MiR-451 inhibits proliferation of esophageal carcinoma cell line EC9706 by targeting CDKN2D and MAP3K1.

Abstract:

AIM:To investigate the underlying molecular mechanisms of miR-451 to inhibit proliferation of esophageal carcinoma cell line EC9706. METHODS:Assays for cell growth, apoptosis and invasion were used to evaluate the effects of miR-451 expression on EC cells. Luciferase reporter and Western blot assays were used to test whether cyclin-dependent kinase inhibitor 2D (CDKN2D) and MAP3K1 act as major targets of miR-451. RESULTS:The results showed that CDKN2D and MAP3K1 are direct targets of miR-451. CDKN2D and MAP3K1 overexpression reversed the effect of miR-451. MiR-451 inhibited the proliferation of EC9706 by targeting CDKN2D and MAP3K1. CONCLUSION:These findings suggest that miR-451 might be a novel prognostic biomarker and a potential target for the treatment of esophageal squamous cell carcinoma in the future.

journal_name

World J Gastroenterol

authors

Zang WQ,Yang X,Wang T,Wang YY,Du YW,Chen XN,Li M,Zhao GQ

doi

10.3748/wjg.v21.i19.5867

subject

Has Abstract

pub_date

2015-05-21 00:00:00

pages

5867-76

issue

19

eissn

1007-9327

issn

2219-2840

journal_volume

21

pub_type

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