Abstract:
:We have compared the regulation of ornithine decarboxylase (ODC) gene expression in primary cultures of hamster embryo fibroblasts and in two independently transformed hamster embryo cell lines. Previous studies have demonstrated that 12-O-tetradecanoylphorbol-13-acetate (TPA) can greatly potentiate the serum growth factor induction of ODC enzyme activity in transformed cells, but not in normal hamster embryo fibroblasts. Treatment of either normal or transformed cells with both TPA and serum yielded greater accumulations of ODC mRNA than with either treatment alone, which is consistent with changes at the protein level. However, treatment of the transformed cells with TPA and serum resulted in a greater increase in steady state levels of ODC mRNA than that observed using normal fibroblasts. The time course for the induction of ODC mRNA was similar for both normal and transformed cells with maximal accumulations 4-8 h after treatment. Studies with actinomycin D further suggests that ODC mRNA is comparatively long-lived in both normal and transformed cells. The accumulation of ODC mRNA after stimulation with TPA and serum is blocked by cycloheximide in normal hamster fibroblasts suggesting that this induction is dependent upon protein synthesis. In contrast, cycloheximide did not affect the accumulation of ODC mRNA under similar treatment conditions in transformed cells. This altered regulation of ODC gene expression in transformed hamster embryo fibroblasts cannot be explained by either gene rearrangement or the amplification of an ODC gene. These data suggest that transformation of hamster embryo cells results in a loss of cellular control over ODC gene regulation which includes an alteration in the requirement for protein synthesis for ODC mRNA accumulation.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Gilmour SK,O'Brien TGdoi
10.1093/carcin/10.1.157subject
Has Abstractpub_date
1989-01-01 00:00:00pages
157-62issue
1eissn
0143-3334issn
1460-2180journal_volume
10pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Strains of laboratory mice vary markedly in their susceptibility to two-stage skin carcinogenesis using 7,12-dimethylbenz[a]anthracene (DMBA) as the initiating carcinogen and croton oil as promoter. This study has been undertaken in order to clarify the basis of the strain differences. LACA mice were used as the susce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.1.99
更新日期:1982-01-01 00:00:00
abstract::Studies with experimental animals indicate that acetaldehyde, the first metabolite of ethanol that is microbially formed in the colonic lumen, may play a role in ethanol-associated colorectal co-carcinogenesis. Although intracoIonic acetaldehyde concentrations are highest during the metabolism of exogenous ethanol, so...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.11.2031
更新日期:1998-11-01 00:00:00
abstract::Folate and vitamin D have been shown to be influenced by ultraviolet (UV) radiation. UVA radiation can break down plasma folate, whereas vitamin D can be synthesized in UVB-exposed skin. Folate metabolism is involved in DNA synthesis and repair, and vitamin D processes anti-proliferative effects. The functions of both...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl156
更新日期:2007-02-01 00:00:00
abstract::E mu-pim-1 transgenic mice, which overexpress the pim-1 oncogene in lymphoid tissues, have shown increased susceptibility to induction of T cell lymphomas by N-ethyl-N-nitrosourea, a direct-acting chemical carcinogen (Nature, 340, 61-63, 1989). We sought to further evaluate E mu-pim-1 transgenic mice as a potential te...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.2.285
更新日期:1995-02-01 00:00:00
abstract::Male and female strain A/J mice were exposed to a mixture of cigarette sidestream and mainstream smoke at a chamber concentration of total suspended particulates of 82.5 mg/m3. Exposure time was 6 h/day, 5 days/week for 5 months. The animals were allowed to recover for another 4 months in filtered air before sacrifice...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.10.1789
更新日期:1998-10-01 00:00:00
abstract::Transforming growth factor-beta 1 (TGF-beta1) plays a significant role in regulating the proliferation and apoptosis of prostate epithelial and stromal cells. We explored the association between the T (Leu) to C (Pro) polymorphism at codon10 of the TGF-beta1 gene (TGFB1) and the risk of prostate cancer (PCa) or benign...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg197
更新日期:2004-02-01 00:00:00
abstract::The HSP70 family of heat shock proteins consists of molecular chaperones of approximately 70kDa in size that serve critical roles in protein homeostasis. These adenosine triphosphatases unfold misfolded or denatured proteins and can keep these proteins in an unfolded, folding-competent state. They also protect nascent...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgt111
