Abstract:
:The complex of yeast cytochrome c peroxidase and cytochrome c is a paradigm of the biological electron transfer (ET). Building on seven decades of research, two different models have been proposed to explain its functional redox activity. One postulates that the intermolecular ET occurs only in the dominant, high-affinity protein-protein orientation, while the other posits formation of an additional, low-affinity complex, which is much more active than the dominant one. Unlike the high-affinity interaction-extensively studied by X-ray crystallography and NMR spectroscopy-until now the binding of cytochrome c to the low-affinity site has not been observed directly, but inferred mainly from kinetics experiments. Here we report the structure of this elusive, weak protein complex and show that it consists of a dominant, inactive bound species and an ensemble of minor, ET-competent protein-protein orientations, which summarily account for the experimentally determined value of the ET rate constant.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Van de Water K,Sterckx YG,Volkov ANdoi
10.1038/ncomms8073subject
Has Abstractpub_date
2015-05-06 00:00:00pages
7073issn
2041-1723pii
ncomms8073journal_volume
6pub_type
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