Abstract:
:Here, we describe a simple micromolding method to construct three-dimensional arrays of organotypic epithelial tissue structures that approximate in vivo histology. An elastomeric stamp containing an array of posts of defined geometry and spacing is used to mold microscale cavities into the surface of type I collagen gels. Epithelial cells are seeded into the cavities and covered with a second layer of collagen. The cells reorganize into hollow tissues corresponding to the geometry of the cavities. Patterned tissue arrays can be produced in 3-4 h and will undergo morphogenesis over the following 1-3 d. The protocol can easily be adapted to study a variety of tissues and aspects of normal and neoplastic development.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Nelson CM,Inman JL,Bissell MJdoi
10.1038/nprot.2008.35subject
Has Abstractpub_date
2008-01-01 00:00:00pages
674-8issue
4eissn
1754-2189issn
1750-2799pii
nprot.2008.35journal_volume
3pub_type
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