Abstract：
OBJECTIVE:To develop a technique with the potential of isolating genetically fit sperm for assisted reproductive technology (ART) treatment without compromising its structural or functional competence. DESIGN:Observational study. SETTING:University hospital. PATIENT(S):Fifty patients undergoing infertility diagnosis and 88 couples undergoing ART treatment. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Under an electric field, the percentage of positively charged sperm (PCS), negatively charged sperm (NCS), and neutrally charged sperm was determined for each ejaculate before and after density gradient centrifugation (DGC), and evaluated for sperm DNA damage, histone retention, and couples' ART outcomes. Subsequently, PCS, NCS, and neutrally charged sperm were selected using an intracytoplasmic sperm injection needle and directly analyzed for DNA damage. RESULT(S):There was a reduction in the NCS population (95.10% ± 0.94% vs. 54.48% ± 2.39%) and an increase in the PCS population (4.28% ± 0.58% vs. 42.52% ± 2.36%) after DGC. The DNA damage was inversely proportional to %NCS (r(2) = -0.242) and directly proportional to the %PCS (r(2) = 0.206). When sperm were picked according to their charge and directly analyzed, sperm DNA damage was lower in the NCS population (3.9% ± 1.5%) compared with control (17.3% ± 3.2%) and %PCS populations (27.8% ± 6.0%). The %NCS was positively associated with fertilization rate (r(2) = 0.469) and blastocyst development (r(2) = 0.308) and inversely associated with embryo arrest (r(2) = -0.253). Implantation rate and clinical pregnancies were higher in patient groups with increased NCS. CONCLUSION(S):Selection of NCS through micro-electrophoresis has the potential to isolate sperm relatively free of DNA damage to be used in ART.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Simon L,Murphy K,Aston KI,Emery BR,Hotaling JM,Carrell DTdoi
10.1016/j.fertnstert.2014.10.047subject
Has Abstractpub_date
2015-02-01 00:00:00pages
361-6.e3issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(14)02307-3journal_volume
103pub_type
杂志文章abstract：:This report is an analysis of 16 cycles in 15 patients stimulated with human menopausal gonadotropin and clomiphene citrate for the purpose of follicular recruitment in an in vitro fertilization program, all of which resulted in endogenous luteinizing hormone (LH) surges. A study of the pattern of estradiol (E2) respo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49584-1
更新日期：1986-09-01 00:00:00
abstract：OBJECTIVE:To study the psychological effects of multifetal pregnancy reduction. DESIGN AND SETTING:Hour-long semistructured telephone interviews with both multifetal pregnancy reduction patients and control subjects. PATIENTS:Forty-two multifetal pregnancy reduction patients were contacted within 1 year of undergoing...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：OBJECTIVE:To investigate the serum pattern of free leptin, bound leptin, and soluble leptin receptor throughout the physiological menstrual cycle. DESIGN:Prospective observational study. SETTING:Tertiary care center for gynecological endocrinology and reproductive medicine and a university research laboratory. PATIE...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.06.020
更新日期：2004-02-01 00:00:00
abstract：OBJECTIVE:To examine the effect of supraphysiologic doses of testosterone (T) on plasma total homocysteine (tHcy) concentrations in patients with Klinefelter's syndrome (KS). DESIGN:Prospective clinical study. SETTING:University hospital. PATIENT(S):Thirty-two newly diagnosed male patients with KS, and 20 healthy, v...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.11.029
更新日期：2004-05-01 00:00:00
abstract：OBJECTIVE:To identify the gene(s) involved in the etiology of premature ovarian insufficiency in a highly consanguineous Tunisian family. DESIGN:Genetic analysis of a large consanguineous family with several affected siblings. SETTING:University hospital-based cytogenetics and molecular genetics laboratories. PATIEN...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2017.07.015
更新日期：2017-10-01 00:00:00
abstract：:Pulsatile administration of human menopausal gonadotropin (hMG) via the subcutaneous route was evaluated in 15 patients with various ovulatory disorders. Administration of hMG was started at a dose of 4.6875 IU (75 IU/day) or 9.375 IU (150 IU/day) per pulse every 90 minutes. Ovulation was observed in 26 (92.9%) of 28 ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49456-2
更新日期：1986-07-01 00:00:00
abstract：OBJECTIVE:To determine the effects of low-dose aspirin on ovarian response, uterine and ovarian blood flow velocity, and implantation and pregnancy rates in patients undergoing IVF. DESIGN:Prospective, randomized, double-blind placebo-controlled assay. SETTING:Department of Reproductive Medicine, CER Medical Institut...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(99)00088-6
更新日期：1999-05-01 00:00:00
abstract：:Throughout the world, adults with HIV are living longer, and many are assessing their options for reproduction. The growing body of scientific evidence and commentary concerning the outcomes of infertility services provided to these adults demands systematic summary and long-term surveillance if safety, quality, and b...
