Abstract:
:Recent studies have revealed that 1,2,3,4,6-penta-O-galloyl-beta-d-glucose (PGG) has anti-tumorigenic activity in vitro. In the present work, we evaluated the in vitro and in vivo antiangiogenic and antitumor activities of PGG and examined its molecular mechanisms. PGG significantly inhibited the proliferation and tube formation in basic fibroblast growth factor (bFGF)-treated human umbilical vein endothelial cells (HUVECs) at non-cytotoxic concentrations. PGG effectively disrupted the bFGF-induced neo-vascularization in chick chorioallantoic membrane (CAM) and in Matrigel plugs in the mice. When mice were intraperitoneally injected, PGG also significantly inhibited tumor angiogenesis induced by Lewis lung carcinoma (LLC) and the growth of LLC by 57 and 91% of control tumor weight at 4 and 20 mg/kg, respectively. Immunohistochemical analysis revealed decreased microvessel density, decreased expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF), reduced tumor cell proliferation and increased tumor cell apoptosis. Similarly, PGG significantly attenuated the expression of COX-2 and VEGF and reduced the secretion of VEGF and prostaglandin E2 in bFGF-treated HUVECs. Furthermore, the COX-2 inhibitor NS398 significantly inhibited tube formation and neo-vascularization in CAM, supporting the role of COX-2 in PGG inhibition of angiogenesis. PGG diminished the phosphorylation of extracellular signal regulated kinase 1/2, Jun NH2-terminal kinase and activated phospho-p38 mitogen-activated protein kinase (MAPK) in a dose-dependent manner in bFGF-treated HUVECs. In addition, p38 inhibitor SB203580 abolished the downregulation of COX-2, VEGF and the antiproliferative activity by PGG. Taken together, our data demonstrate that PGG exerts antitumor activity primarily via inhibition of angiogenesis through COX-2 and MAPK- dependent pathways.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Huh JE,Lee EO,Kim MS,Kang KS,Kim CH,Cha BC,Surh YJ,Kim SHdoi
10.1093/carcin/bgi097keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
1436-45issue
8eissn
0143-3334issn
1460-2180pii
bgi097journal_volume
26pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::The carcinogenicities of 1-nitropyrene (1-NP), 4-nitropyrene (4-NP), 1,3-dinitropyrene (1,3-DNP), 1,6-dinitropyrene (1,6-DNP), 1,8-dinitropyrene (1,8-DNP), 3-hydroxy-1-nitropyrene (3-OH-1-NP) and a mixture of 6- and 8-hydroxy-1-nitropyrene (6/8-OH-1-NP) were investigated in newborn female rats. Newborn female CD rats ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.12.3027
更新日期:1995-12-01 00:00:00
abstract::Expression of the human placental form of glutathione S-transferase (GST-pi), an enzyme proposed as a marker for human and experimental neoplasia, was immunohistochemically evaluated in 51 samples of 'normal' and diseased adult human uterine cervix. Five fetal uteri were also studied. GST-pi positivity was detected in...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.12.2317
更新日期:1989-12-01 00:00:00
abstract::Recent studies have implicated multipotential mesenchymal stem cells (MSCs) as an aid to breast cancer cell proliferation and metastasis, partly as a result of the MSCs secretome. As the tumor gets beyond 2 mm in diameter, the stromal cells could undergo starvation due to the lack of sufficient nutrients in solid tumo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr029
更新日期:2011-07-01 00:00:00
abstract::Overexpression of the helix-loop-helix protein Id-1 has been reported in over 20 types of cancer. While a number of factors have been demonstrated to regulate Id-1 gene transcription, little is known about the mechanisms responsible for its degradation. In this study, we have demonstrated that Id-1 protein stability w...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi217
更新日期:2006-02-01 00:00:00
abstract::Experiments were conducted to determine whether the natural estrogen and an environmental compound with estrogenic action, 4-n-octylphenol (4nOP), could modify tumor development in human c-Ha-ras proto-oncogene transgenic (Tg) rats which are highly susceptible to mammary and skin carcinogens. Female and male Tg and no...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.7.1209
更新日期:2002-07-01 00:00:00
abstract::The influence of the smoking parameters (puff profile, puff duration, puff volume, puff frequency) on the delivery of tobacco-specific nitrosamines (TSNAs) in mainstream smoke was investigated for six different cigarette brands, including filter cigarettes with very low to medium smoke yields and non-filter cigarettes...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.6.1059
更新日期:1989-06-01 00:00:00
abstract::The Manchester (England) Children's Tumour Registry is population-based with a high level of ascertainment. Pathologic review ensured diagnostic accuracy. Routine monitoring of annual incidence revealed a significant increase in germ cell tumours from approximately 1 per million person years to approximately 4. Analys...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.1.75
