Abstract:
:Telomerase activity is observed in approximately 90% of human cancer including esophageal squamous cell cancer. Normal somatic cells do not display telomerase activity on a regular basis. The major mechanism to regulate telomerase activity in human cells is the transcriptional control of the catalytic subunit, the human reverse transcriptase gene hTERT. However, the manner in which telomerase activity is regulated during malignant transformation and whether this regulation is influenced by single genetic alterations important in this process are not well understood. In this study we investigated the transcriptional regulation and activity of human telomerase in a cellular model representing important known genetic alterations observed in esophageal cancer. We characterized the respective cells with regard to their telomere biology and telomerase expression, transcriptional regulation using promoter--as well as electrophoretic mobility shift assay--analyses and their promoter methylation status. We could demonstrate that telomerase expression and subsequent activity are differentially regulated in the progression from normal esophageal epithelial cells to genetically defined esophageal cells harboring a specific genetic alteration frequently found in esophageal cancer and compared those changes with esophageal cancer cells. Whereas primary esophageal cells are mainly regulated by Sp1, in cells harboring a genetic alteration as cyclin D1 overexpression other transcription factors like E2F and c-myc as well as promoter methylation influence hTERT transcription. This model demonstrates that the transcriptional regulation of telomerase is influenced by a given genetic alteration important in esophageal cancer, and therefore provides new insight in telomerase regulation during carcinogenesis.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Quante M,Heeg S,von Werder A,Goessel G,Fulda C,Doebele M,Nakagawa H,Beijersbergen R,Blum HE,Opitz OGdoi
10.1093/carcin/bgi153keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
1879-89issue
11eissn
0143-3334issn
1460-2180pii
bgi153journal_volume
26pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Oreochromis mossambicus, commonly known as tilapia, is a freshwater teleost with a wide tolerance to environmental conditions. Multiple focal lesions in the liver were observed 2 months after cessation of a one-month long treatment with 100 p.p.m. diethylnitrosamine. Cells were small and compact and arranged in sheets...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.3.493
更新日期:1989-03-01 00:00:00
abstract::In order to study the mechanism of cancer production by aflatoxin B1 (AFB1) in extrahepatic tissues which have relatively low cytochrome P450 monooxygenase (P450) activity, we have examined prostaglandin H synthase (PHS)-mediated AFB1 activation [( 3H]AFB1-DNA binding). [3H]AFB1 was activated by both purified PHS and ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.11.1915
更新日期:1990-11-01 00:00:00
abstract::BALB/c 3T3 A31-1-13 cells are highly susceptible to chemical-induced cell transformation and their gap-junctional intercellular communication (GJIC) is decreased when these cells become confluent. On the other hand, A31-1-1 cells do not show such a change in GJIC and are more resistant to chemical induction of transfo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.10.1923
更新日期:1991-10-01 00:00:00
abstract::Cyclin E is reported to be an important cell cycle regulator, and its dysregulation is implicated in tumorigenesis including esophageal squamous cell carcinoma (ESCC). MicroRNAs (miRNAs) regulate gene expression at the posttranscriptional level and play important roles in tumor initiation and progression. However, the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr078
更新日期:2011-07-01 00:00:00
abstract::It has previously been proposed that redox cycling between catechol estrogens and their quinones, mediated by cytochrome P450, could lead to the generation of free radicals that would subsequently cause oxidative damage to DNA and proteins that might have a role in hormonal carcinogenesis. Alternative, non-enzymatic m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.7.1421
更新日期:1994-07-01 00:00:00
abstract::The ability to repair damaged DNA was determined in different cell populations of rabbit lung cells isolated by centrifugal elutriation. DNA excision repair, measured as unscheduled DNA synthesis, was examined in in vitro confluent primary cultures. A dose dependent level of DNA excision repair was observed in alveola...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.4.661
更新日期:1985-04-01 00:00:00
