Abstract:
:We have compared liver, kidney and colon DNA damage, as single strand breaks, in mice with different strain-dependent susceptibility to the colon-specific carcinogen 1,2-dimethylhydrazine (DMH). The mouse strains studied were: AKR/J, DBA2 totally resistant; CD1, C57BL/6N moderately susceptible; SWR/J very susceptible to DMH-induced carcinogenesis. DNA breaks were estimated from the elution rate constant (K) according to the alkaline elution technique. At 4 h after carcinogen administration a substantial and comparable DNA damage was found in liver and kidney in all the strains examined. The DNA fragmentation index, however, reached a maximum value at 2 h after treatment in the liver of the most susceptible strain (SWR/J). About 50% of the liver DNA damage detected in all five strains 4 h after DMH administration persisted at 24 h after treatment and was totally repaired at 72 h. Kidney DNA damage decreased in 48 h toward the range of control values. In colon epithelial cells (the carcinogen target tissue) 2 and 4 h after DMH administration the amount of DNA single strand breaks was correlatable with the strain sensitivity to the carcinogen. In the time interval studied (2-72 h after DMH administration) the decrease of colon DNA damage was linear in the resistant strains. In contrast, in the more susceptible strain (SWR/J), the amount of DNA breaks remained high up to 24 h after treatment and returned to background level at 72 h.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Bolognesi C,Mariani MR,Boffa LCdoi
10.1093/carcin/9.8.1347subject
Has Abstractpub_date
1988-08-01 00:00:00pages
1347-50issue
8eissn
0143-3334issn
1460-2180journal_volume
9pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Despite decades of research in defining the health effects of low-dose (<100 mGy) ionizing photon radiation (LDR), the relationship between LDR and human cancer risk remains elusive. Because chemical carcinogens modify the tumor microenvironment, which is critical for cancer development, we investigated the role and ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs159
更新日期:2012-07-01 00:00:00
abstract::Specific repair endonucleases were used to quantify oxidative modifications in mitochondrial DNA (mtDNA) from rat liver and from porcine liver and kidney by means of a relaxation assay. In rat liver mitochondria the number of modifications sensitive to formamidopyrimidine--DNA glycosylase (FPG protein), which include ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.11.2309
更新日期:1993-11-01 00:00:00
abstract::The Notch pathway plays a key role in the development and is increasingly recognized for its importance in cancer. We demonstrated previously the overexpression of Notch-1 and its ligands in gliomas and showed that their knockdown inhibits glioma cell proliferation and survival. To elucidate the mechanisms downstream ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn079
更新日期:2008-05-01 00:00:00
abstract::Cancer incidence and mortality rates show great variations across nations and between population groups. These variations are largely explained by differences in age distribution, diet and lifestyle, access to health care, cultural barriers and exposure to carcinogens and pathogens. Cancers caused by infections are si...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgr066
更新日期:2011-08-01 00:00:00
abstract::Reactive intermediates (ultimate mutagens/carcinogens) generated by alkylating agents are unstable and difficult to characterize by chemical means. We have used a genetic system to distinguish the in vivo interactions of eight carcinogenic methylating agents and five ethylating agents by the patterns of induced mutati...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.7.1161
更新日期:1991-07-01 00:00:00
abstract::Despite their individual key roles in promoting head and neck squamous cell carcinoma (HNSCC) progression and treatment resistance, little is known about the impact of intratumoral hypoxia on the activity of the epidermal growth factor receptor (EGFR) signaling pathway in this cancer type. Here, we show that in highly...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq078
更新日期:2010-07-01 00:00:00
abstract::The ability of sodium bisulfite to induce morphological transformation and mutagenesis of cultured Syrian hamster embryo cells was examined. Treatment of the cells at neutral pH for 15 min with 5-20 mM sodium bisulfite resulted in a dose-dependent increase in cell transformation but no induction of gene mutations meas...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.10.1869
更新日期:1990-10-01 00:00:00
