Abstract:
:In this protocol, we describe the preparation of nanoscale iron oxide-based materials and their use in the catalysis of different hydrogenation reactions. Pyrolysis of a Fe(OAc)2-phenanthroline complex on carbon at 800 °C under argon atmosphere results in the formation of nanoscale Fe2O3 particles surrounded by nitrogen-doped graphene layers. By applying these catalysts, the hydrogenation of structurally diverse and functionalized nitroarenes to anilines proceeds with excellent selectivity. Furthermore, we have shown that one-pot reductive amination of carbonyl compounds with nitroarenes is also possible in the presence of these iron oxide catalysts. We report herein the synthesis of more than 40 amines, which are important feedstocks and key intermediates for pharmaceuticals, agrochemicals and polymers. The detailed preparation of the catalysts and the procedures for the hydrogenation processes are presented. The overall time required for the catalyst preparation and for the hydrogenation reactions are 35 h and 20-35 h, respectively.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Jagadeesh RV,Stemmler T,Surkus AE,Junge H,Junge K,Beller Mdoi
10.1038/nprot.2015.025subject
Has Abstractpub_date
2015-04-01 00:00:00pages
548-57issue
4eissn
1754-2189issn
1750-2799pii
nprot.2015.025journal_volume
10pub_type
杂志文章相关文献
Nature Protocols文献大全abstract::Although differences in protein staining intensity can often be visualized by difference gel electrophoresis, abundant proteins can obscure less abundant proteins, and quantification of post-translational modifications is difficult. We present a protocol for quantifying changes in the abundance of a specific protein o...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2006.7
更新日期:2006-01-01 00:00:00
abstract::Here we present a protocol for labeling aliphatic carboxylic acids with the positron-emitting radionuclide 11C (t(1/2) = 20.4 min) at the carboxyl position using [11C]carbon monoxide via photoinitiated free radical-mediated carbonylation. A solution of an alkyl iodide in a homogenous binary organic solvent-water mixtu...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2006.112
更新日期:2006-01-01 00:00:00
abstract::Growing evidence indicates that RNA G-quadruplexes have important roles in various processes such as transcription, translation, regulation of telomere length, and formation of telomeric heterochromatin. Investigation of RNA G-quadruplex structures associated with biological events is therefore essential to understand...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2017.156
更新日期:2018-04-01 00:00:00
abstract::The scototaxis (dark/light preference) protocol is a behavioral model for fish that is being validated to assess the antianxiety effects of pharmacological agents and the behavioral effects of toxic substances, and to investigate the (epi)genetic bases of anxiety-related behavior. Briefly, a fish is placed in a centra...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2009.225
更新日期:2010-02-01 00:00:00
abstract::The tracking of cell fate, shape and migration is an essential component in the study of the development of multicellular organisms. Here we report a protocol that uses the protein Kaede, which is fluorescent green after synthesis but can be photoconverted red by violet or UV light. We have used Kaede along with confo...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2006.96
更新日期:2006-01-01 00:00:00
abstract::Post-translational protein nitration has attracted interest owing to its involvement in cellular signaling, effects on protein function and potential as biomarker of nitroxidative stress. We describe a procedure for enriching nitropeptides for mass spectrometry (MS)-based proteomics that is a simple and reliable alter...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2014.052
更新日期:2014-04-01 00:00:00
abstract::Human pluripotent stem cells (hPSCs) provide a valuable model for the study of human development and a means to generate a scalable source of cells for therapeutic applications. This protocol specifies cell fate efficiently into cardiac and endothelial lineages from hPSCs. The protocol takes 2 weeks to complete and re...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2016.153
更新日期:2017-01-01 00:00:00
abstract::A big challenge in proteomics is the identification of cell-type-specific proteomes in vivo. This protocol describes how to label, purify and identify cell-type-specific proteomes in living mice. To make this possible, we created a Cre-recombinase-inducible mouse line expressing a mutant methionyl-tRNA synthetase (L27...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/s41596-018-0106-6