abstract::We describe studies to determine if susceptibility to pituitary tumours is associated with the putatively high risk GSTM1 null and CYP2D6 EM genotypes. Frequency distributions of these genotypes were similar in cases and controls though the frequency of CYP2D6 PM and GSTM1 B tended to be lower (P = 0.072 and P = 0.095...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.7.1643
更新日期:1995-07-01 00:00:00
abstract::Reactive oxygen species (ROS) have been implicated in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) resistance of many cancers. We evaluated the role of peroxiredoxin (Prx) I in TRAIL resistance governed by coupling of nicotinamide adenosine dinucleotide phosphate oxidase (Nox)-derived ROS signaling ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp104
更新日期:2009-07-01 00:00:00
abstract::3-Amino-1,2,4-triazole, a thyroid carcinogen and goitrogen, is negative in a wide variety of short-term mutagenicity assays. However, amitrole induces gene mutations and morphological transformation in Syrian hamster embryo fibroblasts, cells known to carry out the prostaglandin H synthase (PHS)-mediated peroxidative ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.5.659
更新日期:1987-05-01 00:00:00
abstract::Herbacetin is a flavonol compound that is found in plants such as flaxseed and ramose scouring rush herb, it possesses a strong antioxidant capacity, and exerts anticancer effects on colon and breast cancer. However, the effect of herbacetin on skin cancer has not been investigated. Herein, we identified herbacetin as...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx082
更新日期:2017-10-26 00:00:00
abstract::We previously demonstrated that a single dose of gamma-radiation (350 rads) was able to induce thymic lymphomas in C57BL mice when followed by promoting treatment with oral cyclosporine (CsA), a non-genotoxic immunosuppressant. We have now tested the efficacy of various doses of gamma-radiation as an initiator of CsA ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.1.43
更新日期:1991-01-01 00:00:00
abstract::Extracts of human urine were shown to contain substances that strongly inhibited the liver S9-mediated mutagenicity of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in Salmonella typhimurium TA98 strain in a liquid incubation assay. The inhibitory effect was unrelated to cytotoxicity and was similar with urin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.12.2317
更新日期:1992-12-01 00:00:00
abstract::The effects of exposure to benzo[a]pyrene (BAP) and formaldehyde (HCHO), alone and combined, on the induction of carcinogenesis in rat tracheal implants was determined as the number of growth-altered cell populations (tumor-initiation sites, TIS) per trachea. While exposure twice-weekly for 4.5 months to 0.2% HCHO sol...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.12.1951
更新日期:1987-12-01 00:00:00
abstract::Lung cancer is the leading cause of cancer-related mortality, with most cases attributed to tobacco smoking, in which nicotine-derived nitrosamine ketone (NNK) is the most potent lung carcinogen. The ADAM17 protease is responsible for the ectodomain shedding of many pro-tumorigenic cytokines, growth factors and recept...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgz123
更新日期:2020-06-17 00:00:00
abstract::Mutant RAS (RAS(V12)) is known to transform most immortal cells but to induce premature senescence in primary cells. RAS(V12)-induced senescence in murine cells depends on the induction of the ARF/p53 and the retinoblastoma (Rb) family tumor suppressor pathways. We and others have shown previously that oncogene-induce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp296
更新日期:2010-02-01 00:00:00
abstract::Overexpressed DNA methyltransferase 1 (DNMT1) strongly contributes to tumor suppressor gene silencing in colorectal cancer (CRC). However, the underlying mechanism of DNMT1 overexpression is still unclear. MicroRNAs (miRNA) have been implicated as gene regulators controlling diverse biological processes, including car...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr081
更新日期:2011-07-01 00:00:00
abstract::The Notch pathway plays a key role in the development and is increasingly recognized for its importance in cancer. We demonstrated previously the overexpression of Notch-1 and its ligands in gliomas and showed that their knockdown inhibits glioma cell proliferation and survival. To elucidate the mechanisms downstream ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn079
更新日期:2008-05-01 00:00:00
abstract::A two-stage carcinogenesis experiment was performed in Syrian golden hamsters using a single intragastric initiation with 10 mg/kg body weight of 7,12-dimethylbenz[a]anthracene (DMBA) and repetitive topical promotion on the back skin with two different doses (80 and 160 mg, respectively) of benzoyl peroxide in 1 ml ac...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.3.479
