当前位置： SCI文献检索 > CARCINOGENESIS期刊下所有文献 > N-acetylbenzidine and N,N'-diacetylbenzidine formation by rat and human liver slices exposed to benzidine.

N-acetylbenzidine and N,N'-diacetylbenzidine formation by rat and human liver slices exposed to benzidine.

Abstract：

:The extent to which N-acetylbenzidine and N,N'-diacetylbenzidine are formed may influence benzidine-induced carcinogenesis. This study compared the formation of these metabolites by rat and human liver slices. The relationship between the NAT2 genotype and the formation of these acetylated products was also evaluated in humans. In rat liver slices incubated with 0.05 mM [3H]benzidine for 1 h (n = 3), N-acetylbenzidine and N,N'-diacetylbenzidine represented 8.8 +/- 3.6 and 73 +/- 2.5% respectively of the total radioactivity recovered by HPLC. No unmetabolized benzidine was observed. This suggests that an equilibrium exists between benzidine, N-acetylbenzidine and N,N'-diacetylbenzidine in rat liver slice incubations which favors N,N'-diacetylbenzidine formation. In the presence of 0.1 mM paraoxon, a deacetylase inhibitor, N-acetylbenzidine and N,N'-diacetylbenzidine increased to 13 +/- 0.6 and 79 +/- 0.3% respectively. Within 2 h after incubating human liver slices with 0.014 mM [3H]benzidine (n = 8), benzidine, N-acetylbenzidine and N,N'-diacetylbenzidine represented 19 +/- 5, 34 +/- 4 and 1.6 +/- 0.5%, respectively, of the total radioactivity recovered by HPLC. Thus in the human, conditions in liver slices favor N-acetylbenzidine rather than N,N'-diacetylbenzidine formation. With paraoxon, benzidine, N-acetylbenzidine and N,N'-diacetylbenzidine represented 2 +/- 0.4, 24 +/- 4 and 51 +/- 3%, respectively. This resulted in a 32-fold increase in N,N'-diacetylbenzidine formation. Individuals with rapid NAT2 genotypes formed 1.4-fold more N-acetylbenzidine than slow acetylators. However, this increase was not significant. There was no apparent correlation of N,N'-diacetylbenzidine formation with NAT2 genotype. Similar results were observed when human slices were incubated with 0.09 mM [3H]benzidine. Deacetylase, perhaps more than N-acetyltransferase, influences hepatic metabolism and subsequent carcinogenesis of benzidine in man. These results help explain the species and organ specificity of benzidine carcinogenesis.

journal_name

Carcinogenesis

journal_title

Carcinogenesis

authors

Lakshmi VM,Bell DA,Watson MA,Zenser TV,Davis BB

doi

10.1093/carcin/16.7.1565

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

1565-71

issue

7

eissn

0143-3334

issn

1460-2180

journal_volume

16

pub_type

杂志文章

相关文献

CARCINOGENESIS文献大全
  • Mouse cell clones for improved quantitation of carcinogen-induced altered differentiation.

    abstract：:Two new mouse epidermal cell lines have been isolated and characterized as target cells for three chemical carcinogens. The ability to grow these cells at low density (approximately 5 clonogenic cells/cm2) has permitted more precise quantitation of chemical carcinogen-induced changes in epidermal differentiation. The ...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/6.9.1245

    authors： Kulesz-Martin M,Yoshida MA,Prestine L,Yuspa SH,Bertram JS

    更新日期：1985-09-01 00:00:00

  • Diethylnitrosamine administration in vivo increases hepatic poly(ADP-ribose) levels in rats: results of a modified technique for poly(ADP-ribose) measurement.

    abstract：:Poly(ADP-ribose) (polymer) is enzymatically synthesized on nuclear proteins in response to DNA strand breaks. NAD+ is the substrate for this reaction, which is catalyzed by poly(ADP-ribose)polymerase. This post-translational modification occurs in response to DNA strand breaks and is thought to play an important role ...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/14.12.2513

    authors： Rawling JM,Driscoll ER,Poirier GG,Kirkland JB

    更新日期：1993-12-01 00:00:00

  • Effects of ethinyl estradiol and tamoxifen on liver DNA turnover and new synthesis and appearance of gamma glutamyl transpeptidase-positive foci in female rats.

