Early effects comparison of X-rays delivered at high-dose-rate pulses by a plasma focus device and at low dose rate on human tumour cells.

Abstract:

:A comparative study has been performed on the effects of high-dose-rate (DR) X-ray beams produced by a plasma focus device (PFMA-3), to exploit its potential medical applications (e.g. radiotherapy), and low-DR X-ray beams produced by a conventional source (XRT). Experiments have been performed at 0.5 and 2 Gy doses on a human glioblastoma cell line (T98G). Cell proliferation rate and potassium outward currents (IK) have been investigated by time lapse imaging and patch clamp recordings. The results showed that PFMA-3 irradiation has a greater capability to reduce the proliferation rate activity with respect to XRT, while it does not affect IK of T98G cells at any of the dose levels tested. XRT irradiation significantly reduces the mean IK amplitude of T98G cells only at 0.5 Gy. This work confirms that the DR, and therefore the source of radiation, is crucial for the planning and optimisation of radiotherapy applications.

journal_name

Radiat Prot Dosimetry

authors

Virelli A,Zironi I,Pasi F,Ceccolini E,Nano R,Facoetti A,Gavoçi E,Fiore MR,Rocchi F,Mostacci D,Cucchi G,Castellani G,Sumini M,Orecchia R

doi

10.1093/rpd/ncv163

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

383-7

issue

1-4

eissn

0144-8420

issn

1742-3406

pii

ncv163

journal_volume

166

pub_type

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