Abstract:
:Sonodynamic therapy (SDT) is a form of ultrasound therapy that has been shown to preferentially damage malignant cells based on the relatively enlarged size and altered cytology of neoplastic cells in comparison to normal cells. This study sought to determine whether cytoskeletal-directed agents that either disrupt (cytochalasin B and vincristine) or rigidify (jasplakinolide and paclitaxel) microfilaments and microtubules, respectively, affect ultrasonic sensitivity. U937 human monocytic leukemia cell populations were treated with each cytoskeletal-directed agent alone, and then sonicated at 23.5 kHz under relatively low power and intensity (20-40 W; 10-20 W/cm(2)), or at 20 kHz using moderate power and intensity (60 W; 80 W/cm(2)). In addition, human leukemia lines U937, THP1, K562, and Molt-4, and the murine leukemia line L1210 were sonicated using pulsed 20 kHz ultrasound (80.6 W; 107.5 W/cm(2)) both with and without the addition of cytoskeletal-directed agents to assess whether cytoskeletal-directed agents can potentiate ultrasonic sensitivity in different leukemia lines. Human hematopoietic stem cells (hHSCs) and leukocytes were sonicated with continuous 23.5 kHz ultrasound (20 W; 10 W/cm(2)) to determine whether this approach elicited the preferential damage of neoplastic cells over normal blood components. To determine whether ultrasonic sensitivity is exclusively dependent on cell size, leukemia cells were also enlarged via alteration of cell growth parameters including serum deprivation and re-addition, and plateau-phase subculturing. Results indicated that cytochalasin B/ultrasound treatments had the highest rates of initial U937 cell damage. The cells enlarged and partially synchronized, either by serum deprivation and re-addition or by plateau-phase subculturing and synchronous release, were not comparably sensitive to ultrasonic destruction based solely on their cell size. In addition, cytochalasin B significantly potentiated the ultrasonic sensitivity of all neoplastic cell lines, but not in normal blood cells, suggesting that preferential damage is attainable with this treatment protocol. Therefore, it is likely that ultrasonic cell lysis depends not only on cell size and type, but also on the specific molecular mechanisms used to induce cell enlargement and their effects on cell integrity. This is supported by the fact that either the microfilament-or microtubule-disrupting agent produced a higher rate of lysis for cells of a given size than the corresponding stabilizing agents.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Trendowski M,Wong V,Zoino JN,Christen TD,Gadeberg L,Sansky M,Fondy TPdoi
10.1016/j.canlet.2015.02.001subject
Has Abstractpub_date
2015-05-01 00:00:00pages
160-70issue
2eissn
0304-3835issn
1872-7980pii
S0304-3835(15)00092-0journal_volume
360pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::Our previous studies demonstrated that dietary garlic powder supplementation inhibits N-nitrosamine induced DNA alkylation in liver and mammary tissue. The present studies compared the impact of dietary supplementation with garlic powder or two garlic constituents, water-soluble S-allyl cysteine (SAC) and oil-soluble ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(96)04160-2
更新日期:1996-04-19 00:00:00
abstract::The incidence and pattern of ras oncogene mutations in human malignancies demonstrate geographic and racial differences. For example, specificity of alterations is found in cholangiocellular carcinomas in Thai patients with a different etiology from those in Japanese patients. In the present study, a comparison of ras...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00149-9
更新日期:1998-09-25 00:00:00
abstract::Metastasis is a characteristic and fatal feature of human malignancies. Its regulation is therefore of the utmost significance to clinicians. The present study was undertaken to determine whether a legume-derived protease inhibitor (PI) of trypsin/chymotrypsin, the field bean PI (FBPI), also has plasmin inhibitory act...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00090-1
更新日期:1998-07-03 00:00:00
abstract::TRIOBP isoforms 4 and 5 (TRIOBP-4/-5) are an actin-bundling protein associated with hearing loss. Here, we showed that TRIOBP-4/-5 was up-regulated in human pancreatic carcinoma cells. Knockdown of TRIOBP-4/-5 led to a loss of filopodia and a decrease in cell motility. Confocal microscopy showed that re-expression of ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2014.08.005
更新日期:2015-01-28 00:00:00
abstract::The development of multidrug resistance (MDR) to chemotherapy remains a major challenge in the treatment of cancer. Numerous mechanisms have been recognized that cause MDR, but one of the most important mechanisms is overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) transporters, through which the ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2018.01.021
更新日期:2018-05-01 00:00:00
