Abstract:
:The increasing multi-resistance of pathogenic bacteria requires the development of novel classes of antibiotics. Antimicrobial host defense peptides represent one promising class. Here we describe a protocol for screening large numbers of peptides against any microbe of interest. Peptides synthesized on a cellulose support by peptide array technology can be added to a microbe that expresses the luxCDABE (luciferase) gene cassette. Any substance that decreases the energy level within the microbe will cause a quantifiable decrease in light production. The potency of the compound, at different concentrations, is reflected by the rate of decrease in luminescence. In conjunction with peptide array technology, the screening assay is rapid and high throughput and demonstrates good correlation with conventional killing or minimal inhibitory concentration assays performed with the same peptides synthesized by standard solid-phase peptide synthesis. The protocol can be completed in 3 d.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Hilpert K,Hancock REdoi
10.1038/nprot.2007.203subject
Has Abstractpub_date
2007-01-01 00:00:00pages
1652-60issue
7eissn
1754-2189issn
1750-2799pii
nprot.2007.203journal_volume
2pub_type
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