The Xist RNA-PRC2 complex at 20-nm resolution reveals a low Xist stoichiometry and suggests a hit-and-run mechanism in mouse cells.

abstract：:Separation of cells is a critical process for studying cell properties, disease diagnostics, and therapeutics. Cell sorting by acoustic waves offers a means to separate cells on the basis of their size and physical properties in a label-free, contactless, and biocompatible manner. The separation sensitivity and effici...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ding X,Peng Z,Lin SC,Geri M,Li S,Li P,Chen Y,Dao M,Suresh S,Huang TJ

更新日期：2014-09-09 00:00:00

abstract：:Nicotine exposure diminishes the protective breathing and arousal responses to stress (hypoxia). By exacerbating sleep-disordered breathing, this disturbance could underpin the well established association between smoking and the increased risk of sudden infant death syndrome. We show here that the protective response...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen G,Han ZY,Grailhe R,Gallego J,Gaultier C,Changeux JP,Lagercrantz H

更新日期：2002-10-01 00:00:00

abstract：:Non-protein-coding RNA genes play an important role in various biological processes. How new RNA genes originated and whether this process is controlled by similar evolutionary mechanisms for the origin of protein-coding genes remains unclear. A young chimeric RNA gene that we term sphinx (spx) provides the first insi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang W,Brunet FG,Nevo E,Long M

更新日期：2002-04-02 00:00:00

abstract：:The tremendous pandemic potential of coronaviruses was demonstrated twice in the past few decades by two global outbreaks of deadly pneumonia. The coronavirus spike (S) glycoprotein initiates infection by promoting fusion of the viral and cellular membranes through conformational changes that remain largely uncharacte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walls AC,Tortorici MA,Snijder J,Xiong X,Bosch BJ,Rey FA,Veesler D

更新日期：2017-10-17 00:00:00

abstract：:Overaccumulation of abnormally organized mitochondria in so-called "ragged-red" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial encephalomyopathies. Characteristic for the abnormal mitochondria is the occurrence of highly ordered crystalline inclusions. Im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stadhouders AM,Jap PH,Winkler HP,Eppenberger HM,Wallimann T

更新日期：1994-05-24 00:00:00

abstract：:A selenium-containing protein, selenoprotein A, is an essential component of the clostridial glycine reductase complex. This enzyme complex catalyzes the reductive deamination of glycine, which is coupled to the esterification of orthophosphate resulting in the formation of ATP. Sequence information was obtained by au...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sliwkowski MX,Stadtman TC

更新日期：1988-01-01 00:00:00

abstract：:Biodiversity responses to ongoing climate and atmospheric changes will affect both ecosystem processes and the delivery of ecosystem goods and services. Combined effects of co-occurring global changes on diversity, however, are poorly understood. We examined plant diversity responses in a California annual grassland t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zavaleta ES,Shaw MR,Chiariello NR,Mooney HA,Field CB

更新日期：2003-06-24 00:00:00

abstract：:Chronic fatigue immune dysfunction syndrome (CFIDS) is a recently recognized illness characterized by debilitating fatigue as well as immunological and neurological abnormalities [Straus, S.E. (1988) J. Inf. Dis. 157, 405-412]. Once thought to be caused by Epstein-Barr virus, it is now thought to have a different but ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeFreitas E,Hilliard B,Cheney PR,Bell DS,Kiggundu E,Sankey D,Wroblewska Z,Palladino M,Woodward JP,Koprowski H

更新日期：1991-04-01 00:00:00

abstract：:With efforts underway to develop a preventive human immunodeficiency virus type 1 (HIV-1) vaccine, it remains unclear which immune responses are sufficient to protect against infection and whether prior HIV-1 immunity can alter the subsequent course of HIV-1 infection. We investigated these issues in the context of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McElrath MJ,Corey L,Greenberg PD,Matthews TJ,Montefiori DC,Rowen L,Hood L,Mullins JI

更新日期：1996-04-30 00:00:00

abstract：:Chronic wounds represent a substantial public health problem. The development of tools that would enable sophisticated scrutiny of clinical wound tissue material is highly desirable. This work presents evidence enabling rapid specific identification and laser capture of blood vessels from human tissue in a manner whic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy S,Patel D,Khanna S,Gordillo GM,Biswas S,Friedman A,Sen CK

