Identification of coexistence of DNA methylation and H3K27me3 specifically in cancer cells as a promising target for epigenetic therapy.


:Alterations of epigenetic modifications are promising targets for cancer therapy, and several epigenetic drugs are now being clinically utilized. At the same time, individual epigenetic modifications have physiological functions in normal cells, and cancer cell specificity is considered difficult to achieve using a drug against a single epigenetic modification. To overcome this limitation, a combination of epigenetic modifications specifically or preferentially present in cancer cells is a candidate target. In this study, we aimed to demonstrate (i) the presence of a cancer cell-specific combination of epigenetic modifications by focusing on DNA methylation and trimethylation of histone H3 lysine 27 (H3K27me3) and (ii) the therapeutic efficacy of a combination of DNA demethylation and EZH2 inhibition. Analyses of DNA methylation and H3K27me3 in human colon, breast and prostate cancer cell lines revealed that 24.7±4.1% of DNA methylated genes had both DNA methylation and H3K27me3 (dual modification) in cancer cells, while it was 11.8±7.1% in normal cells. Combined treatment with a DNA demethylating agent, 5-aza-2'-deoxycytidine (5-aza-dC) and an EZH2 inhibitor, GSK126, induced marked re-expression of genes with the dual modification, including known tumor-suppressor genes such as IGFBP7 and SFRP1, and showed an additive inhibitory effect on growth of cancer cells in vitro. Finally, an in vivo combined treatment with 5-aza-dC and GSK126 inhibited growth of xenograft tumors more efficiently than a single treatment with 5-aza-dC. These results showed that the dual modification exists specifically in cancer cells and is a promising target for cancer cell-specific epigenetic therapy.






Takeshima H,Wakabayashi M,Hattori N,Yamashita S,Ushijima T




Has Abstract


2015-02-01 00:00:00














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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: McDonald TA,Waidyanatha S,Rappaport SM

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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  • Generation of S phase-dependent DNA double-strand breaks by Cr(VI) exposure: involvement of ATM in Cr(VI) induction of gamma-H2AX.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


    authors: Murphy ME

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    pub_type: 杂志文章


    authors: Dragan YP,Campbell HA,Baker K,Vaughan J,Mass M,Pitot HC

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    pub_type: 杂志文章


    authors: Awasthi N,Zhang C,Schwarz AM,Hinz S,Wang C,Williams NS,Schwarz MA,Schwarz RE

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    pub_type: 杂志文章


    authors: Papanikolaou A,Wang QS,Papanikolaou D,Whiteley HE,Rosenberg DW

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    pub_type: 杂志文章


    authors: van den Goorbergh JA,de Wit H,Tijdens RB,Mulder GJ,Meerman JH

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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