    abstract：:We investigated possible mechanisms associated with promotion of hepatocarcinogenesis by the synthetic estrogens mestranol (M) and ethinyl estradiol (EE). Our first objective was to determine whether chronic EE treatment was associated with hepatotoxicity accompanied by regenerative hyperplasia. Female Sprague-Dawley ...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/7.12.2007

    authors： Yager JD,Roebuck BD,Paluszcyk TL,Memoli VA

    更新日期：1986-12-01 00:00:00

  • Violacein synergistically increases 5-fluorouracil cytotoxicity, induces apoptosis and inhibits Akt-mediated signal transduction in human colorectal cancer cells.

    abstract：:Despite recent additions to the armory of chemotherapeutic agents for colorectal cancer (CRC) treatment, the results of chemotherapy remain unsatisfactory. 5-Fluorouracil (5-FU) still represents the cornerstone of treatment and resistance to its actions is a major obstacle to successful chemotherapy. Therefore, new ac...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgi307

    authors： Kodach LL,Bos CL,Durán N,Peppelenbosch MP,Ferreira CV,Hardwick JC

    更新日期：2006-03-01 00:00:00

  • Elevated polyamines lead to selective induction of apoptosis and inhibition of tumorigenesis by (-)-epigallocatechin-3-gallate (EGCG) in ODC/Ras transgenic mice.

    abstract：:Tea polyphenolic constituents induce apoptosis in cancer cells but not in normal cells. To study the mechanism of this selective effect, we used the ornithine decarboxylase (ODC)/Ras double transgenic mouse model that develops spontaneous skin tumors due to over-expression of ODC and a v-Ha-ras transgene. Administrati...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgh281

    authors： Paul B,Hayes CS,Kim A,Athar M,Gilmour SK

    更新日期：2005-01-01 00:00:00

  • Detection of N2,3-ethanoguanine in DNA after treatment with chloroacetaldehyde in vitro.

    abstract：:The reaction of chloroacetaldehyde, a reactive metabolic of the carcinogen vinyl chloride, with DNA produces in addition to the hitherto known adducts, 1,N6-ethenoadenine and 3,N4-ethenocytosine, an ethenoguanine adduct, namely N2,3-ethenoguanine. This adduct is formed in the reaction of chloroacetaldehyde with the fr...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/3.6.663

    authors： Oesch F,Doerjer G

    更新日期：1982-01-01 00:00:00

  • Hepatic and extrahepatic bioactivation and GSH conjugation of aflatoxin B1 in sheep.

    abstract：:Whole-body autoradiography of [3H]aflatoxin B1 ([3H]AFB1) in lamb showed a localization of bound labelling, in addition to the liver, in the nasal olfactory and respiratory mucosa, in the mucosa of the nasopharynx, pharynx, oesophagus, larynx, trachea, bronchi and bronchioles and in the palpebral and bulbar conjunctiv...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/15.5.947

    authors： Larsson P,Busk L,Tjälve H

    更新日期：1994-05-01 00:00:00

  • The coordinate regulation of DNA synthesis and suppression of apoptosis is differentially regulated by the liver growth agents, phenobarbital and methylclofenapate.

    abstract：:The coordinate regulation of DNA synthesis and suppression of apoptosis was investigated in a rat hepatocyte cell culture system which supports high level induction of DNA synthesis by the peroxisome proliferator, methylclofenapate (MCP) (Plant, N.J. et al., 1998, Carcinogenesis, 19, 925-931). The peroxisome prolifera...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/19.9.1521

    authors： Plant NJ,Horley NJ,Dickins M,Hasmall S,Elcombe CR,Bell DR

    更新日期：1998-09-01 00:00:00

  • Identification of two keratinocyte stem cell regulatory loci implicated in skin carcinogenesis.

    abstract：:Several converging lines of evidence suggest that keratinocyte stem cells including those clonogenic in vitro, are the target cells in skin carcinogenesis. However, the mechanism of their involvement in carcinogenesis continues to be unknown. Recently, we have demonstrated that the number of keratinocyte clonogenic st...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/24.3.417

    authors： Popova NV,Teti KA,Wu KQ,Morris RJ

    更新日期：2003-03-01 00:00:00

  • Inhibition of ultraviolet B (UVB)-induced c-fos and c-jun expression in vivo by a tyrosine kinase inhibitor genistein.

