Abstract:
:This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the 2' position as a nitro-benzyl ether, can be incorporated into short RNAs by chemical synthesis or into long RNAs by a combination of chemical and enzymatic synthesis. The modified RNAs can be uncaged by photolysis under a variety of conditions including the use of a laser or xenon lamp, and the course of this uncaging reaction may be readily followed by HPLC or thin-layer chromatography.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Chaulk SG,MacMillan AMdoi
10.1038/nprot.2007.154subject
Has Abstractpub_date
2007-01-01 00:00:00pages
1052-8issue
5eissn
1754-2189issn
1750-2799pii
nprot.2007.154journal_volume
2pub_type
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