Abstract:
:This protocol describes the principles and methods used for the preparation of cryopreserved cell stocks. Following these procedures will ensure the availability of reproducible cultures for use within a single laboratory at different times and for different collaborating laboratories. Although the basic principle is simple, each cell line has characteristics that must to be borne in mind when establishing and testing such stocks. The key requirements are reliable methods for culture, cryopreservation, characterization and quality control.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Stacey GN,Masters JRdoi
10.1038/nprot.2008.190subject
Has Abstractpub_date
2008-01-01 00:00:00pages
1981-9issue
12eissn
1754-2189issn
1750-2799pii
nprot.2008.190journal_volume
3pub_type
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