    abstract：:We reported the inhibitory effects of genistein, an inhibitor of tyrosine protein kinase (TPK), on ultraviolet B (UVB)-induced expression of c-fos and c-jun in SENCAR mouse skin. UVB irradiation substantially increased transcript levels of c-fos and c-jun mRNA in mouse skin. Topical application of genistein 60 min bef...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/19.4.649

    authors： Wang Y,Zhang X,Lebwohl M,DeLeo V,Wei H

    更新日期：1998-04-01 00:00:00

  • The oncoprotein ErbB3 is endocytosed in the absence of added ligand in a clathrin-dependent manner.

    abstract：:The oncoprotein ErbB3 is overexpressed in several human cancers, for example in pancreatic adenocarcinoma and in ovarian cancers, and ErbB3-containing heterodimers have been demonstrated to be potent signaling units in carcinogenesis. This especially applies to ErbB2-ErbB3 and epidermal growth factor receptor (EGFR)-E...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgs128

    authors： Sak MM,Breen K,Rønning SB,Pedersen NM,Bertelsen V,Stang E,Madshus IH

    更新日期：2012-05-01 00:00:00

  • Sensitivity of the conformation of deoxyguanosine to binding at the C-8 position by N-acetylated and unacetylated 2-aminofluorene.

    abstract：:The conformation of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene and N-(deoxyguanosin-8-yl)-2-aminofluorene was investigated by 1H n.m.r. spectroscopy. There was rotation about the glycosyl bond and preference for either the anti or syn conformation depended on whether or not the 8-arylamino nitrogen was acetylated. T...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/1.11.955

    authors： Evans FE,Miller DW,Beland FA

    更新日期：1980-01-01 00:00:00

  • Inhibition of 7,12-dimethylbenz[a]anthracene-induced lung tumorigenesis in A/J mice by food restriction is reversed by adrenalectomy.

    abstract：:Prior work has demonstrated that food restriction of mice markedly suppresses 12-O-tetradecanoylphorbol-13-acetate (TPA) promotion of skin papillomas and adrenalectomy prior to initiating food restriction completely reverses the tumor inhibitory effect of underfeeding. In the present experiment the effect of food rest...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/17.2.209

    authors： Pashko LL,Schwartz AG

    更新日期：1996-02-01 00:00:00

  • Fractionation of grape seed extract and identification of gallic acid as one of the major active constituents causing growth inhibition and apoptotic death of DU145 human prostate carcinoma cells.

    abstract：:The anti-cancer efficacy of grape seed extract (GSE) against prostate cancer (PCA) via its anti-proliferative, pro-apoptotic and anti-angiogenic activities in both cell culture and animal models have recently been described by us. GSE is a complex mixture containing gallic acid (GA), catechin (C), epicatechin (EC) and...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgi347

    authors： Veluri R,Singh RP,Liu Z,Thompson JA,Agarwal R,Agarwal C

    更新日期：2006-07-01 00:00:00

  • 32P-postlabeling analysis of adducts formed from 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) with DNA and homopolydeoxyribonucleotides.

    abstract：:A 32P-postlabeling assay was employed for detection and quantitation of DNA adducts formed with carcinogenic 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) activated by a peroxidase system. Enrichment of adducts by digestion with nuclease P1 or by extraction into n-butanol prior to 32P-labeling was used...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/13.7.1221

    authors： Stiborová M,Frei E,Schmeiser HH,Wiessler M

    更新日期：1992-07-01 00:00:00

  • Long-term tracking of hepatitis B viral load and the relationship with risk for hepatocellular carcinoma in men.

    abstract：:Little is known about the longitudinal course of hepatitis B virus (HBV) load and its relationship with the development of hepatocellular carcinoma (HCC). We conducted a case-cohort study nested within a cohort of 2874 HBV surface antigen-positive male Taiwanese government employees aged 30 years or older. HBV genotyp...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgm252

    authors： Wu CF,Yu MW,Lin CL,Liu CJ,Shih WL,Tsai KS,Chen CJ

    更新日期：2008-01-01 00:00:00

  • Inhibition of UV light- and Fenton reaction-induced oxidative DNA damage by the soybean isoflavone genistein.

