Abstract:
:A procedure for the synthesis of N-succinimidyl 3-iodobenzoate labeled with any iodine isotope ([*I]SIB), which is an agent used in the radioiodination of proteins and peptides, from its tin precursor N-succinimidyl 3-(tri-n-butylstannyl)benzoate (STB) is described. Also included are protocols for the synthesis of an unlabeled standard of SIB and the tin precursor. Radioiododestannylation of STB using tert-butylhydroperoxide as the oxidant gives [*I]SIB in 80% radiochemical yields. The total time for the synthesis of [*I]SIB from STB is approximately 95 min. Use of [*I]SIB yields radioiodinated proteins that are considerably more stable in vivo than those radioiodinated by the direct electrophilic method.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Vaidyanathan G,Zalutsky MRdoi
10.1038/nprot.2006.99subject
Has Abstractpub_date
2006-01-01 00:00:00pages
707-13issue
2eissn
1754-2189issn
1750-2799pii
nprot.2006.99journal_volume
1pub_type
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