abstract::We conducted a cross-sectional molecular epidemiological study of coke oven workers exposed to the established carcinogen polycyclic aromatic hydrocarbons (PAHs) to evaluate the relationships between both traditional 'exposure markers' and a series of biomarkers, including urinary 1-hydroxypyrene as a marker of intern...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.11.1963
更新日期:1998-11-01 00:00:00
abstract::Benzoquinones (BQ) are genotoxic species that stem from metabolism of phenolic compounds. We have developed a method for measuring adducts of 1,2- and 1,4-benzoquinone (1,2-BQ and 1,4-BQ) with cysteine residues of hemoglobin (Hb) and albumin (Alb). The method employs a reductive catalyst (Raney nickel) to selectively ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.9.1927
更新日期:1993-09-01 00:00:00
abstract::O(6)-alkylguanine-DNA alkyltransferase (AGT) is a suicide protein that corrects DNA damage by alkylating agents and may also serve to activate environmental carcinogens. We expressed human wild-type and two active mutant AGTs in bacteria that lack endogenous AGT and are also defective in nucleotide excision repair, to...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.11.2089
更新日期:1999-11-01 00:00:00
abstract::Previous studies have shown that polycyclic aromatic hydrocarbons (PAHs) mobilize intracellular Ca2+ in human T cells by inositol trisphosphate-dependent mechanisms resulting from activation of phospholipase C-gamma by SRC-related protein tyrosine kinases, thereby mimicking antigen-receptor activation. Ca2+ appears to...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.6.1177
更新日期:1997-06-01 00:00:00
abstract::Ovarian surface epithelium (OSE) is considered to give rise to epithelial ovarian carcinomas (EOCs). To elucidate early processes contributing to the development of EOCs from the OSE, two batches of primary human OSE cells were transduced with non-viral human genes (mutant Cdk4, cyclinD1 and hTERT) so as to efficientl...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp007
更新日期:2009-03-01 00:00:00
abstract::N-nitrosopiperidine (NPIP) is a potent esophageal carcinogen in rats whereas structurally similar N-nitrosopyrrolidine (NPYR) induces liver, but not esophageal tumors. NPIP is a possible causative agent for human esophageal cancer. Our goal is to explain mechanistically these differing carcinogenic activities in the e...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.2.291
更新日期:2003-02-01 00:00:00
abstract::Aromatic DNA adducts in total white blood cells, cytochrome P450 (CYP) class 1A1 and glutathione transferase (GST1) class mu genotypes and micronuclei in T- and B-lymphocytes were studied in 69 full-time chimney sweeps and 35 controls, all male subjects. The sweeps had a higher (22%) but statistically non-significant ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.7.1347
更新日期:1994-07-01 00:00:00
abstract::Genetically modified mice with overexpressed and/or deleted genes have been used extensively to model human cancer. However, it is uncertain as to what extent the mouse models reproduce the corresponding cancers in humans. We have compared the global gene expression patterns in human and mouse hepatocellular carcinoma...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi030
更新日期:2005-06-01 00:00:00
abstract::The aim of the present study was to investigate the effects of galacto-oligosaccharides (GOS, Elix'or) on the development of aberrant crypt foci (ACF) and colorectal tumours in rats treated with azoxymethane (AOM). Two groups of 102 male Fischer 344 rats were injected twice with AOM to induce colorectal tumours, and f...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.1.127
更新日期:2001-01-01 00:00:00
abstract::The breast cancer gene BRCA1 has previously been cloned from both human and mouse. We cloned a fragment of the rat Brca1 homologue in order to map it and explore its biological function. Partial cDNA fragments of the rat Brca1 homologue were isolated by RT-PCR. Sequence analysis revealed that the RING-finger domain is...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.8.1561
更新日期:1996-08-01 00:00:00
abstract::To indicate how readily nitrosamines (NAms) diffuse into the esophagus, we measured diffusion rate (flux) through rat esophagus of dialkyl-NAms using side-by-side diffusion apparatuses. Mucosal and serosal flux at 37 degrees C of two NAms, each at 50 microM, was followed for 90 min by gas chromatography-thermal energy...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.5.825
更新日期:1999-05-01 00:00:00
abstract::4-Oxo-4-(3-pyridyl)butanal (OPB) is an aldehyde formed during the activation of the tobacco-specific N-nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Using the DNA alkaline elution technique, the properties of DNA alkali-labile sites induced in the isolated rat hepatocytes by NNK and OPB were compar...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.11.2135