    abstract：:We have investigated the effect of the soybean isoflavone genistein on 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation in calf thymus DNA exposed to either UV irradiation or the Fenton reaction system. Under the conditions used we observed that UV light and the Fenton reaction significantly increase 8-OHdG formation in...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/17.1.73

    authors： Wei H,Cai Q,Rahn RO

    更新日期：1996-01-01 00:00:00

  • Induction of hepatocyte proliferation by retinoic acid.

    abstract：:Retinoids have been shown to exert an anticarcinogenic effect through suppression of the cell cycle, induction of apoptosis and/or differentiation. In rat liver, in particular, retinoic acid has been shown to inhibit regeneration after partial hepatectomy, most probably through repression of the expression of c-fos an...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgh221

    authors： Ledda-Columbano GM,Pibiri M,Molotzu F,Cossu C,Sanna L,Simbula G,Perra A,Columbano A

    更新日期：2004-11-01 00:00:00

  • Mammary carcinogenesis in transgenic mice expressing a dominant-negative mutant of DNA polymerase beta in their mammary glands.

    abstract：:DNA polymerase beta (polbeta) is a major contributor to mammalian DNA damage repair through its gap-filling DNA synthesis and 5'-deoxyribose phosphate lyase activities. In this way, polbeta plays pivotal roles in the repair of oxidative DNA damage, replication, embryonic survival, neuronal development, meiosis, apopto...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgl239

    authors： Wang L,Bhattacharyya N,Rabi T,Wang L,Banerjee S

    更新日期：2007-06-01 00:00:00

  • Single nucleotide polymorphisms, metabolic activation and environmental carcinogenesis: why molecular epidemiologists should think about enzyme expression.

    abstract：:This commentary was written to stimulate thoughts on, and consideration of, enzyme expression data in target organs when investigating possible associations between polymorphisms in carcinogen activation enzymes, lifestyle/dietary factors and cancer risk. The lung and breast are taken as examples. There is overwhelmin...

    journal_title：Carcinogenesis

    pub_type： 杂志文章,评审

    doi：10.1093/carcin/22.2.209

    authors： Williams JA

    更新日期：2001-02-01 00:00:00

  • Pre-diagnostic leukocyte mitochondrial DNA copy number and skin cancer risk.

    abstract：:No previous study has examined the association between mitochondrial DNA copy number (mtCN) and skin cancer risk prospectively. We examined the associations between peripheral blood leukocytes mtCN level and the risks of skin cancers in a case-control study nested within the Nurses' Health Study of non-Hispanic White ...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgw072

    authors： Meng S,De Vivo I,Liang L,Giovannucci E,Tang JY,Han J

    更新日期：2016-09-01 00:00:00

  • Detection of acrolein and crotonaldehyde DNA adducts in cultured human cells and canine peripheral blood lymphocytes by 32P-postlabeling and nucleotide chromatography.

    abstract：:People are constantly being exposed to toxic and carcinogenic aldehydes. However, little is actually known about the mechanisms underlying the toxic and carcinogenic effects of these aldehydes on human cells. The DNA alkylating activities of two of the more toxic and environmentally prominent alpha,beta-unsaturated al...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/12.8.1483

    authors： Wilson VL,Foiles PG,Chung FL,Povey AC,Frank AA,Harris CC

    更新日期：1991-08-01 00:00:00

  • Diallyl disulfide (DADS) enhances gap-junctional intercellular communication by both direct and indirect mechanisms in rat liver cells.

    abstract：:Diallyl disulfide (DADS), a sulfur compound from garlic has been shown to exert many biological effects: induction of carcinogen detoxication, inhibition of tumor cell proliferation, etc. These effects are consistent with its anticarcinogenic properties in animal models and could account for garlic protective effects ...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgg182

    authors： Huard C,Druesne N,Guyonnet D,Thomas M,Pagniez A,Le Bon AM,Martel P,Chaumontet C

    更新日期：2004-01-01 00:00:00

  • Cyclooxygenase-2, malondialdehyde and pyrimidopurinone adducts of deoxyguanosine in human colon cells.

