当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Effects of 5'-azacytidine and alendronate on a hepatocellular carcinoma cell line: a proteomics perspective.

Effects of 5'-azacytidine and alendronate on a hepatocellular carcinoma cell line: a proteomics perspective.

Abstract：

:Hepatocellular carcinoma (HCC) is the third leading cause of cancer related deaths around the world. Due to late diagnosis and development of drug resistance in patients suffering from HCC, development of more effective therapeutic strategies is inevitable. The aim of this study was to evaluate the combined apoptotic effect of 5'-Azacytidine (5'-AzaC) and alendronate (ALN) on Huh-7 HCC cell line and to explore differential expression at genomics and proteomics level. Incubation of HCC cell line with 5'-AzaC alone showed cell death in a time and dose dependent manner while in combination with ALN, increased cytotoxicity was observed. Up-regulation of CASP7(Caspase7) and LZTS1 (leucine zipper, putative tumor suppressor 1) and down-regulation of DNMT1(DNA (cytosine-5-)-methyltransferase 1) was noted in treated cells. Proteomic studies on the treated cells revealed altered expression of different proteins including peroxiredoxin 2 (Prx2), Annexin 5 (Anx5), Rho GTPase activating protein (RhoGAP), Nuclear factor-kappa B (NF-kB), tumor necrosis factor alpha-induced protein (TNF), triosephosphate isomerase (TPI), Glutathione S transferase (GSTP1) and Heat shock protein60 (HSP60). Our study demonstrated the cytotoxic effect of 5'-AzaC and ALN drug combination on Huh-7 HCC cells suggesting such combinations may be explored as a possible therapeutic approach. Current study revealed that Huh-7 HCC cells are sensitive to 5'-AzaC and ALN drug combination and such combination approaches could lead to the development of new therapeutic strategies. Furthermore, we also report the expression of Anx5 exclusively in untreated cancerous cell line indicating the possibility of being used as a potential therapeutic target and biomarker.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Ilyas A,Hashim Z,Zarina S

doi

10.1007/s11010-015-2395-1

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

53-61

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

405

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Typical phenotypic spectrum of velocardiofacial syndrome occurs independently of deletion size in chromosome 22q11.2.

    abstract：:Velocardiofacial syndrome (VCFS) is a relatively common developmental disorder characterized by craniofacial anomalies and conotruncal heart defects. Many VCFS patients present hemizygous deletions on part of chromosome 22q11.2; suggestive that haploinsufficiency in this region is responsible for this etiology. Most 2...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-007-9450-5

    authors： Sandrin-Garcia P,Abramides DV,Martelli LR,Ramos ES,Richieri-Costa A,Passos GA

    更新日期：2007-09-01 00:00:00

  • Protein phosphorylation in rat cardiac microsomes: effects of inhibitors of protein kinase A and of phosphatases.

    abstract：:The phosphorylation of rat cardiac microsomal proteins was investigated with special attention to the effects of okadaic acid (an inhibitor of protein phosphatases), inhibitor 2 of protein phosphatase 1 and inhibitor of cyclic AMP-dependent protein kinase (protein kinase A). The results showed that okadaic acid (5 mic...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006879427457

    authors： Sulakhe PV,Vo XT,Morris TE,Pato MD,Khandelwal RL

    更新日期：1997-10-01 00:00:00

  • Tyrosine hydroxylase regulation in the central nervous system.

    abstract：:Tyrosine hydroxylase is considered to be the rate-limiting enzyme in the synthesis of catecholamines in both the central and peripheral nervous system. Increased or decreased neuronal activity, stress, lesions, drug effects, endocrinological manipulations and experimental models of hypertension are associated with alt...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00225250

    authors： Masserano JM,Weiner N

    更新日期：1983-01-01 00:00:00

  • Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: involvement of glucose, insulin and calcium as stimulating factors.

    abstract：:The effect of refeeding on the expression of Ca(2+)-binding protein regucalcin mRNA in the liver of fasted rats was investigated. When rats were fasted overnight, the hepatic regucalcin mRNA level was reduced about 70% of that in feeding rats. Refeeding produced a remarkable elevation of hepatic regucalcin mRNA level ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00928911

    authors： Yamaguchi M,Oishi K,Isogai M

    更新日期：1995-01-12 00:00:00

  • Passive cigarette smoke and the renal glyoxalase system.

    abstract：:Cigarette smoking is associated with a number of fatal diseases, including cancer of different organs. A number of oxoaldehydes are found in cigarette smoke, among which methylglyoxal (MG) is known to cause toxicity to cells upon accumulation. In biological systems, MG is converted to s-d-lactoylglutathione by glyoxal...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1017992403656

    authors： Biswas S,Gairola CG,Das SK

    更新日期：2002-01-01 00:00:00

  • Role of reactive oxygen species in angiotensin II: induced receptor activator of nuclear factor-κB ligand expression in mouse osteoblastic cells.

