Abstract:
:Over the past 20 years, the starlet sea anemone, Nematostella vectensis, a small estuarine animal, has emerged as a powerful model system for field and laboratory studies of development, evolution, genomics, molecular biology and toxicology. Here we describe how to collect Nematostella, culture it through its entire sexual life cycle and induce regeneration for the production of clonal stocks. In less than 1 h at a suitable field site, a researcher on foot can collect hundreds of individual anemones. In a few months, it is possible to establish a laboratory colony that will be reliable in generating hundreds or thousands of fertilized eggs on a roughly weekly schedule. By inducing regeneration roughly every 2 weeks, in less than 6 months, one can establish a clonal stock consisting of hundreds of genetically identical anemones. These results can be achieved very inexpensively and without specialized equipment.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Stefanik DJ,Friedman LE,Finnerty JRdoi
10.1038/nprot.2013.044subject
Has Abstractpub_date
2013-05-01 00:00:00pages
916-23issue
5eissn
1754-2189issn
1750-2799pii
nprot.2013.044journal_volume
8pub_type
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