更新日期:2019-02-01 00:00:00
abstract::Carbohydrate microarrays have received considerable attention as an advanced technology for the rapid analysis of carbohydrate-protein interactions. This protocol provides detailed procedures for the preparation of carbohydrate microarrays by immobilizing hydrazide-conjugated carbohydrates on epoxide-derivatized glass...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2007.373
更新日期:2007-01-01 00:00:00
abstract::The ability to rapidly generate large panels of antigen-specific human antibodies in a rodent would enable the efficient discovery of novel therapeutically useful antibodies. We have developed a system wherein human antigen-specific antibody-secreting plasmablasts can be enriched in vivo, in a severe combined immunode...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2014.104
更新日期:2014-07-01 00:00:00
abstract::Transitivity Clustering is a method for the partitioning of biological data into groups of similar objects, such as genes, for instance. It provides integrated access to various functions addressing each step of a typical cluster analysis. To facilitate this, Transitivity Clustering is accessible online and offers thr...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2010.197
更新日期:2011-03-01 00:00:00
abstract::Here we describe a protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient organ clearing, rapid imaging by light-sheet microscopy and quantitative imaging analysis of multiple samples. The organ or body is cleared by im...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2015.085
更新日期:2015-11-01 00:00:00
abstract::Protein digestion using a dedicated protease represents a key element in a typical mass spectrometry (MS)-based shotgun proteomics experiment. Up to now, digestion has been predominantly performed with trypsin, mainly because of its high specificity, widespread availability and ease of use. Lately, it has become appar...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2016.057
更新日期:2016-05-01 00:00:00
abstract::Aptamers are single-stranded DNA or RNA molecules that can bind target molecules with high affinity and specificity. The conformation of an aptamer usually changes upon binding to its target analyte, and this property has been used in a wide variety of sensing applications, including detection based on fluorescence in...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2006.38
更新日期:2006-01-01 00:00:00
abstract::Top-down proteomics (TDP) by mass spectrometry (MS) is a technique by which intact proteins are analyzed. It has become increasingly popDesalting and concentrating GELFrEEular in translational research because of the value of characterizing distinct proteoforms of intact proteins. Compared to bottom-up proteomics (BUP...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/s41596-018-0085-7
更新日期:2019-01-01 00:00:00
abstract::This protocol describes an efficient method to site-specifically label cell-surface or purified proteins with chemical probes in two steps: probe incorporation mediated by enzymes (PRIME) followed by chelation-assisted copper-catalyzed azide-alkyne cycloaddition (CuAAC). In the PRIME step, Escherichia coli lipoic acid...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2013.096
更新日期:2013-08-01 00:00:00
abstract::The partial reduction of electron-deficient pyrroles using either Birch (Li/NH(3)) or ammonia-free (Li/di-tert-butyl biphenyl) conditions allows formation of pyrroline compounds in good yield and, when combined with a reductive alkylation or similar approach, leads to highly functionalized, synthetically useful compou...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2007.245
更新日期:2007-01-01 00:00:00
abstract::RNA sequencing (RNA-seq) has been rapidly adopted for the profiling of transcriptomes in many areas of biology, including studies into gene regulation, development and disease. Of particular interest is the discovery of differentially expressed genes across different conditions (e.g., tissues, perturbations) while opt...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2013.099
更新日期:2013-09-01 00:00:00
abstract::An important goal of the analysis of sequenced genomes of microbial pathogens is to improve the therapy of infectious diseases. In this context, a major challenge is to detect genomic-level evolutionary changes that increase microbial virulence. TimeZone, a genome analysis software package, is designed to detect footp...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2013.031
更新日期:2013-04-01 00:00:00
