Abstract:
:Several converging lines of evidence suggest that keratinocyte stem cells including those clonogenic in vitro, are the target cells in skin carcinogenesis. However, the mechanism of their involvement in carcinogenesis continues to be unknown. Recently, we have demonstrated that the number of keratinocyte clonogenic stem cells from mice is a genetically defined and quantitative complex trait. In this study, we analyzed the size of the colonies in BALB/c, C57BL/6, and their (BALB/cxC57BL/6)F1 hybrid mice. We found that the size of the colonies is also genetically regulated and that there are at least two types of clonogenic keratinocytes that form either small or large colonies. Small and large colony numbers were significantly different in BALB/c and C57BL/6 mice. We performed a genome-wide scan of small and large colony number in the two backcrosses [BALB/cx (BALB/cxC57BL/6)F1]; [C57BL/6x (BALB/cxC57BL/6) F1]; and [(BALB/cxC57BL/6)F1x (BALB/cxC57BL/6)F1] intercross mice. We report here that the loci on chromosomes 4 and 9 play a role in the regulation of large and small colony number respectively. We conclude that mouse epidermis has two major types of stem cells generating small and large colonies, and regulated by different genes. Our results suggest that the loci, including one major and several minor loci linked to the subpopulation of keratinocytes forming small colonies were related, if not identical to, the loci linked to susceptibility or resistance to skin tumor promotion and skin tumor development.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Popova NV,Teti KA,Wu KQ,Morris RJdoi
10.1093/carcin/24.3.417keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
417-25issue
3eissn
0143-3334issn
1460-2180journal_volume
24pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Since its discovery, the E3 ubiquitin ligase E6-associated protein (E6AP) has been studied extensively in two pathological contexts: infection by the human papillomavirus (HPV), and the neurodevelopmental disorder, Angelman syndrome. Vital biological links between E6AP and other viruses, namely hepatitis C virus and e...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgz072
更新日期:2019-07-06 00:00:00
abstract::We have investigated the activity of insoluble and soluble lead compounds in inducing mutagenesis, cell transformation and sister chromatid exchange in mammalian cells. Insoluble lead sulfide, readily phagocytized, was more than four times as toxic to V79 cells on a microM basis, than two moderately soluble lead compo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.10.1727
更新日期:1988-10-01 00:00:00
abstract::Woodchuck hepatocytes were immortalized with the simian virus 40 T antigen (SV40 T-ag) oncogene and utilized in an oncogenic transformation assay. Transfection of these cell lines with an activated C-Ha-ras oncogene (EJ6.6) resulted in the transformation of cells to a phenotype characterized by anchorage-independent g...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.4.631
更新日期:1996-04-01 00:00:00
abstract::Experiments were conducted to determine whether the natural estrogen and an environmental compound with estrogenic action, 4-n-octylphenol (4nOP), could modify tumor development in human c-Ha-ras proto-oncogene transgenic (Tg) rats which are highly susceptible to mammary and skin carcinogens. Female and male Tg and no...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.7.1209
更新日期:2002-07-01 00:00:00
abstract::Non-small cell lung cancer (NSCLC) is the leading cause of cancer mortality worldwide. Despite progress in developing chemotherapeutics for the treatment of NSCLC, primary and secondary resistance limits therapeutic success. NSCLC cells exhibit multiple mutations in the epidermal growth factor receptor (EGFR), which c...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt365
更新日期:2014-03-01 00:00:00
abstract::The effects of hyperoxia on lung tumor development were examined in mice and rats. In mice, exposure to 70% O2 prevented the development of urethan- or 3-methylcholanthrene-induced lung tumors. Dietary antioxidants [butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA)] were unable to prevent the inhibitio...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.9.1581
更新日期:1986-09-01 00:00:00
abstract::Changes in lipid metabolism were monitored in rat hepatocyte nodules at certain time points over 9 months. Tissue obtained from partially hepatectomized rats, collected over a period of 7 days, were included as a control for normal hepatocyte cell proliferation. Two important features regarding the lipid profiles of h...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.5.795
更新日期:2001-05-01 00:00:00