    abstract：:Angiotensin II (Ang II) has been shown to induce receptor activator of nuclear factor-κB ligand (RANKL) expression in osteoblasts associated with its effect on reactive oxygen species (ROS) production. The objective of the present study was to investigate the potential pathways by which Ang II induces RANKL expression...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2160-x

    authors： Zhang Y,Zhang Y,Kou J,Wang C,Wang K

    更新日期：2014-11-01 00:00:00

  • Right ventricular collagen and fibronectin levels in patients with pulmonary atresia and ventricular septal defect.

    abstract：:Pulmonary atresia (PA) with ventricular septal defect (VSD) is an extreme form of tetralogy of Fallot with characteristic right ventricular hypertrophy. To reduce the right ventricular overload, these children have to undergo staged corrective surgery to restore physiological pulmonary perfusion. We studied the degree...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Peters TH,de Jong PL,Klompe L,Berger RM,Saxena PR,Sharma HS,Bogers AJ

    更新日期：2003-09-01 00:00:00

  • Epigenetic memory: gene writer, eraser and homocysteine.

    abstract：:Naturally chromatin remodeling is highly organized, consisting of histone acetylation (opening/relaxation of the compact chromatin structure), DNA methylation (inhibition of the gene expression activity) and sequence rearrangement by shifting. All this is essentially required for proper "in-printing and off-printing" ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-020-03895-4

    authors： Tyagi SC,Stanisic D,Singh M

    更新日期：2020-10-08 00:00:00

  • (Ca2+ + Mg2+) ATPase activity in kidney basolateral membrane in diabetes: role of atrial natriuretic peptide.

    abstract：:The (Ca2+ + Mg2+) ATPase which serves as a Ca2+ pump in the kidney basolateral membranes is essential to the maintenance of an intracellular Ca2+ concentration optimal for kidney function. Since atrial natriuretic peptide (ANP) is known to participate in the Ca2+ homeostasis mechanism, altered levels of ANP in diabete...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00230370

    authors： Sahai A,Ganguly PK

    更新日期：1991-06-26 00:00:00

  • Reduced activity of enzymes coupling ATP-generating with ATP-consuming processes in the failing myocardium.

    abstract：:Coupling of ATP-generating with ATP-consuming processes is an essential component in the cardiac bioenergetics responsible for optimal myocardial function. Although a number of enzymatic systems have been implicated in securing proper intracellular energy communication, their integrative response in a failing myocardi...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007016703229

    authors： Dzeja PP,Pucar D,Redfield MM,Burnett JC,Terzic A

    更新日期：1999-11-01 00:00:00

  • Decreased BMP2 signal in GIT1 knockout mice slows bone healing.

    abstract：:Endochondral ossification, an important stage of fracture healing, is regulated by a variety of signaling pathways. Transforming growth factor β (TGFβ) superfamily plays important roles and comprises TGFβs, bone morphogenetic proteins (BMPs), and growth differentiation factors. TGFβs primarily regulate cartilage forma...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2173-5

    authors： Sheu TJ,Zhou W,Fan J,Zhou H,Zuscik MJ,Xie C,Yin G,Berk BC

    更新日期：2014-12-01 00:00:00

  • OCT4 mutations in human erythroleukemic cells: implications for multiple drug resistance (MDR) phenotype.

    abstract：:The OCT4 transcription factor is a crucial stem cells marker and it has been related to the cancer stem cells concept. Moreover, it has also been associated to the multiple drug resistance (MDR) phenotype. Our first results pointed out a straight relation between OCT4 and ABC transporters in K562-derivative MDR (Lucen...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2260-7

    authors： Oliveira BR,Figueiredo MA,Trindade GS,Marins LF

    更新日期：2015-02-01 00:00:00

  • Cytotoxicity of glucoevatromonoside alone and in combination with chemotherapy drugs and their effects on Na+,K+-ATPase and ion channels on lung cancer cells.

    abstract：:Cardiac glycosides (CGs) are useful drugs to treat cardiac illnesses and have potent cytotoxic and anticancer effects in cultured cells and animal models. Their receptor is the Na+,K+ ATPase, but other plasma membrane proteins might bind CGs as well. Herein, we evaluated the short- and long-lasting cytotoxic effects o...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-04040-x

    authors： Schneider NFZ,Menegaz D,Dagostin ALA,Persich L,Rocha SC,Ramos ACP,Cortes VF,Fontes CFL,de Pádua RM,Munkert J,Kreis W,Braga FC,Barbosa LA,Silva FRMB,Simões CMO