更新日期:2013-06-01 00:00:00
abstract::Base excision repair (BER) corrects DNA damage caused by oxidative stress and chronic inflammation, putative risk factors for cancer. To understand the relationship between genetic variation in BER genes and risk of biliary tract cancer and biliary stones, we examined non-synonymous polymorphisms in three key BER gene...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm247
更新日期:2008-01-01 00:00:00
abstract::Epidemiological studies have shown that a high incidence of oral cancers is associated with chewing betel quid. Since chromosomal aberrations are involved in many types of cancers, we investigated whether increased frequencies of chromosomal alterations could be detected in the oral mucosa cells of betel quid chewers ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.12.2347
更新日期:1997-12-01 00:00:00
abstract::Epidemiologic studies have suggested that aromatic amines (and nitroaromatic hydrocarbons) may be carcinogenic for human pancreas. Pancreatic tissues from 29 organ donors (13 smokers, 16 non-smokers) were examined for their ability to metabolize aromatic amines and other carcinogens. Microsomes showed no activity for ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.5.1085
更新日期:1997-05-01 00:00:00
abstract::A number of model systems have been developed to study the initiating and promoting phases of neoplastic development in rats liver. Four of these protocols use diethylnitrosamine (DEN) initiation, but employ different methods of promotion. The present studies were designed to evaluate these systems under standardized ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.8.851
更新日期:1982-01-01 00:00:00
abstract::Recent studies have revealed that 1,2,3,4,6-penta-O-galloyl-beta-d-glucose (PGG) has anti-tumorigenic activity in vitro. In the present work, we evaluated the in vitro and in vivo antiangiogenic and antitumor activities of PGG and examined its molecular mechanisms. PGG significantly inhibited the proliferation and tub...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi097
更新日期:2005-08-01 00:00:00
abstract::Insulin-like growth factor 1 receptor (IGF-1R) activation is required for prostate cell proliferation. Prostate cancer is one of the most commonly diagnosed malignant tumors in Western countries. Overexpression of IGF-1R in prostate cancer is associated with tumor growth. These suggest that IGF-1R inhibitory agents ma...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl189
更新日期:2007-03-01 00:00:00
abstract::Recent studies have shown opposing effects of n-6 and n-3 fatty acids on the development of cancer and suggest a role for the ratio of n-6 to n-3 fatty acids in the control of cancer. However, whether an alteration in the n-6/n-3 fatty acid ratio of cancer cells affects their invasive potential has not been well inves...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi019
更新日期:2005-04-01 00:00:00
abstract::Functional inactivation of BRCA1 is an important mechanism involved in breast cancer pathogenesis. Mutation is often responsible for BRCA1 inactivation in familial breast cancer, but is not responsible for the decreased levels of BRCA1 seen in a subset of sporadic breast cancer patients. To determine if aberrant cytos...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.9.1761
更新日期:2000-09-01 00:00:00
abstract::The nicotine-derived nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) induces lung adenocarcinoma through formation of DNA adducts. Our previous research on susceptibility to tobacco-induced carcinogenesis focused on benzo[a]pyrene diol epoxide (BPDE) as the in vitro mutagen for phenotype measurements ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm029
更新日期:2007-07-01 00:00:00
abstract::Wnt signaling plays a central role in mammary stem cell (MaSC) homeostasis and in breast cancer. In particular, epigenetic alterations at different members of the Wnt pathway have been identified among triple-negative, basal-like breast cancers. Previously, we developed a mouse model for metaplastic breast adenocarcin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt279
更新日期:2014-01-01 00:00:00
abstract::raf oncogenes have been implicated in hepatic carcinogenesis. We studied the effects of the v-raf of murine retrovirus 3611-MSV on the growth and differentiation of a simian virus 40 (SV40)-immortalized rat liver cell line (ALB-8) which maintained many of characteristics of differentiated hepatocytes. Cells were co-tr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.4.669
更新日期:1993-04-01 00:00:00