journal_title：Fertility and sterility
pub_type： 评论,杂志文章
doi：10.1016/j.fertnstert.2005.07.1325
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:To compare pregnancy rates after fallopian tubal and uterine transfer of cryopreserved embryos. DESIGN:Prospective randomized trial with assignment to treatment groups by a random number table. SETTING:University of Iowa Hospitals and Clinics, a tertiary care academic institution. PATIENTS:Forty patients w...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)57429-9
更新日期：1995-03-01 00:00:00
abstract：:The antiprogesterone compound RU 486 (17 beta-hydroxy-11 beta-[4-dimethylaminophenyl]-17 alpha-[ 1-propynyl]estra-4,9-dien-3-one; Roussel-Uclaf, Paris, France) was used to induce menstruation in a randomized controlled trial. Castrated adult female cynomolgus monkeys (n = 17) received sequential subcutaneous Silastic ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47246-8
更新日期：1983-08-01 00:00:00
abstract：OBJECTIVE:To study the changes in semen parameters, gonadotropic and sex hormones, and serum leptin in obese fertile and infertile oligozoospermic men. DESIGN:Prospective. SETTING:University hospital. PATIENT(S):Forty-two obese fertile and 80 obese infertile oligozoospermic men (body mass index [BMI] >30). INTERVEN...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.03.085
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVE:To demonstrate three approaches to uterine artery occlusion at time of myomectomy as a blood-sparing intraoperative technique. DESIGN:A step-by-step explanation of the procedure with surgical video footage. SETTING:Academic medical center. PATIENT(S):Patients undergoing laparoscopic myomectomy, for whom a ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2019.01.019
更新日期：2019-05-01 00:00:00
abstract：:Activities of deoxyribonucleic acid (DNA) polymerase alpha, beta, and gamma were measured in extracts of testicular biopsy specimen obtained from 37 cases of male infertility with left varicocele and compared with those of 6 normal controls. It was observed that levels of DNA polymerase alpha, beta, and gamma were sig...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60318-7
更新日期：1988-11-01 00:00:00
abstract：:A case of EP after GIFT into the contralateral tube is presented. Hydrotubation with Earle's medium immediately before the GIFT cannot be excluded as a possible cause of gamete migration. The complete demise of the CL also demonstrates that trophoblast can implant and develop without steroidal support. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54925-5
更新日期：1992-03-01 00:00:00
abstract：OBJECTIVE:To examine the relationship of ovulation-inducing drugs and ovarian cancer. DESIGN:Retrospective cohort study, with additional follow-up since initial report. SETTING:Five large reproductive endocrinology practices. PATIENT(S):In a retrospective cohort of 9,825 women evaluated for infertility at five clini...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2013.08.008
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVE:To determine the distribution of catalase in eutopic and ectopic endometria in patients with endometriosis or adenomyosis. DESIGN:Retrospective randomized study. SETTING:Department of obstetrics and gynecology in a university hospital. PATIENT(S):Thirty-three patients with endometriosis, 36 with adenomyosi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03344-7
更新日期：2002-10-01 00:00:00
abstract：OBJECTIVE:Since little is known about the actual incidence and fate of vacuoles at different stages of development this preliminary study was set up to accurately measure vacuoles and track them to day 5. DESIGN:Prospective study. SETTING:Women's General Hospital in Austria. PATIENT(S):A total of 223 consecutive IVF...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.02.009
更新日期：2005-06-01 00:00:00
abstract：:Most oocyte donation programs have experienced higher pregnancy rates than usually seen in regular in vitro fertilization (IVF), suggesting that the quality of either the oocytes or the endometrium is superior. To clarify this issue we analyzed the results of transfers of 136 cryopreserved embryos originating either f...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53830-8
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVE:To estimate the incidence of endometriosis in an operative cohort of women seeking clinical care and in a matched population cohort to delineate more fully the scope and magnitude of endometriosis in the context of and beyond clinical care. DESIGN:Matched-exposure cohort design. SETTING:Surgical centers in ...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2011.05.087