更新日期:1982-01-01 00:00:00
abstract::Cancer-associated fibroblasts (CAFs) have been described to play critical roles in initiation, progression and metastasis of various cancers. However, the involvement of CAFs in oral cancer (OC) has not been well addressed. In this study, we demonstrate that CAFs, when cocultured with OC cells (OCCs), produce high lev...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgu046
更新日期:2014-06-01 00:00:00
abstract::2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a mutagenic and carcinogenic heterocyclic amine formed during ordinary cooking. PhIP is metabolically activated to the ultimate mutagenic metabolite by CYP P450-mediated N-hydroxylation followed by phase II esterification. Incubation of N-hydroxy-PhIP (N-OH-PhI...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::Cancer testis antigens (CTAs) are promising targets for T cell-based immunotherapy and studies have shown that certain CT genes are epigenetically depressed in cancer cells through DNA demethylation. Melanoma-associated antigen A11 (MAGE-A11) is a CTA that is frequently expressed in esophageal cancer and is correlated...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa057
更新日期:2020-07-14 00:00:00
abstract::Bone metastasis in patient with advanced-stage prostate cancer, the most commonly diagnosed malignancy in Western countries, increases the risk of intractable bone pain. The nephroblastoma overexpressed (NOV/CCN3) gene, a member of the CCN gene family, is responsible for the secretion of CCN3, a matrix-associated prot...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt103
更新日期:2013-07-01 00:00:00
abstract::Sixty-three male cigarette smokers were entered into a cross-sectional study to determine whether inverse associations existed between polycyclic aromatic hydrocarbon (PAH)-DNA adduct levels and intake/serum levels of vitamin A, vitamin C and vitamin E. Associations between PAH-DNA adducts and intakes of carotene, as ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.11.2449
更新日期:1994-11-01 00:00:00
abstract::N-Hydroxy-2-acetylaminofluorene (N-OH-AAF), N-hydroxy-4'-fluoro-4-acetylaminobiphenyl (N-OH-FAABP) and N-hydroxy-4-acetylaminobiphenyl (N-OH-AABP) were compared for their initiation and promotion activity in the rat liver using a modified Solt-Farber system. N-OH-AAF, N-OH-FAABP and N-OH-AABP showed comparable initiat...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.2.333
更新日期:1990-02-01 00:00:00
abstract::The role of urinary pH and Na+ concentration on the bladder carcinogenesis of o-phenylphenol (OPP) was examined in male F344 rats. The rats were given powdered diet containing 2% sodium o-phenylphenate (OPP-Na, group 1), 1.25% OPP plus 0.64% NaHCO3 (group 2), 1.25% OPP plus 0.32% NaHCO3 (group 3), 1.25% OPP plus 0.16%...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.9.1635
更新日期:1989-09-01 00:00:00
abstract::Previous studies have shown that 1- and 3-nitrobenzo[a]pyrene (NBaP) were mutagenic in the Salmonella reversion assay without exogenous activation and that 1-, 3- and 6-NBaP were mutagenic in the presence of hepatocytes or liver homogenate (S9). In the present study, 1-, 3- and 6-NBaP were tested for mutagenicity in C...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.4.681
更新日期:1986-04-01 00:00:00
abstract::Possible relationships between structure and metabolism of nitrosamines have been investigated in the rat small intestine. Isolated segments of jejunum and ileum were perfused from the luminal side for 2 h with a Tyrode solution containing one of four symmetrical dialkylnitrosamines with 2-5 carbon atoms per side chai...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.7.1207
更新日期:1986-07-01 00:00:00
abstract::In vivo incorporation of [3H] thymidine into DNA was determined in rats at 28 h after partial hepatectomy. Administration of nickel carbonyl (Ni(CO)4) at 2 or 4 h before sacrifice inhibited [3H] thymidine uptake into liver and kidney DNA. For example, in rats killed 4 h after i.v. injection of Ni(CO)4 (2 mg Ni/100 g),...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.4.297
更新日期:1980-04-01 00:00:00
abstract::Certain hexavalent chromium [Cr(VI)] compounds are implicated as occupational respiratory carcinogens. Cr(VI) induces a broad spectrum of DNA damage, but Cr(VI)-induced DNA double-strand breaks (DSBs) have not been reported. Previously we found that Cr(VI) activates the ataxia telangiectasia mutated (ATM) kinase. ATM ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh242
更新日期:2004-11-01 00:00:00