abstract::Celastrol is an anti-inflammatory natural triterpenoid, isolated from the herb Tripterygium wilfordii or thunder god vine. Here, we define mechanisms mediating anti-inflammatory activity of celastrol and demonstrate efficacy of a dietary celastrol supplement for chemoprevention of inflammation-driven carcinogenesis in...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx115
更新日期:2018-01-12 00:00:00
abstract::Precursor T-cell lymphoblastic neoplasms are aggressive malignancies in need for more effective and specific therapeutic treatments. A significant fraction of these neoplasms harbor deletions on the locus 9p21, targeting the tumor suppressor CDKN2A but also deleting the aconitase 1 (ACO1) gene, a neighboring housekeep...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgz185
更新日期:2020-08-12 00:00:00
abstract::Cytochrome P4501A1 (CYP1A1) has been implicated in the conversion of numerous polycyclic aromatic hydrocarbons into electrophilic species capable of binding covalently to DNA and has therefore been postulated to be involved in the initiation of carcinogenesis. The expression of CYP1A1 protein appears not to be constit...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.8.1361
更新日期:1998-08-01 00:00:00
abstract::The accumulation of O6-methylguanine (O6-meG) in the DNA of blood leukocytes of 21 Hodgkin's lymphoma patients (followed for up to 12 cycles of treatment) treated in the context of MOPP combination chemotherapy with 150 mg procarbazine daily for 10 days was examined and compared to that observed in rats treated with d...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.8.1675
更新日期:1994-08-01 00:00:00
abstract::Prostate cancer (CaP) is the second leading cause of cancer death in American men. Chronic inflammation has been one of several factors associated with the development of CaP. Single-nucleotide polymorphisms (SNPs) in cytokine genes have been associated with increased inflammation, increased cytokine production and po...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm277
更新日期:2008-03-01 00:00:00
abstract::Following intraperitoneal administration of [3H] aflatoxin B1 (AFB1) to young adult male rats, there is rapid uptake of the carcinogen by the liver, the target organ for carcinogenesis, leading to DNA covalent binding. Acid hydrolysis of this DNA shows that after 2h, the major DNA adduct is trans 8,9-dihydro-8-(7-guan...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.9.787
更新日期:1980-09-01 00:00:00
abstract::Karyotype, mitochondrial ultrastructure and several enzymatic activities were studied in two clones, D22 and D27, from SV40-transformed rabbit chondrocytes. Similar chromosome alterations, with recurrent losses and gains were observed at the various passages. Mitochondria were rare, with increase in size and crest alt...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.5.767
更新日期:1992-05-01 00:00:00
abstract::On the basis of preliminary in vitro experience, we assessed whether an enriched nutritional formulation with estrogen receptor (ER)-beta agonist and anti-inflammatory properties may prevent inflammation-associated colorectal cancer (CRC) in an animal model. Study sample enclosed 110 C57BL/6J male mice. Forty underwen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy104
更新日期:2018-10-08 00:00:00
abstract::Telomere dynamics are a critical component of both aging and cancer. Telomeres progressively shorten in almost all dividing cells and most human cells do not express or maintain sufficient telomerase activity to fully maintain telomeres. There is accumulating evidence that when only a few telomeres are short, they for...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgh296
更新日期:2005-05-01 00:00:00
abstract::Checkpoint kinase (CHEK) 2, a tumor suppressor gene, plays an essential role in the DNA damage checkpoint response cascade. We first investigated two polymorphisms in the proximal promoter of the CHEK2 gene and evaluated their associations with the risk of lung cancer in a case-control study using 500 incident lung ca...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq089
更新日期:2010-07-01 00:00:00
abstract::Phosphoinositide-specific phospholipase Cgamma (PLCgamma) is a key regulatory enzyme that binds to the phosphoryl-tyrosine residues in the cytoplasmic domain of certain activated receptors and catalyses the hydrolysis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] forming IP3 and diacylglycerol (DAG) in resp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.1.177
更新日期:1998-01-01 00:00:00
abstract::Arsenite is well established as a human carcinogen, but the molecular mechanisms leading to arsenite-induced carcinogenesis are complex and elusive. Accelerated glycolysis, a common process in tumor cells called the Warburg effect, is associated with various biological phenomena. However, the role of glycolysis induce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx034
更新日期:2017-06-01 00:00:00