abstract::For a group of nine hydrocarbon and halogenated hydrocarbon epoxides, there is shown to be a good correlation between their abilities to inhibit epoxide hydrase and the quantity Vmin /Es, where Vmin is the most negative value of the molecule's electrostatic potential in the neighborhood of the epoxide oxygen and Es is...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.6.845
更新日期:1984-06-01 00:00:00
abstract::Arsenic is a human carcinogen whose mechanism of action is unknown. Previously, this laboratory demonstrated that arsenite acts as a comutagen by interfering with DNA repair, although a specific DNA repair enzyme sensitive to arsenite has not been identified. A number of stable arsenite-sensitive and arsenite-resistan...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.2.311
更新日期:1999-02-01 00:00:00
abstract::The Bowman-Birk protease inhibitor (BBI) is a legume-derived inhibitor of chymotrypsin and trypsin that has been shown to suppress cellular transformation and tumorigenesis. In the present investigation the effects of various BBI administration schedules were evaluated for suppression of 3-methylcholanthrene (3-MCA)-i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.5.935
更新日期:1991-05-01 00:00:00
abstract::We previously demonstrated that a single dose of gamma-radiation (350 rads) was able to induce thymic lymphomas in C57BL mice when followed by promoting treatment with oral cyclosporine (CsA), a non-genotoxic immunosuppressant. We have now tested the efficacy of various doses of gamma-radiation as an initiator of CsA ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.1.43
更新日期:1991-01-01 00:00:00
abstract::We have examined both the initial nuclease sensitivity and subsequent nucleosome rearrangement of newly repaired regions of chromatin in human diploid fibroblasts treated with methyl methanesulfonate (MMS) and methylnitrosourea (MNU). We initially examined the effect of these two alkylating agents on DNA replicative s...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.2.245
更新日期:1984-02-01 00:00:00
abstract::The effect of the protease inhibitor antipain (1.25 micrograms/ml) on the radiation-induced expression of a tumor-associated antigen in human cell hybrids has been investigated. A variety of treatment protocols have been studied where antipain was present before, during and at various times post-irradiation. It was fo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.12.2333
更新日期:1988-12-01 00:00:00
abstract::Covalent binding of [7-3H]styrene (S) to DNA in vivo was measured and evaluated in a quantitative manner in order to investigate whether DNA adduct formation could form a mechanistic basis for tumor induction in a carcinogenicity bioassay. [7-3H]S was administered by inhalation in a closed chamber to male and female C...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.3.355
更新日期:1993-03-01 00:00:00
abstract::Examination of p53 mutation spectra may provide clues to molecular mechanisms involved in different histologic types of gastric cancer. A total of 105 gastric cancer cases classified according to the Laurén's system were selected from a high-risk area around Florence, Italy. Exons 5-8 of the p53 gene were examined for...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.12.2145
更新日期:1998-12-01 00:00:00
abstract::Pleomorphic adenoma gene like-2 (PLAGL2), a member of the PLAG gene family, is a C2H2 zinc finger transcriptional factor that is involved in cellular transformation and apoptosis. In this report, we show that PLAGL2 is associated with the organization of stress fibers and with small guanosine triphosphatase (GTPase) a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgu081
更新日期:2014-09-01 00:00:00
abstract::N-nitrosopiperidine (NPIP) is a potent esophageal carcinogen in rats whereas structurally similar N-nitrosopyrrolidine (NPYR) induces liver, but not esophageal tumors. NPIP is a possible causative agent for human esophageal cancer. Our goal is to explain mechanistically these differing carcinogenic activities in the e...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.2.291
更新日期:2003-02-01 00:00:00
abstract::Previously, we reported the identification of a novel immunoglobulin-like cell adhesion molecule hepaCAM that is frequently downregulated and inhibits cell growth in hepatocellular carcinoma. In this study, we show that the expression of hepaCAM is suppressed in diverse human cancers. Aiming to evaluate the biological...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn226
更新日期:2008-12-01 00:00:00
abstract::p53 is a tumor suppressor that responds to a variety of stresses such as oncogenes and DNA damage by activating its transcriptional targets to allow repair or elimination of damaged cells. In the absence of stress signals, p53 needs to be kept in check and this is achieved by the E3 ligase MDM2. For tumors that retain...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs092
