Abstract：
:The effect of genetic mutation on phenotype is of significant interest in genetics. The type of genetic mutation that causes a single amino acid substitution (AAS) in a protein sequence is called a non-synonymous single nucleotide polymorphism (nsSNP). An nsSNP could potentially affect the function of the protein, subsequently altering the carrier's phenotype. This protocol describes the use of the 'Sorting Tolerant From Intolerant' (SIFT) algorithm in predicting whether an AAS affects protein function. To assess the effect of a substitution, SIFT assumes that important positions in a protein sequence have been conserved throughout evolution and therefore substitutions at these positions may affect protein function. Thus, by using sequence homology, SIFT predicts the effects of all possible substitutions at each position in the protein sequence. The protocol typically takes 5-20 min, depending on the input. SIFT is available as an online tool (http://sift.jcvi.org).
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Kumar P,Henikoff S,Ng PCdoi
10.1038/nprot.2009.86subject
Has Abstractpub_date
2009-01-01 00:00:00pages
1073-81issue
7eissn
1754-2189issn
1750-2799pii
nprot.2009.86journal_volume
4pub_type
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