Abstract:
:A 32P-postlabeling assay was employed for detection and quantitation of DNA adducts formed with carcinogenic 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) activated by a peroxidase system. Enrichment of adducts by digestion with nuclease P1 or by extraction into n-butanol prior to 32P-labeling was used. Both enrichment procedures exhibited comparable results for recovery of individual DNA adduct spots. Co-chromatographic analyses of adduct spots obtained by reaction with DNA and homopolydeoxy-ribonucleotides showed that four out of the eight major Sudan I-DNA adducts were formed by reaction of activated Sudan I with deoxyadenosine or deoxyguanosine in DNA. The accuracy of quantitation of adducts by 32P-postlabeling procedure is discussed.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Stiborová M,Frei E,Schmeiser HH,Wiessler Mdoi
10.1093/carcin/13.7.1221keywords:
subject
Has Abstractpub_date
1992-07-01 00:00:00pages
1221-5issue
7eissn
0143-3334issn
1460-2180journal_volume
13pub_type
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