更新日期：2007-09-04 00:00:00

abstract：:Crosses between closely related animal species often result in male hybrids that are sterile, and the molecular and functional basis of genetic factors for hybrid male sterility is of great interest. Here, we report a molecular and functional analysis of HMS1, a region of 9.2 kb in chromosome 3 of Drosophila mauritian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liénard MA,Araripe LO,Hartl DL

更新日期：2016-07-19 00:00:00

abstract：:A simple protocol for the rescue of a synthetic genome into a paramyxovirus has been developed. First, a synthetic Sendai virus-like RNA, containing the antisense coding region of the chloramphenicol acetyltransferase gene replacing the coding region of the Sendai virus genome, was transcribed from a cDNA. When introd...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park KH,Huang T,Correia FF,Krystal M

更新日期：1991-07-01 00:00:00

abstract：:Studies of perception usually emphasize processes that are largely universal across observers and--except for short-term fluctuations--stationary over time. Here we test the universality and stationarity assumptions with two families of ambiguous visual stimuli. Each stimulus can be perceived in two different ways, pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wexler M,Duyck M,Mamassian P

更新日期：2015-12-01 00:00:00

abstract：:Previously we reported that pretreatment of rats with the substance P (SP) antagonist CP-96,345 inhibits the enterotoxic responses following administration of toxin A from Clostridium difficile into ileal loops, indicating that SP participates in the intestinal responses to this toxin. We now report that injection of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castagliuolo I,Keates AC,Qiu B,Kelly CP,Nikulasson S,Leeman SE,Pothoulakis C

更新日期：1997-04-29 00:00:00

abstract：:Although much is known about the underlying mechanisms of p53 activity and regulation, the factors that influence the diversity and duration of p53 responses are not well understood. Here we describe a unique mode of p53 regulation involving alternative splicing of the TP53 gene. We found that the use of an alternativ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Senturk S,Yao Z,Camiolo M,Stiles B,Rathod T,Walsh AM,Nemajerova A,Lazzara MJ,Altorki NK,Krainer A,Moll UM,Lowe SW,Cartegni L,Sordella R

更新日期：2014-08-12 00:00:00

abstract：:Glycogen synthase kinase 3 (GSK-3) is implicated in multiple biological processes including metabolism, gene expression, cell fate determination, proliferation, and survival. GSK-3 activity is inhibited through phosphorylation of serine 21 in GSK-3 alpha and serine 9 in GSK-3 beta. These serine residues of GSK-3 have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fang X,Yu SX,Lu Y,Bast RC Jr,Woodgett JR,Mills GB

更新日期：2000-10-24 00:00:00

abstract：:Macrophage responses to Francisella infection have been characterized previously by subdued proinflammatory responses; however, these studies have generally focused on macrophage cell lines or monocyte-derived macrophages. Therefore, we studied the ability of fresh human blood monocytes to engulf and respond to Franci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gavrilin MA,Bouakl IJ,Knatz NL,Duncan MD,Hall MW,Gunn JS,Wewers MD

更新日期：2006-01-03 00:00:00

abstract：:We have examined components of the preintegration complex of human immunodeficiency virus type 1 (HIV-1) and have analyzed features which govern the association of these components. HIV-1 nucleoprotein complexes, isolated from nuclear and cytoplasmic extracts of CD4+ cells after acute virus infection, contained viral ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bukrinsky MI,Sharova N,McDonald TL,Pushkarskaya T,Tarpley WG,Stevenson M

更新日期：1993-07-01 00:00:00

abstract：:The density of benzodiazepine/gamma-aminobutyric acid receptor binding sites was lower in the midbrain of seizure-susceptible gerbils compared to control seizure-resistant gerbils. Binding of [3H]diazepam to high-affinity brain-specific sites in membrane homogenates of gerbil brain showed a 20-30% lower binding in mid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olsen RW,Wamsley JK,McCabe RT,Lee RJ,Lomax P

更新日期：1985-10-01 00:00:00

abstract：:Horizontal drilling and hydraulic fracturing have enhanced energy production but raised concerns about drinking-water contamination and other environmental impacts. Identifying the sources and mechanisms of contamination can help improve the environmental and economic sustainability of shale-gas extraction. We analyze...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Darrah TH,Vengosh A,Jackson RB,Warner NR,Poreda RJ

