
abstract：:Cap-analysis gene expression (CAGE) provides accurate high-throughput measurement of RNA expression. CAGE allows mapping of all the initiation sites of both capped coding and noncoding RNAs. In addition, transcriptional start sites within promoters are characterized at single-nucleotide resolution. The latter allows t...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2012.005
更新日期：2012-02-23 00:00:00
abstract：:This protocol describes a method for the extraction of DNA from elephant ivory. These techniques are being used to assign geographic origin to poached ivory by comparing the ivory genotype to a geographic-based gene frequency map, developed separately. The method has three components: ivory pulverization, decalcificat...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.318
更新日期：2007-01-01 00:00:00
abstract：:Rapid evaluation of microbial cell-surface carbohydrates is essential to understanding the mechanisms by which bacteria use glycans to establish pathogenic or symbiotic relationships. Microbial glycan analysis is complicated both by the vast diversity of possible carbohydrate structures and by their dynamic nature. Ba...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.76
更新日期：2006-01-01 00:00:00
abstract：:We describe a step-by-step protocol for measuring the stable products of the nitric oxide (NO) pathway: nitrite, nitrite plus nitrate and nitrate. This described protocol is easy to apply and is about 50 times more sensitive than the commonly used Griess reaction or commercially available assay kits based on the Gries...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.341
更新日期：2006-01-01 00:00:00
abstract：:A big challenge in proteomics is the identification of cell-type-specific proteomes in vivo. This protocol describes how to label, purify and identify cell-type-specific proteomes in living mice. To make this possible, we created a Cre-recombinase-inducible mouse line expressing a mutant methionyl-tRNA synthetase (L27...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-018-0106-6
更新日期：2019-02-01 00:00:00
abstract：:Here we present a protocol for the extraction of RNA from Dictyostelium discoideum. Dictyostelium is a social amoeba that undergoes a basic developmental program, and therefore analysis of RNA levels over a time course is a commonly used technique. This procedure is similar to other guanidine thiocyanate-based methods...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.191
更新日期：2007-01-01 00:00:00
abstract：:We describe the implementation and use of an adaptive imaging framework for optimizing spatial resolution and signal strength in a light-sheet microscope. The framework, termed AutoPilot, comprises hardware and software modules for automatically measuring and compensating for mismatches between light-sheet and detecti...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-018-0043-4
更新日期：2018-11-01 00:00:00
abstract：:Overexpression screens can be used to explore gene function in Drosophila melanogaster, but to demonstrate their full potential, comprehensive and systematic collections of fly strains are required. Here we provide a protocol for high-throughput cloning of Drosophila open-reading frames (ORFs) that are regulated by up...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2014.105
更新日期：2014-07-01 00:00:00
abstract：:Iterative saturation mutagenesis (ISM) is a new and efficient method for the directed evolution of functional enzymes. It reduces the necessary molecular biological work and the screening effort drastically. It is based on a Cartesian view of the protein structure, performing iterative cycles of saturation mutagenesis...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.72
更新日期：2007-01-01 00:00:00
