Abstract:
:raf oncogenes have been implicated in hepatic carcinogenesis. We studied the effects of the v-raf of murine retrovirus 3611-MSV on the growth and differentiation of a simian virus 40 (SV40)-immortalized rat liver cell line (ALB-8) which maintained many of characteristics of differentiated hepatocytes. Cells were co-transfected with v-raf and the neo gene followed by selection with G418 for transfectants. In culture, the expression of v-raf stimulated cell proliferation without altering cell morphology or expression of liver-specific genes: albumin, fibrinogen, alpha-1-antitrypsin and alpha-1-acid glycoprotein. The v-raf-transfected cells induced rapidly growing tumors in 100% of nude mice, while control DNA-transfected cells were only weakly tumorigenic, producing slowly growing tumors in 2/7 mice after a long latency. These slowly growing tumors were histologically moderately to well-differentiated hepatocellular carcinomas in which the liver-specific genes were highly expressed. In contrast, v-raf-induced tumors were histologically poorly differentiated and showed a dramatic decline in the expression of the liver-specific genes. In a tumor cell culture established from a v-raf-induced tumor, however, expression of the liver-specific genes was coordinately recovered. These observations indicate that v-raf is capable of inducing progression of SV40-immortalized hepatocytes into highly malignant cells and the progression is accompanied by loss, in vivo, of the hepatic differentiation.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Fang XJ,Keating A,Flowers M,Liew CC,Gupta H,Mills GB,Sherman Mdoi
10.1093/carcin/14.4.669subject
Has Abstractpub_date
1993-04-01 00:00:00pages
669-74issue
4eissn
0143-3334issn
1460-2180journal_volume
14pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Oxidized DNA base lesions, such as thymine glycol (Tg) and 8-hydroxyguanine, are often toxic and mutagenic and have been implicated in carcinogenesis. To clarify whether NEIL1 protein, which exhibits excision repair activity towards such base lesions, is involved in gastric carcinogenesis, we examined 71 primary gastr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh267
更新日期:2004-12-01 00:00:00
abstract::A culture method to grow two morphologically distinguishable normal human breast epithelial cell types derived from reduction mammoplasty has been developed. Type I cells were characterized by a more variable cell shape, smooth cell colony boundaries, the expression of epithelial membrane antigen (EMA) and keratin 18 ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.3.531
更新日期:1995-03-01 00:00:00
abstract::Glioblastoma (GBM) is amongst the most aggressive brain tumors with a dismal prognosis. Despite significant advances in the current multimodality therapy including surgery, postoperative radiotherapy (RT) and temozolomide (TMZ)-based concomitant and adjuvant chemotherapy (CT), tumor recurrence is nearly universal with...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgy171
更新日期:2019-03-12 00:00:00
abstract::The Notch pathway plays a key role in the development and is increasingly recognized for its importance in cancer. We demonstrated previously the overexpression of Notch-1 and its ligands in gliomas and showed that their knockdown inhibits glioma cell proliferation and survival. To elucidate the mechanisms downstream ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn079
更新日期:2008-05-01 00:00:00
abstract::Carcinogenesis develops in stages that have been operationally defined as initiation, promotion and progression. Although morphological end points have been described for detection and quantitation of these stages, to date initiation has been assessed only in the context of clonal growth in response to certain promoti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.11.2587
更新日期:1994-11-01 00:00:00
abstract::The genes that are involved in estrogen biosynthesis, cellular binding and metabolism may contribute to breast cancer susceptibility. We examined the effect of the CYP17 promoter T --> C polymorphism and its interactions with the reproductive history, exogenous hormone use and selected lifestyle risk factors on breast...