    更新日期：2021-01-18 00:00:00

  • Clofibric acid or diethylmaleate supplemented diet decrease blood pressure in DOCA-salt treated male Sprague Dawley rats--relation with liver antioxidant status.

    abstract：:The effects of 8-week diethylmaleate (DEM) and clofibric acid (CFA) supplemented diet on blood pressure, body and liver weights, liver antioxidant status and nitric oxide synthase (NOS) activity were investigated in 8-week DOCA-salt treated and untreated Sprague-Dawley male rats. It appeared that DEM and particularly ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007112130769

    authors： Nicod L,Rodriguez S,Jacqueson A,Viollon-Abadie C,Berthelot A,Richert L

    更新日期：2000-10-01 00:00:00

  • Calcineurin is an important factor involved in glucose uptake in human adipocytes.

    abstract：:Calcineurin inhibitors are used in immunosuppressive therapy applied after transplantation, but they are associated with major metabolic side effects including the development of new onset diabetes. Previously, we have shown that the calcineurin inhibiting drugs tacrolimus and cyclosporin A reduce adipocyte and myocyt...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3261-0

    authors： Fonseca ACRG,Carvalho E,Eriksson JW,Pereira MJ

    更新日期：2018-08-01 00:00:00

  • Crosstalk between Noxa, Bcl-2, and ceramide in mediating p53-dependent apoptosis in Molt-4 human T-cell leukemia.

    abstract：:Ionizing radiation induces apoptosis in human Molt-4 leukemia cells in a p53-dependent manner. The tumor suppressor p53 stimulates various downstream targets that presumably trigger, individually or in concert, de novo ceramide synthesis and intrinsic apoptosis via mitochondrial outer membrane permeabilization (MOMP)....

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03874-9

    authors： Kobeissy H,Hage-Sleiman R,Dakdouk Z,Kozhaya L,Dbaibo G

    更新日期：2020-12-01 00:00:00

  • Induction of GLUT1 mRNA in response to azide and inhibition of protein synthesis.

    abstract：:Incubation of Clone 9 cells, a nontransformed rat liver cell line, in the presence of 5 mM azide results in an induction of GLUT1 mRNA which becomes detectable after approximately 3 h of continuous exposure to the agent. In examining the role of on-going protein synthesis in this response, we found that: (i) the induc...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006894527145

    authors： Behrooz A,Ismail-Beigi F

    更新日期：1998-10-01 00:00:00

  • Carnitine requirement of vascular endothelial and smooth muscle cells in imminent ischemia.

    abstract：:Vascular endothelial and -smooth muscle cells have been shown to use fatty acids as substrates for oxidative phosphorylation. Endothelial cells are more vulnerable to oxidative stress than muscle cells and are prone to loose carnitine early during hypoperfusion. This has been suggested by two observations. The first i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01270579

    authors： Hülsmann WC,Dubelaar ML

    更新日期：1992-10-21 00:00:00

  • Identification of an RNA aptamer binding hTERT-derived peptide and inhibiting telomerase activity in MCF7 cells.

    abstract：:Human telomerase reverse transcriptase is an essential rate-limiting component of telomerase complex. hTERT protein in association with other proteins and the human telomerase RNA (hTR) shows telomerase activity, essential for maintaining genomic integrity in proliferating cells. hTERT binds hTR through a decapeptide ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2907-7

    authors： Varshney A,Bala J,Santosh B,Bhaskar A,Kumar S,Yadava PK

    更新日期：2017-03-01 00:00:00

  • A role for guanidino compounds in the brain.

    abstract：:Guanidino compounds of guanidinoethanesulfonic acid, guanidinoacetic acid, guanidinosuccinic acid, N-acetylarginine, beta-guanidinopropionic acid, creatinine, gamma-guanidinobutyric acid, arginine, guanidine, methylguanidine, homoarginine and alpha-guanidinoglutaric acid are present in the mammalian brain. These guani...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： Hiramatsu M

    更新日期：2003-02-01 00:00:00

  • Carbon monoxide and bilirubin from heme oxygenase-1 suppresses reactive oxygen species generation and plasminogen activator inhibitor-1 induction.

    abstract：:Heme oxygenase-1 (HO-1) responds to a variety of oxidative stresses. We examined whether HO-1 expression influences pro-thrombotic processes, in which the involvement of oxidative stress has been reported. Since HO-1 knockout mice with a C57/BL6J background were not viable, embryonic cells from HO-1 deficient mice (E1...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9190-y

    authors： Matsumoto H,Ishikawa K,Itabe H,Maruyama Y

    更新日期：2006-10-01 00:00:00

  • Effects of fatty acid deficiency on the lipid composition and physical properties of guinea pig rough endoplasmic reticulum.