    abstract：:Cyclooxygenases (COX) catalyse the oxygenation of arachidonic acid to prostaglandin (PG) endoperoxides. Activity of one of the COX isoforms, COX-2, results in production of prostaglandin E(2) (PGE(2)) via the endoperoxide PGH(2). COX-2 has been implicated in the pathogenesis of colorectal cancer. Malondialdehyde (MDA)...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/22.9.1557

    authors： Sharma RA,Gescher A,Plastaras JP,Leuratti C,Singh R,Gallacher-Horley B,Offord E,Marnett LJ,Steward WP,Plummer SM

    更新日期：2001-09-01 00:00:00

  • Endogenous N-nitrosation in man assessed by measurement of apparent total N-nitroso compounds in faeces.

    abstract：:The faecal concentration of substances responding to the chemical test for N-nitroso compounds (apparent total N-nitroso compounds, ATNC) was investigated in human subjects consuming their normal free-choice diet. Concentrations ranged from 40 to 590 micrograms (N-NO)/kg faeces. To ascertain the likely relative contri...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/12.8.1395

    authors： Rowland IR,Granli T,Bøckman OC,Key PE,Massey RC

    更新日期：1991-08-01 00:00:00

  • Effects of green tea catechins in a rat multi-organ carcinogenesis model.

    abstract：:The effects of dietary administration of green tea catechins (GTC) were examined using a multi-organ carcinogenesis model. Groups of 15 F344 male rats were initially treated with a single i.p. administration of 100 mg/kg body wt N-diethyl-nitrosamine, 4 i.p. administrations of 20 mg/kg body wt N-methylnitrosourea, 4 s...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/14.8.1549

    authors： Hirose M,Hoshiya T,Akagi K,Takahashi S,Hara Y,Ito N

    更新日期：1993-08-01 00:00:00

  • Induction of melanoma in TPras transgenic mice.

    abstract：:In order to study the oncogenesis of melanocytes, transgenic mouse lines were established that express a mutated human Ha-ras (TPras) gene in pigment producing cells. The ras transgenic mice exhibit an altered phenotype, including melanocytic hyperplasia and a muted agouti coat, indicative of hyperproliferative melano...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/20.9.1747

    authors： Broome Powell M,Gause PR,Hyman P,Gregus J,Lluria-Prevatt M,Nagle R,Bowden GT

    更新日期：1999-09-01 00:00:00

  • Interaction of P53 Arg72Pro and MDM2 T309G polymorphisms and their associations with risk of gastric cardia cancer.

    abstract：:The P53 tumor suppressor pathway plays an important role in cancer development. The auto-regulatory feedback mechanism of the P53 and MDM2 expression is critical in keeping proper tumor suppressor function of this pathway. This study examined the effect of P53 Arg72Pro variants on transactivation of polymorphic MDM2 p...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/bgm168

    authors： Yang M,Guo Y,Zhang X,Miao X,Tan W,Sun T,Zhao D,Yu D,Liu J,Lin D

    更新日期：2007-09-01 00:00:00

  • Partial persistency of 2-aminofluorene and N-acetyl-2-aminofluorene in rat liver DNA.

    abstract：:Male rats were treated with [ring-3H]N-acetyl-2-aminofluorene and sacrificed at different periods of time after a single i.p. dose. Chromatin was isolated from liver homogenates and treated with RNAse and proteinase K. The resulting crude DNA was purified as the N-hexadecyl-N-trimethylammonium salt. The amounts of 2-a...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/2.8.737

    authors： Visser A,Westra JG

    更新日期：1981-01-01 00:00:00

  • Isolation of mutants temperature-sensitive for expression of the transformed state from chemically transformed C3H/10T1/2 cells.

    abstract：:58 MCA Cl 16 is an oncogenic methylcholanthrene-transformed variant of the non-transformed mouse embryo fibroblast cell line, C3H/10T1/2 Cl 8. Using two different protocols, we have isolated six temperature-sensitive mutants from N-methyl-N'-nitro-N-nitrosoguanidine treated cultures of 58 MCA Cl 16. C3H/10T1/2 Cl 8, 5...

    journal_title：Carcinogenesis

    pub_type： 杂志文章

    doi：10.1093/carcin/1.12.1059

    authors： Boreiko C,Heidelberger C

    更新日期：1980-01-01 00:00:00