Abstract:
:The goal of many current studies of neovascularization is to define the phenotype of vascular cell populations of different origins and to determine how such cells promote assembly of vascular channel. Here, we describe a protocol to immunophenotype vascular cells by high-resolution imaging and by fluorescence-activated flow cytometry in an in vivo rodent model of pulmonary microvascular remodeling. Analysis of cells by this combined approach will characterize their phenotype, quantify their number and identify their role in the assembly of vascular channels.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Jones RC,Capen DE,Cohen KS,Munn LL,Jain RK,Duda DGdoi
10.1038/nprot.2007.537subject
Has Abstractpub_date
2008-01-01 00:00:00pages
388-97issue
3eissn
1754-2189issn
1750-2799pii
nprot.2007.537journal_volume
3pub_type
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