abstract::Occupational nitrosamine exposures from a rubber vehicle seal (VS) curing operation were compared with the peripheral blood lymphocyte concentrations of two nitrosamine-related DNA adducts, N(7)-methylguanine (N(7)mdG) and O(6)-methylguanine (O(6)mdG), and with the activity of the enzyme that repairs O(6)mdG adducts, ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.1.29
更新日期:2000-01-01 00:00:00
abstract::Staurosporine (stsp) is a kinase inhibitor which induces cornified envelope assembly and terminal differentiation in normal and neoplastic mouse keratinocytes. In the tumorigenic cell lines 308 and SP-1 experiments were performed to determine if this effect was due only to activation of transglutaminases (TGases) alre...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.6.1259
更新日期:1996-06-01 00:00:00
abstract::Recent studies have demonstrated that K-ras mutations in lung epithelial cells elicit inflammation that promotes carcinogenesis in mice (intrinsic inflammation). The finding that patients with chronic obstructive pulmonary disease (COPD), an inflammatory disease of the lung, have an increased risk of lung cancer after...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp229
更新日期:2009-11-01 00:00:00
abstract::Dog urinary bladder is a target organ of carcinogenic arylamines. However, dog hepatic and urothelial cytosols lack acetylation enzymes that are capable of activating N-hydroxy metabolites of arylamines, suggesting that other enzymes may be involved. In the present study, we found that dog liver microsomes were capabl...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.10.1887
更新日期:1991-10-01 00:00:00
abstract::The mechanism(s) of tumour promotion in liver by estrogens is not well understood although growth stimulation is known to be one important element of their action. As a basis for studying mechanisms of growth control by estrogens, effects of both natural and synthetic estrogens on DNA synthesis and protooncogene c-fos...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.9.1473
更新日期:2001-09-01 00:00:00
abstract::Neuroblastic tumors (NTs) include the neuroblastomas, ganglioneuroblastomas and ganglioneuromas. We have reported previously that the calcium-sensing receptor is expressed in differentiated, favorable NTs but almost undetectable in unfavorable neuroblastomas. We have now detected hypermethylation of a particular regio...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs338
更新日期:2013-02-01 00:00:00
abstract::The possible roles of cytochrome P450 (P450) enzymes in the metabolic activation of N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) by rat liver microsomes have been examined in a system containing the bacterial tester strain Salmonella typhimurium NM2009, a newly developed strain showing high O-acetylt...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.6.979
更新日期:1992-06-01 00:00:00
abstract::We measured the mutation frequency and spectrum induced by exposure of the mutation reporter plasmid pSP189 in vivo to phorbol myristate acetate (PMA)-activated human polymorphonuclear leukocytes (PMNs). The mutation frequency induced in the supF tRNA gene of pSP189 transfected into human Ad293 cells by a 30 min expos...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.10.2137
更新日期:1996-10-01 00:00:00
abstract::Phenobarbital (PB) is a potent tumor promoter in rodent liver. In this study we investigated whether PB selectively promotes a population of initiated cells with reduced levels of transforming growth factor-beta (TGF beta) receptors types I, II and III. Liver tumors were induced in male Fischer F344 rats by diethylnit...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.1.171
更新日期:1996-01-01 00:00:00
abstract::Intake of dietary factors that serve as methyl group donors may influence promoter hypermethylation in colorectal carcinogenesis. We investigated whether dietary folate, vitamin B2 and vitamin B6, methionine and alcohol were associated with mutL homologue 1 (MLH1) hypermethylation and the related molecular phenotypes ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn074
更新日期:2008-09-01 00:00:00
abstract::Previous reports showed that skin fibroblasts or peripheral blood lymphocytes from individuals with hereditary cancer or with a genetic disorder predisposing to cancer show an abnormally high frequency of chromatid damage after X-irradiation in G2 phase. The reproducibility of this response suggested that it could pro...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.8.1425
更新日期:1990-08-01 00:00:00
abstract::Mutations or transcriptional silencing of mismatch repair genes have been linked with tumour cell resistance to O(6)-guanine methylating agents, 6-thioguanine, cisplatin, doxorubicin and etoposide. Recently, it has been demonstrated that overexpression of the MSH3 protein is associated with depletion of the mismatch b...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.8.1131
更新日期:2001-08-01 00:00:00