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To determine whether antisperm autoantibody production after prepubertal vas injury is influenced by immediate repair of the vas compared to delay of the reanastomosis until sexual maturity. DESIGN:Animal study comparing early repair, late repair, and sham-operated groups. SETTING:Research laboratory in a m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00501-4
更新日期：2000-02-01 00:00:00
abstract：:The addition of 4% dl-methionine to a diet of 18% or 12% casein reduced pregnancy maintenance in the rat from 100% to 48% and 5%, respectively. Fetal weight was subnormal. The major influence of methionine was not due to reduced food intake as determined by pair-fed controls but was related to reduced ovarian function...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42563-x
更新日期：1977-05-01 00:00:00
abstract：:In vitro fertilization and embryo transfer (IVF-ET) and gamete intrafallopian transfer (GIFT) are used to treat intractable infertility in women with no distortion of the pelvic viscera, despite the lack of controlled trials demonstrating efficacy. The mechanism of any purportedly enhanced cycle fecundity in ovulatory...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59414-x
更新日期：1987-09-01 00:00:00
abstract：:This paper reports further observations on our previous study of 203 Copper 7 intrauterine insertions performed in 199 women. There have now been accumulated 3799 woman-months of use. At least a 2-year period of observation has been attained from first insertion until these data were analyzed. The Copper 7 intrauterin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
abstract：:The assessment of anti-phospholipid antibodies (APA) is not indicated among couples undergoing IVF, and therapy is not justified on the basis of existing data. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2008.08.100
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the effects of a prolonged duration of gonadotropin-releasing hormone agonist (GnRH-a) in pituitary down-regulation for controlled ovarian hyperstimulation (COH) on the live-birth rate in nonendometriotic women undergoing in vitro fertilization and embryo transfer (IVF-ET). DESIGN:Retrospective c...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.03.030
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:To compare pregnancy rates (PRs) using blastocysts cryopreserved on day 7 with those cryopreserved on days 5 and 6. DESIGN:Retrospective observational cohort study. SETTING:Infertility center performing IVF. PATIENT(S):Eight hundred women with infertility undergoing frozen ET. INTERVENTION(S):Blastocysts ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.06.021
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVE:To investigate whether the antiprogestin RU486 acts primarily on the hypothalamus to delay the midcycle gonadotropin surge and thus gain insight into the site(s) of action of P in the control of ovulation. DESIGN:Prospective, crossover, single-blinded clinical study. SETTING:Outpatient clinic in an academic...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)56811-3
更新日期：1994-07-01 00:00:00
abstract：:Adult beagle bitches received 0.2 to 75 mg/kg doses of medroxyprogesterone acetate (MPA) once every 3 months for 1 to 1.5 years. Doses greater than 2.0 mg/kg prevented the recurrence of ovarian cycles; lower doses did not. MPA (10 and 75 mg/kg) induced a dose-related development of multiple, large (greater than 5 mm d...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：OBJECTIVE:To characterize the variability of hormonal profiles during the luteal phase in normal cycles. DESIGN:Observational study. SETTING:Not applicable. PATIENT(S):Ninety-nine women contributing 266 menstrual cycles. INTERVENTION(S):The women collected first morning urine samples that were analyzed for estrone-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2017.05.012
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the immunoactivity of human follicular fluids (FFs) of ovaries stimulated with exogenous gonadotropins. PARTICIPANTS:Follicular fluids and peripheral blood mononuclear cells (PBMCs) were obtained at the time of ovum pick-up from 15 women (25 to 34 years of age) undergoing gonadotropin stimulation...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56886-1
更新日期：1994-08-01 00:00:00