journal_title:Carcinogenesis
pub_type: 杂志文章,多中心研究
doi:10.1093/carcin/bgn042
更新日期:2008-04-01 00:00:00
abstract::Oesophageal squamous cell carcinoma (OSCC) has a high incidence in southern Africa and a poor prognosis. Limited information is available on the contribution of genetic variants in susceptibility to OSCC in this region. However, recent genome-wide association studies have identified multiple susceptibility loci in Asi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgz026
更新日期:2019-06-10 00:00:00
abstract::Cyclin D1 is believed to play an important role in the genesis and/or progression of transitional cell cancer (TCC) of the urinary bladder. Cyclin D1 gene (CCND1) mRNA is alternatively spliced to produce two transcripts, and the splicing pattern may be modulated by a G to A single nucleotide polymorphism within the sp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.2.257
更新日期:2002-02-01 00:00:00
abstract::Dietary-related indoles, isothiocyanates, and the allyl isothiocyanate glucosinolate, sinigrin, were administered to F344 rats in the diet for 2 weeks (chronic protocol) or by gavage 2 h before sacrifice (acute protocol) and the effects of these pretreatments on the alpha-hydroxylation of two carcinogenic nitrosamines...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.4.539
更新日期:1985-04-01 00:00:00
abstract::Weanling male F344 rats were fed either a semi-purified diet low in methionine and lacking in choline and folic acid (folate/methyl deficient) or a supplemented control diet for periods of 2, 5, 7 days, 3 weeks, and 9 weeks. Two days after initiating the folate/methyl deficient diet in weanling F344 rats, the incidenc...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.2.287
更新日期:1997-02-01 00:00:00
abstract::Oral squamous cell carcinoma (SCC) is among the most prevalent cancers in the world and is characterized by high morbidity and few therapeutic options. Like most cancers, oral SCC arises from a multistep process involving alterations of genes responsible for balancing proliferation and differentiation. Among these, Kr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt349
更新日期:2014-03-01 00:00:00
abstract::Epidemiological studies have shown that a high incidence of oral cancers is associated with chewing betel quid. Since chromosomal aberrations are involved in many types of cancers, we investigated whether increased frequencies of chromosomal alterations could be detected in the oral mucosa cells of betel quid chewers ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.12.2347
更新日期:1997-12-01 00:00:00
abstract::A reduction in the ratio of tetraploid to diploid liver nuclei has been investigated as an early indicator of hepatocarcinogenesis in the rat using the liver carcinogen 3'-methyl-4-dimethylaminoazobenzene (3'M). In a dose ranging study 3'M was administered by gavage to rats at 5, 12.5 and 25 mg/kg for up to 10 weeks a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.1.21
更新日期:1985-01-01 00:00:00
abstract::To elucidate the mechanism of metabolic activation of 3'-methyl-N,N-dimethyl-4-aminoazobenzene (3'-Me-DAB), the mutagenicity of its metabolites and some related azo compounds towards Salmonella typhimurium TA-98 and TA-100 was investigated. Mutagenicity of the metabolites, either N-demethylated or oxidized at the 3'-m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.2.121
更新日期:1980-02-01 00:00:00
abstract::The purpose of this study was to determine if decreasing dietary protein from 24% (high protein) to 5% casein (low protein), substituting sucrose and cornstarch isocalorifically for casein, would modify the activity of protein kinase C (PKC) alpha and beta isoenzymes, as well as the expression of PKC alpha, beta, delt...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.5.1233
更新日期:1995-05-01 00:00:00
abstract::Hairless mice were given 5 topical applications of 470 nmol 20-methylcholanthrene (MCA) at one week intervals. In one experimental group the MCA was dissolved in reagent grade acetone alone, in another group it was dissolved in a mixture consisting of 50% acetone and 50% dimethyl sulfoxide (DMSO). A control group rece...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.11.1129
更新日期:1981-01-01 00:00:00
abstract::Mutations in genes important for the preservation of genome stability can increase the frequency of gene amplification, a process relevant to tumor development. To investigate whether telomerase, the enzyme deputed to telomere maintenance, also plays a role in gene amplification, we studied the amplification of the ca...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl158
更新日期:2007-03-01 00:00:00
abstract::The O6-alkylguanine-DNA alkyltransferase (ATase) is known to overcome the effects of promutagenic, precarcinogenic O6-alkylguanine induced in DNA by exposure to environmental, chemotherapeutic and dietary alkylating agents. Within an organ, the cell type-specific responses to these agents may be attributed, in part, t...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.4.737
更新日期:1993-04-01 00:00:00
abstract::Liver metastases develop in more than half of the patients with colorectal cancer (CRC) and are associated with a poor prognosis. The factors influencing liver metastasis of CRC are poorly characterized, but this information is urgently needed. We have now discovered that small extracellular vesicles (sEVs; exosomes) ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy115
更新日期:2018-12-13 00:00:00
abstract::A number of distinct subtypes of neuroblastoma exist with different genetic abnormalities that are predicative of outcome. Whole chromosome gains are usually associated with low stage disease and favourable outcome, whereas loss of 1p, 3p and 11q, unbalanced gain of 17q and MYCN amplification (MNA) are indicative of h...