更新日期:1991-11-01 00:00:00
abstract::A model of oral mucosal carcinogenesis using the water-soluble carcinogen 4-nitroquinoline-1-oxide (4NQO) was combined with a model of oral mucosal candidosis to examine the ability of Candida albicans to promote the development of neoplasia in suitably initiated epithelium. Sprague-Dawley rats were initiated by the a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.5.783
更新日期:1992-05-01 00:00:00
abstract::The haloethylnitrosoureas form a cytotoxic DNA cross-link in a series of reactions which involves initial alkylation of the O6 position of guanine and rearrangement to the intermediate, 1,O6-ethanoguanine; 1,O6-ethanoguanine then reacts with a neighboring cytosine base. O6-Alkylguanine-DNA alkyltransferase can interru...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.2.195
更新日期:1993-02-01 00:00:00
abstract::The expression of A and P forms of glutathione S-transferase (GST-A and P), two cytochrome P-450 isoenzymes (P-450 PB3a and P-450 MC2), microsomal epoxide hydrolase (mEHb), glucose-6-phosphate dehydrogenase (G6PD) and gamma-glutamyltranspeptidase (gamma-GT) was compared in preneoplastic liver lesions and background pa...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.4.547
更新日期:1988-04-01 00:00:00
abstract::Apigenin, a flavonoid abundant in various vegetables and fruits, including parsley and onions, has been reported to possess anticarcinogenic effects. However, the direct molecular target of apigenin and its chemopreventive effect on ultraviolet (UV)-induced skin inflammation are not understood fully. Herein, we examin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs358
更新日期:2013-02-01 00:00:00
abstract::It is widely believed that abnormal production of polypeptide growth factors, together with other molecular alterations, play an important role in neoplastic development. Transforming growth factor alpha (TGFalpha), hepatocyte growth factor (HGF) and acidic fibroblast growth factor (aFGF) are the three major growth fa...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.5.931
更新日期:1996-05-01 00:00:00
abstract::Normal human fibroblasts do not synthesize platelet-derived growth factor (PDGF), but they respond to its mitogenic action. In contrast, cells from several human fibrosarcomas have been shown to synthesize PDGF at significant levels. To investigate the possible role of PDGF expression in the development of tumors of m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.10.2167
更新日期:1994-10-01 00:00:00
abstract::Aminoacyl-transfer ribonucleic acid (tRNA) synthetases-interacting multifunctional protein (AIMP) 2 is a factor associated with the macromolecular protein synthesis machinery consisting of nine different aminoacyl-tRNA synthetases and three non-enzymatic factors. However, it was shown to work as a multifaceted regulat...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp170
更新日期:2009-09-01 00:00:00
abstract::The purpose of this study was to examine the activity and associated kinetic parameters of epidermal protein kinase C (PKC) following stimulation by sn-1,2-dioctanoylglycerol (DIC8) or 12-O-tetradecanoylphorbol-13-acetate (TPA) and to examine the relationship between levels of epidermal PKC activity and the induction ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.5.833
更新日期:1989-05-01 00:00:00
abstract::Carcinogenicity of catechol, a naturally occurring and industrial chemical which has been shown to have strong cell proliferating potential on rat glandular stomach epithelium, was investigated in male and female F344 rats and B6C3F1 mice. Groups of 30 male and female F344 rats and B6C3F1 mice were treated with 0.8% c...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.3.525
更新日期:1993-03-01 00:00:00
abstract::This study evaluated the influence of genetic polymorphisms in the microsomal epoxide hydrolase (mEPHX) gene on lung cancer risk in a case-control study of two different ethnic groups, Mexican-Americans and African-Americans. There were 138 lung cancer cases (60 Mexican-American and 78 African-American) and 148 contro...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.6.923
更新日期:2001-06-01 00:00:00
abstract::Zuotin-related factor 1 (ZRF1) is a recently characterized epigenetic factor involved in transcriptional regulation and is highly overexpressed in several malignancies, but it is not known whether it plays a role in gastric cancer (GC). In this study, we investigated whether ZRF1 acts as a cancer-promoting gene throug...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx139
更新日期:2018-02-09 00:00:00