Abstract：
:Desorption electrospray ionization (DESI) allows the direct analysis of ordinary objects or pre-processed samples under ambient conditions. Among other applications, DESI is used to identify and record spatial distributions of lipids and drug molecules in biological tissue sections. This technique does not require sample preparation other than production of microtome tissue slices and does not involve the use of ionization matrices. This greatly simplifies the procedure and prevents the redistribution of analytes during matrix deposition. Images are obtained by continuously moving the sample relative to the DESI sprayer and the inlet of the mass spectrometer. The timing of the protocol depends on the size of the surface to be analyzed and on the desired resolution. Analysis of organ tissue slices at 250 microm resolution typically takes between 30 min and 2 h.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Wiseman JM,Ifa DR,Venter A,Cooks RGdoi
10.1038/nprot.2008.11subject
Has Abstractpub_date
2008-01-01 00:00:00pages
517-24issue
3eissn
1754-2189issn
1750-2799pii
nprot.2008.11journal_volume
3pub_type
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