abstract::We have previously shown that oligonucleotides designed to bind in triplex fashion to a specific p53 binding site homology inhibit the proliferation of colon cancer cells in vitro. The present study was designed to extend these observations in an in vivo model. HCT 116 human colon carcinoma cells were injected subcuta...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2004.02.002
更新日期:2004-06-08 00:00:00
abstract::Pancreatic cancer is currently one of the deadliest solid malignancies and pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer. In the past decade, diagnostics and surgical techniques for PDAC have been evolving steadily; however, clinical outcomes of patients with PDAC have shown litt...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2015.01.023
更新日期:2015-04-10 00:00:00
abstract::Acute myeloid leukemia (AML) cells in vivo are constantly exposed to lymphangiogenic cytokines such as VEGF-C. However, it is poorly understood how the VEGF-C signaling modulates the immune functions in the tumor microenvironment. We have previously reported that natural killer (NK) cells in AML patients strongly upre...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2014.08.027
更新日期:2014-11-28 00:00:00
abstract::Gelatinases play important roles in tumor progression and are abundantly expressed in a variety of malignant tumors. Antibody targeting gelatinases is a possible avenue to fight against cancer. However, antibody alone can not achieve curative efficacy. Herein, we demonstrated the intensified targeting therapy of a tan...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2010.02.020
更新日期:2010-09-01 00:00:00
abstract::These studies examined the impact of dietary corn oil and its major constituent fatty acids on the occurrence of 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary DNA adducts. In study 1, rats were fed diets containing 5, 10 or 20% corn oil for 2 weeks prior to DMBA treatment (25 mg/kg). Mammary DNA adducts increa...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(96)04323-6
更新日期:1996-09-10 00:00:00
abstract::A single tumorigenic dose of methylazoxymethanol acetate increased adenylate cyclase activity in total liver homogenates 70-100% by the seventh day after treatment. The increased activity occurred in the plasma membranes rather than in the nuclei and was accompanied by a significant increase in 5'-nucleotidase activit...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(75)95764-x
更新日期:1975-11-01 00:00:00
abstract::To estimate the contents of flavonoids in the Danish diet, we have used a high performance liquid chromatographic method for the analysis of fruits, vegetables and beverages. The flavonoid contents were quantified, and the observations were verified by mass spectrometry. The investigation is not completed, but we are ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(97)04651-x
更新日期:1997-03-19 00:00:00
abstract::As the frequent emergency of resistant tumor cells during treatment, the development of new agents with new modes of action attracts a great deal of interest. Polybia-MPI was a short cationic alpha-helical amphiphilic peptide that has selective toxicity toward cancer cells but no hemolytic activity. Its target selecti...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.12.027
更新日期:2009-06-08 00:00:00
abstract::Retinoic acid and retinol induced functional and morphological differentiation of human promyelocytic leukemia cells (HL-60) into mature granulocytes, but did not induce functional or morphological differentiation of mouse myeloid leukemia cells (M1). Cellular retinoic acid-binding protein, but not retinol-binding pr...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(81)90079-3
更新日期:1981-06-01 00:00:00
abstract::We investigated the influence of TNF-alpha on the metastasis of cancer cells. Treatment of cultured colon 26 cells with TNF-alpha enhanced metastatic properties including production of MMP-9, adhesion, migration and invasion. Cells treated with TNF-alpha in vitro showed marked potential to metastasize to the lung and ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2004.12.027
更新日期:2005-12-08 00:00:00
abstract::The influence of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone, a mutagen in chlorinated water, on cell proliferation and lipid peroxidation in the glandular stomach mucosa was investigated in male 4-week-old Wistar rats. Animals were given 50 p.p.m., 25 p.p.m., 12.5 p.p.m., 6.25 p.p.m. or 0 p.p.m. of MX soluti...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(94)90268-2
更新日期:1994-10-14 00:00:00
abstract::The fruiting body of Antrodia camphorata is well known in Taiwan as a traditional medicine for treating cancer and inflammation. The purpose of this study was to evaluate the apoptotic effects of ethylacetate extract from A. camphorata (EAC) fruiting bodies in two human liver cancer cell lines, Hep G2 and PLC/PRF/5. T...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2004.08.012
更新日期:2005-04-18 00:00:00
abstract::Patients (459) with chronic gastritis, ulcers, polyposis and different types of gastric cancers were examined by endoscopic fluorescent technique using a special drug based on the sodium salt of fluorescein (F1). Eighty-five percent of gastric cancers showed positive fluorescence. The F1 distribution in the stomach of...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(93)90163-4