journal_title:Carcinogenesis
pub_type: 杂志文章,多中心研究,评审
doi:10.1093/carcin/bgh173
更新日期:2004-09-01 00:00:00
abstract::3-tert-Butyl-4-hydroxyanisole (3-BHA) and its metabolites, tert-butyl-1,4-benzoquinone (BQ) and 3-tert-butyl-5-methoxy-1,2-benzoquinone (BHAoQ), were tested for initiating activity in a two-stage transformation assay using BALB/3T3 cells. Cells were treated first with a test chemical and then with the tumor promoter 1...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.11.1985
更新日期:1990-11-01 00:00:00
abstract::Exogenous chemical carcinogenesis is an extremely complex multifactorial process during which gene-environment interactions involving chronic exposure to exogenous chemical carcinogens (ECCs) and polymorphisms of cancer susceptibility genes add further complexity. We describe the properties and molecular mechanisms of...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgp252
更新日期:2010-02-01 00:00:00
abstract::2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is formed in cooked meats and may be linked to dietary-associated colorectal, prostate and mammary cancers. Genotoxic N-oxidized metabolites of PhIP react with the Cys34 of albumin (Alb) to form a sulfinamide adduct, a biomarker of the biologically effective dose....
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy125
更新日期:2018-12-31 00:00:00
abstract::Manganese superoxide dismutase (Mn-SOD) is strongly expressed in human ovarian cancer, and the serum level of the enzyme is a useful marker for the diagnosis and monitoring of human ovarian cancer. In the present study we found that Mn-SOD was highly expressed in primary and transplanted ovarian cancers in rats induce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.10.1941
更新日期:1992-10-01 00:00:00
abstract::Naturally occurring allyl isothiocyanate (AITC) was recently shown to be selectively delivered to bladder cancer tissue via urinary excretion and to inhibit bladder cancer growth and muscle invasion in an animal model. AITC is excreted in urine mainly as N-acetyl-S-(N-allylthiocarbamoyl)cysteine, more commonly known a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr283
更新日期:2012-02-01 00:00:00
abstract::We investigated possible mechanisms associated with promotion of hepatocarcinogenesis by the synthetic estrogens mestranol (M) and ethinyl estradiol (EE). Our first objective was to determine whether chronic EE treatment was associated with hepatotoxicity accompanied by regenerative hyperplasia. Female Sprague-Dawley ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.12.2007
更新日期:1986-12-01 00:00:00
abstract::Autophagy is an evolutionarily conserved mechanism to protect the cells from unfavorable environmental conditions. Inhibition of autophagy has been contemplated as a novel strategy to enhance anticancer efficacy of existing chemotherapeutic agents. We previously reported that pulsatilla saponin D (PSD) was a potent au...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgz140
更新日期:2020-07-10 00:00:00
abstract::The extent to which N-acetylbenzidine and N,N'-diacetylbenzidine are formed may influence benzidine-induced carcinogenesis. This study compared the formation of these metabolites by rat and human liver slices. The relationship between the NAT2 genotype and the formation of these acetylated products was also evaluated ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.7.1565
更新日期:1995-07-01 00:00:00
abstract::The nucleotide excision repair (NER) pathway is central in response to damage induced by environmental carcinogens. Efficiency of this pathway, probably genetically determined, may modulate individual risk of developing squamous cell carcinoma of the head and neck (SCCHN) as well as second primary malignancy (SPM) aft...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp096
更新日期:2009-06-01 00:00:00
abstract::The repair kinetics and sensitivity to inhibitors of DNA strand breaks caused by superoxide anion, hydrogen peroxide, benzoyl peroxide and anthralin have been studied and compared with strand breaks caused by well-studied agents such as ionizing radiation and bleomycin. The latter two agents are generally believed to ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.9.1511
更新日期:1986-09-01 00:00:00