    abstract：:Twenty-six days of fat deficiency brought about a decrease of linoleic and an increase of oleic acid in rough endoplasmic reticulum (RER) of guinea pig liver. Arachidonic acid was only slightly decreased in some phospholipids whereas eicose-5,8,11-trienoic acid was not enhanced except in phosphatidyl-inositol. All the...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00229685

    authors： Soulages JL,Brenner RR

    更新日期：1987-12-01 00:00:00

  • Excitation-contraction coupling in heart muscle.

    abstract：:We have investigated the links between electrical excitation and contraction in mammalian heart muscle. Using isolated single cells from adult rat ventricle, a whole-cell voltage-clamp technique and quantitative fluorescence microscopy, we have measured simultaneously calcium current (ICa) and [Ca2+]i (with fura-2). W...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00220762

    authors： Lederer WJ,Cannell MB,Cohen NM,Berlin JR

    更新日期：1989-09-07 00:00:00

  • The effects of chronic administration of nandrolone decanoate on redox status in exercised rats.

    abstract：:For the past 40 years, anabolic-androgenic steroids have been used by a wide variety of athletes with the hope of improving their training, endurance, and performance. The aim of this study was to examine the chronic effects of nandrolone decanoate (20 mg/kg, s.c, Deca-Durabolin DECA(®)) on oxidative stress biomarkers...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-015-2571-3

    authors： Nikolic T,Zivkovic V,Jevdjevic M,Djuric M,Srejovic I,Djuric D,Jeremic N,Djuric D,Bolevich S,Jakovljevic V

    更新日期：2016-01-01 00:00:00

  • Spin trapping endogenous radicals in MC-1010 cells: evidence for hydroxyl radical and carbon-centered ascorbyl radical adducts.

    abstract：:Incubation of MC-1010 cells with the spin-trapping agent 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) followed by brief treatment with the solid oxidant lead dioxide (PbO2) yielded, after filtration, a cell-free solution that contained two nitroxyl adducts. The first was the hydroxyl radical adduct, 5,5-dimethyl-2-hydroxyp...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00928153

    authors： Bernofsky C,Bandara BM

    更新日期：1995-07-19 00:00:00

  • Identification of transcriptionally regulated genes in response to cellular iron availability in rat hippocampus.

    abstract：:The present study was attempted to identify transcriptionally regulated genes of the normal neurocytes responsive to iron availability. Postnatal rat hippocampus cells were primarily cultured either under the iron-loaded or depleted conditions. These cultured cells were applied for the generation of subtracted complem...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9377-2

    authors： Liu M,Xiao DS,Qian ZM

    更新日期：2007-06-01 00:00:00

  • The Ran-binding protein RanBPM can depress the NF-κB pathway by interacting with TRAF6.

    abstract：:Ran-binding protein in microtubule-organizing center (RanBPM) has been reported to interact with the neurotrophin receptors p75NTR and TrkA, meanwhile p75NTR and TrkA can also interact with TRAF6. Whether RanBPM interacts directly with TRAF6 has not yet been established. In this study, using a yeast two-hybrid system ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-1002-3

    authors： Wang L,Fu C,Cui Y,Xie Y,Yuan Y,Wang X,Chen H,Huang BR

    更新日期：2012-01-01 00:00:00

  • Environmental reprogramming of the expression of protein kinase CK2beta subunit in fish.

    abstract：:The dramatic segregation of the nucleolar components in winter-acclimatized carp is the most striking cellular-phenotypical feature observed during the seasonal adaptation of this fish toward the circannual changes in its habitat. Our studies also show that the carp habitat temperature and photoperiod winter condition...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Alvarez M,Kausel G,Figueroa J,Vera MI

    更新日期：2001-11-01 00:00:00

  • Antioxidant properties of dehydrozingerone and curcumin in rat brain homogenates.

    abstract：:The present study investigates the inhibition of lipid peroxidation by dehydrozingerone and curcumin in rat brain homogenates. Both the test compounds inhibited the formation of conjugated dienes and spontaneous lipid peroxidation. These compounds also inhibited lipid peroxidation induced by ferrous ions, ferric-ascor...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00928368

    authors： Rajakumar DV,Rao MN

    更新日期：1994-11-09 00:00:00

  • Neuroprotection against cobalt chloride-induced cell apoptosis of primary cultured cortical neurons by salidroside.

    abstract：:Salidroside, a phenol glycoside of plant origin, has been documented to possess a broad spectrum of pharmacological properties, including protective effects against neuronal death induced by different insults. To provide further insights into the neuroprotective functions peculiar to salidroside, this study used prima...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-0815-4

    authors： Zhang S,Chen X,Yang Y,Zhou X,Liu J,Ding F

    更新日期：2011-08-01 00:00:00