更新日期:2012-04-01 00:00:00
abstract::DNA-demethylating agents activate tumor suppressor genes that are silenced by DNA methylation in cancer and are therefore emerging as a novel approach to cancer therapy. 5-azacytidine (VIDAZA), the first representative of this class of drugs was approved for treatment of myelodysplastic syndromes and is currently bein...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt284
更新日期:2014-01-01 00:00:00
abstract::Carcinogen treatment of cultured mammalian cells prior to infection with u.v.-irradiated virus results in enhanced virus survival and mutagenesis suggesting the induction of SOS-type processes. In this paper, we report the development of a primary rat hepatocyte culture system to investigate cellular responses to DNA ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.4.495
更新日期:1984-04-01 00:00:00
abstract::It has previously been proposed that redox cycling between catechol estrogens and their quinones, mediated by cytochrome P450, could lead to the generation of free radicals that would subsequently cause oxidative damage to DNA and proteins that might have a role in hormonal carcinogenesis. Alternative, non-enzymatic m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.7.1421
更新日期:1994-07-01 00:00:00
abstract::The aim of this study has been to define cytotoxic mechanisms that may cause clonal expansion in the liver of pre-carcinogenic cells. An in vitro model, which has been described previously, was used. Hepatocytes were isolated from carcinogen-treated rats and a high proportion of the cells were gamma-glutamyltranspepti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.1.69
更新日期:1990-01-01 00:00:00
abstract::Syrian hamster embryo cells were treated with crocidolite asbestos at a dose (1 microgram/cm2) which was shown in previous studies to induce cell transformation and aneuploidy in these cells. Treatment of cells with asbestos induced a greater than 20-fold increase in the incidence of cells in anaphase with abnormaliti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.3.473
更新日期:1985-03-01 00:00:00
abstract::The human DNA polymerase lambda (Polλ) is a DNA repair polymerase, which is believed not only to play a role in base excision repair but also to contribute to DNA double-strand break repair by non-homologous end joining. We described here that cellular expression of the recently described natural polymorphic variant o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq166
更新日期:2010-10-01 00:00:00
abstract::Practical methods for the routine solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine are described. It is shown that if the 2-amino group of the alkylated base is protected with a phenylacetyl group, and a mixture of nitrobenzaldoximate ions and ammonia used to remove the protecting groups from ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.5.811
更新日期:1990-05-01 00:00:00
abstract::Recent studies have shown opposing effects of n-6 and n-3 fatty acids on the development of cancer and suggest a role for the ratio of n-6 to n-3 fatty acids in the control of cancer. However, whether an alteration in the n-6/n-3 fatty acid ratio of cancer cells affects their invasive potential has not been well inves...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi019
更新日期:2005-04-01 00:00:00
abstract::In the present study the role of two families of cytochrome P-450 proteins and the contribution of the cytosolic fraction in the activation of N-nitrosopiperidine to mutagens in the Ames test were investigated. The bioactivation of this nitrosamine was preferentially catalysed by the phenobarbitone-induced cytochromes...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.11.1691
更新日期:1987-11-01 00:00:00
abstract::Testosterone, testosterone propionate, 17 beta-trenbolone and progesterone, which represent the main endogenous and synthetic androgens and a progestin, were evaluated for possible cell transformation and genetic effects in Syrian hamster embryo (SHE) cells. Cell growth was reduced by treatment with the steroids at 10...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.6.1329
更新日期:1995-06-01 00:00:00
abstract::A mouse monoclonal antibody (Mab) was prepared which showed high specificity for a potential marker of exposure to methylating carcinogens such as 3-methyladenine (3-MeAde). In a low-temperature (4 degrees C) ELISA a linear calibration curve was obtained between 3 and 50 fmol/well. In combination with an immunoaffinit...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.10.1747
更新日期:1990-10-01 00:00:00