更新日期:1993-04-30 00:00:00
abstract::gamma-Glutamyltranspeptidase (gamma-GTP) activity was found in a human pancreatic cancer cell line, HPC-Y1, cultivated in a serum-free chemically defined medium. The gamma-GTP stained in the cytoplasms as fine granules and was produced constantly in a protein-free chemically defined medium. The detergent- or protease-...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(84)80037-3
更新日期:1984-12-01 00:00:00
abstract::Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections (STIs) in young women. They can occur in one or multiple areas of the female genitalia. Usually, the vulva is the initial site of implantation for HPV. The purpose of our study is to evaluate the epidemiological aspects, inc...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(03)00218-0
更新日期:2003-07-10 00:00:00
abstract::Aberrant expression of many long non-coding RNAs has been observed in various types of cancer, implicating their crucial roles in tumorigenesis and cancer progression. Emerging knowledge with regard to the critical physiological and pathological roles of long non-coding RNAs in cancers makes them potential targets in ...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2018.01.042
更新日期:2018-04-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. Many kinases have been found to be intimately involved in oncogenesis and the deregulation of kinase function has emerged as a major mechanism by which cancer cells evade normal physiological constraints on growth and surviv...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.09.017
更新日期:2016-12-01 00:00:00
abstract::Gastric cancers with and those without high-frequency microsatellite instability (MSI-H) represent distinctive pathways of carcinogenesis. The aim of this study was to clarify if expression of p53 related genes involved in angiogenesis is differentially regulated between these cancers. We systematically analyzed the e...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2007.02.004
更新日期:2007-08-28 00:00:00
abstract::V600E being the most common mutation in BRAF, leads to constitutive activation of the MAPK signaling pathway. The majority of V600E BRAF positive melanoma patients treated with the BRAF inhibitor vemurafenib showed initial good clinical responses but relapsed due to acquired resistance to the drug. The aim of the pres...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.02.041
更新日期:2015-05-28 00:00:00
abstract::Fumonisin B1 (FB1), a mycotoxin produced by a common corn contaminant Fusarium moniliforme and a hepatocarcinogen in rats, has been previously suggested to act as a poor initiator, but a better promoter of gamma-glutamyltranspeptidase (GGT)-positive rat liver preneoplastic lesions. Using glutathione S-transferase-plac...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00046-9
更新日期:1998-06-05 00:00:00
abstract::Standard chemotherapy for pancreatic ductal adenocarcinoma (PDAC), nab-paclitaxel (NPT) plus gemcitabine (Gem), has led to an average survival of 8.5 months. Presently, no therapeutics exist that effectively target the KRAS oncogene, activated in 95% of PDACs, but alternative strategies focus on inhibition of downstre...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.05.037
更新日期:2019-09-10 00:00:00
abstract::Mesenchymal stem cells (MSCs) are the first type of stem cells to be utilized in clinical regenerative medicine, mainly owing to their capacity for multipotent differentiation and the feasibility of autologous transplantation. More recently, the specific tumor-oriented migration and incorporation of MSCs have been dem...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2011.02.012
更新日期:2011-06-01 00:00:00
abstract::Recent clinical studies have indicated that intra-tumoral gene expression levels of 5-fluorouracil (5-FU) metabolism-related enzymes may predict the clinical response of several cancers to 5-FU-based chemotherapy. However, few studies examining oral squamous cell carcinomas (OSCCs) have been reported. In this study, w...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.09.010
更新日期:2009-02-18 00:00:00
abstract::Transforming growth factors (TGFs) are implicated in malignancy, therefore qualitative and quantitative differences of these growth factors in chronic myelogenous leukemia (CML) in chronic phase has been investigated in this report. Induction of anchorage independent growth of BALB/c 3T3 and NRK-49F fibroblasts was us...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(86)90181-3
更新日期:1986-09-01 00:00:00
abstract:INTRODUCTION:Glioma initiating cells (GICs) play important roles in tumor initiation and progression. However, interactions between tumor cells and host cells of local tumor microenvironment are kept largely unknown. Besides GICs and their progeny cells, whether adjacent normal glial cells contribute to tumorigenesis d...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.05.026
更新日期:2015-09-01 00:00:00