Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins.

Abstract:

:A procedure for the synthesis of N-succinimidyl 3-iodobenzoate labeled with any iodine isotope ([*I]SIB), which is an agent used in the radioiodination of proteins and peptides, from its tin precursor N-succinimidyl 3-(tri-n-butylstannyl)benzoate (STB) is described. Also included are protocols for the synthesis of an unlabeled standard of SIB and the tin precursor. Radioiododestannylation of STB using tert-butylhydroperoxide as the oxidant gives [*I]SIB in 80% radiochemical yields. The total time for the synthesis of [*I]SIB from STB is approximately 95 min. Use of [*I]SIB yields radioiodinated proteins that are considerably more stable in vivo than those radioiodinated by the direct electrophilic method.

journal_name

Nat Protoc

journal_title

Nature protocols

authors

Vaidyanathan G,Zalutsky MR

doi

10.1038/nprot.2006.99

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

707-13

issue

2

eissn

1754-2189

issn

1750-2799

pii

nprot.2006.99

journal_volume

1

pub_type

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