abstract::A fluorescence-based microarray technique that does not require target DNA labeling is detailed. This 'label-free' approach utilizes a cationic, water-soluble conjugated polymer PFBT (poly[9,9'-bis(6''-(N,N,N-trimethylammonium)hexyl)fluorene-co-alt-4,7-(2,1,3-benzothiadiazole) dibromide]), and neutral PNA (peptide nuc...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2007.307
更新日期:2007-01-01 00:00:00
abstract::Differentiation of stem cells to hepatocytes has industrial applications, as well as the potential to develop new therapeutic strategies for liver disease. The protocol described here, sequentially using cytokines that are known to have a role in liver embryonic development, efficiently differentiates rat multipotent ...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2010.80
更新日期:2010-07-01 00:00:00
abstract::This protocol describes a cell-free system for studying vertebrate centromere and kinetochore formation. We reconstitute tandem arrays of centromere protein A (CENP-A) nucleosomes as a substrate for centromere and kinetochore assembly. These chromatin substrates are immobilized on magnetic beads and then incubated in ...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2012.112
更新日期:2012-10-01 00:00:00
abstract::Genome editing harnesses programmable nucleases to cut and paste genetic information in a targeted manner in living cells and organisms. Here, I review the development of programmable nucleases, including zinc finger nucleases (ZFNs), TAL (transcription-activator-like) effector nucleases (TALENs) and CRISPR (cluster o...
journal_title:Nature protocols
pub_type: 杂志文章,评审
doi:10.1038/nprot.2016.104
更新日期:2016-09-01 00:00:00
abstract::This protocol represents a novel enzyme-luminescence method to detect dopamine sensitively and rapidly with high temporal resolution. In principle, dopamine is first oxidized with tyramine oxidase to produce H(2)O(2), and then the produced H(2)O(2) reacts with luminol to generate chemiluminescence in the presence of h...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2008.158
更新日期:2008-01-01 00:00:00
abstract::This protocol describes the efficient, generally applicable Ullmann coupling reaction of bromaminic acid with alkyl- or aryl-amines in phosphate buffer under microwave irradiation using elemental copper as a catalyst. The reaction leads to a number of biologically active compounds. As a prototypical example, the synth...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2010.63
更新日期:2010-05-01 00:00:00
abstract::Live single-cell mass spectrometry (live MS) provides a mass spectrum that shows thousands of metabolite peaks from a single live plant cell within minutes. By using an optical microscope, a cell is chosen for analysis and a metal-coated nanospray microcapillary tip is used to remove the cell's contents. After adding ...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2015.084
更新日期:2015-09-01 00:00:00
abstract::Understanding chemical reactivity through the use of state-of-the-art computational techniques enables chemists to both predict reactivity and rationally design novel reactions. This protocol aims to provide chemists with the tools to implement a powerful and robust method for analyzing and understanding any chemical ...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/s41596-019-0265-0
更新日期:2020-02-01 00:00:00
abstract::Conventional gene expression studies analyze multiple cells simultaneously or single cells, for which the exact in vivo or in situ position is unknown. Although cellular heterogeneity can be discerned when analyzing single cells, any spatially defined attributes that underpin the heterogeneous nature of the cells cann...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2017.003
更新日期:2017-03-01 00:00:00
abstract::The forced swim test (FST) is one of the most commonly used animal models for assessing antidepressant-like behavior. This protocol details using the FST in rats, which takes place over 48 h and is followed by the video analysis of the behavior. The swim test involves the scoring of active (swimming and climbing) or p...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2012.044
更新日期:2012-05-03 00:00:00
abstract::Mass spectrometry (MS)-based proteomics has become the preferred tool for the analysis of protein phosphorylation. To be successful at such an endeavor, there is a requirement for an efficient enrichment of phosphopeptides. This is necessary because of the substoichiometric nature of phosphorylation at a given site an...
journal_title:Nature protocols
pub_type: 杂志文章
doi:10.1038/nprot.2013.010
更新日期:2013-03-01 00:00:00