Abstract:
:The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocomposite industries. We present analytical techniques not only to define the fine chemical structures of individual cell wall polysaccharides but also to estimate the overall polysaccharide composition of cell wall preparations. The procedure covers the preparation of cell walls, together with gas chromatography-mass spectrometry (GC-MS)-based methods, for both the analysis of monosaccharides as their volatile alditol acetate derivatives and for methylation analysis to determine linkage positions between monosaccharide residues as their volatile partially methylated alditol acetate derivatives. Analysis time will vary depending on both the method used and the tissue type, and ranges from 2 d for a simple neutral sugar composition to 2 weeks for a carboxyl reduction/methylation linkage analysis.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Pettolino FA,Walsh C,Fincher GB,Bacic Adoi
10.1038/nprot.2012.081subject
Has Abstractpub_date
2012-09-01 00:00:00pages
1590-607issue
9eissn
1754-2189issn
1750-2799pii
nprot.2012.081journal_volume
7pub_type
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