Abstract:
:Specific repair endonucleases were used to quantify oxidative modifications in mitochondrial DNA (mtDNA) from rat liver and from porcine liver and kidney by means of a relaxation assay. In rat liver mitochondria the number of modifications sensitive to formamidopyrimidine--DNA glycosylase (FPG protein), which include 8-hydroxyguanine (8-oxo-7,8-dihydroguanine) residues, was only 0.8 +/- 0.2 per 10(5) base pairs (bp). Even lower values were observed in porcine kidney (0.5 +/- 0.3 per 10(5) bp) and liver (0.4 +/- 0.2 per 10(5) bp). The numbers of sites of base loss (AP sites) sensitive to T4 endonuclease V and of 5,6-dihydropyrimidines sensitive to endonuclease III were less than 0.2 per 10(5) bp in all cases. The data provide evidence that the steady-state levels of oxidative mtDNA modifications are low under physiological conditions, either because reactive oxygen species generated in the mitochondria are instantly inactivated or because of efficient DNA repair processes inside mitochondria.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Hegler J,Bittner D,Boiteux S,Epe Bdoi
10.1093/carcin/14.11.2309subject
Has Abstractpub_date
1993-11-01 00:00:00pages
2309-12issue
11eissn
0143-3334issn
1460-2180journal_volume
14pub_type
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