更新日期：2014-09-30 00:00:00

abstract：:Modified bovine leukemia virus (BLV) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. This analysis indicates that (i) both BLV envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the N-termi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vonèche V,Portetelle D,Kettmann R,Willems L,Limbach K,Paoletti E,Ruysschaert JM,Burny A,Brasseur R

更新日期：1992-05-01 00:00:00

abstract：:Within the Ig superfamily (IgSF), intercellular adhesion molecules (ICAMs) form a subfamily that binds the leukocyte integrin alphaLbeta2. We report a 1.65-A-resolution crystal structure of the ICAM-3 N-terminal domain (D1) in complex with the inserted domain, the ligand-binding domain of alphaLbeta2. This high-resolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song G,Yang Y,Liu JH,Casasnovas JM,Shimaoka M,Springer TA,Wang JH

更新日期：2005-03-01 00:00:00

abstract：:New advances in polarized light microscopy were used to image Congo red-stained cerebral amyloidosis in sharp relief. The rotating-polarizer method was used to separate the optical effects of transmission, linear birefringence, extinction, linear dichroism, and orientation of the electric dipole transition moments and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin LW,Claborn KA,Kurimoto M,Geday MA,Maezawa I,Sohraby F,Estrada M,Kaminksy W,Kahr B

更新日期：2003-12-23 00:00:00

abstract：:Huntington disease (HD) is caused by an expansion mutation of the N-terminal polyglutamine of huntingtin (mHTT). mHTT is ubiquitously present, but it induces noticeable damage to the brain's striatum, thereby affecting motor, psychiatric, and cognitive functions. The striatal damage and progression of HD are associate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma M,Rajendrarao S,Shahani N,Ramírez-Jarquín UN,Subramaniam S

更新日期：2020-07-07 00:00:00

abstract：:Once-abundant aerial insectivores, such as the Tree Swallow (Tachycineta bicolor), have declined steadily in the past several decades, making it imperative to understand all aspects of their ecology. Aerial insectivores forage on a mixture of aquatic and terrestrial insects that differ in fatty acid composition, speci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Twining CW,Brenna JT,Lawrence P,Shipley JR,Tollefson TN,Winkler DW

更新日期：2016-09-27 00:00:00

abstract：:Mutations of von Hippel-Lindau disease (VHL) tumor-suppressor gene product (pVHL) are found in patients with dominant inherited VHL syndrome and in the vast majority of sporadic clear cell renal carcinomas. The function of the pVHL protein has not been clarified. pVHL has been shown to form a complex with elongin B an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iwai K,Yamanaka K,Kamura T,Minato N,Conaway RC,Conaway JW,Klausner RD,Pause A

更新日期：1999-10-26 00:00:00

abstract：:The objective of this study was to determine whether the vascular smooth muscle contractile effect of NG-methyl-L-arginine (NMA) is endothelium dependent and attributed to a decline in smooth muscle levels of cyclic GMP. Vascular smooth muscle levels of cyclic GMP are severalfold greater in endothelium-intact than in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gold ME,Wood KS,Byrns RE,Fukuto J,Ignarro LJ

更新日期：1990-06-01 00:00:00

abstract：:Egg activation is the process in which mature oocytes are released from developmental arrest and gain competency for embryonic development. In Drosophila and other arthropods, eggs are activated by mechanical pressure in the female reproductive tract, whereas in most other species, eggs are activated by fertilization....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Q,Wolfner MF

更新日期：2019-09-17 00:00:00

abstract：:We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of EcoRI digests and were arranged in a series of overlapping fragments using the EcoRI fragment map of F established by H. O...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skurray RA,Nagaishi H,Clark AJ

更新日期：1976-01-01 00:00:00

abstract：:A method is proposed for rapidly, quantitatively comparing protein structures of arbitrary sizes, based on the differential geometric representation. The method is applied to a group of 22 protein x-ray structures, and the resulting network of closest relationships is delineated. Several well-known fold types are auto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S,Goldstein DA

更新日期：1988-02-01 00:00:00