更新日期:1987-03-01 00:00:00
abstract::This report shows that generation of hydrogen peroxide (H2O2) by human polymorphonuclear leukocytes (PMNs) activated with tumor promoters of varying potency as first and second stage promoters correlates well with activities of these promoters in vivo. Those tested were 12-O-tetradecanoylphorbol-13-acetate (TPA), a co...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.3.455
更新日期:1987-03-01 00:00:00
abstract::Environmental contributions to cancer development are widely accepted, but only a fraction of all pertinent exposures have probably been identified. Traditional toxicological approaches to the problem have largely focused on the effects of individual agents at singular endpoints. As such, they have incompletely addres...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgv037
更新日期:2015-06-01 00:00:00
abstract::Peroxisome proliferator-activated receptor (PPAR) gamma is a ligand-activated nuclear receptor that plays a key role in adipogenesis and adipocyte gene expression, and has recently been linked with possible antineoplastic effects in colonic carcinogenesis. PPARgamma2 and gamma3 are two transcripts arising from the PPA...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl001
更新日期:2006-09-01 00:00:00
abstract::Previous studies have shown that TIPE1 inhibits tumor proliferation and metastasis in certain cancers; however, increased expression of TIPE1 is observed in cervical cancer cell lines and tissues, indicating it might exert a distinctive role in cervical cancer. Cell and xenograft tumorigenicity assays showed that TIPE...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy163
更新日期:2019-06-10 00:00:00
abstract::Amino acids (AAs) are biologically important nutrient compounds necessary for the survival of any cell. Of the 20 AAs, cancer cells depend on the uptake of several extracellular AAs for survival. However, which extracellular AA is indispensable for the survival of cancer cells and the molecular mechanism involved have...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy033
更新日期:2018-05-28 00:00:00
abstract::The androgen receptor (AR) is involved in the development and progression of prostate cancer. In order to find new compounds that may present novel mechanisms to attenuate the function of AR, we investigated the effect of a natural flavonoid chemical, quercetin, on androgen action in an androgen-responsive LNCaP prost...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.3.409
更新日期:2001-03-01 00:00:00
abstract::X-ray repair cross-complementing group 1 (XRCC1) and xeroderma pigmentosum group D (XPD) are mainly involved in base excision repair (BER) and nucleotide excision repair (NER) of DNA repair pathways, respectively. Polymorphisms of DNA repair gene XRCC1 and XPD has recently been identified, and there is a growing body ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.8.1321
更新日期:2002-08-01 00:00:00
abstract::It is now unanimously accepted that neoplastic cells tend to become less susceptible to the growth regulatory effects of transforming growth factor-beta1 (TGF-beta1), mainly because of reduced expression and/or activity of TGF-beta1-specific receptors, as reported for many human cancers including colon cancer. Consequ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn106
更新日期:2008-05-01 00:00:00
abstract::Cancer-associated fibroblasts (CAFs) have been described to play critical roles in initiation, progression and metastasis of various cancers. However, the involvement of CAFs in oral cancer (OC) has not been well addressed. In this study, we demonstrate that CAFs, when cocultured with OC cells (OCCs), produce high lev...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgu046
更新日期:2014-06-01 00:00:00
abstract::An antimutagenic compound was isolated from the leaves of Carmona retusa (Vahl) Masam. Its structure has been elucidated by spectral analysis to be 4-hydroxy-7,8,11,12,15,7',8',11',12',15'-decahydro-beta, psi-carotene. The results of the Micronucleus Test, an in vivo method, showed that the isolated antimutagenic comp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.1.123
更新日期:1993-01-01 00:00:00
abstract::Liver DNA, obtained at various time intervals from rats chronically fed a choline-devoid diet, was analysed for the presence of aromatic or alkyl adducts by the 32P-postlabeling assay. Alkyl adducts were not detected. Aromatic DNA adduct lesions were revealed, but only at levels (1 adduct per 0.5-3 X 10(9) nucleotides...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.1.187
更新日期:1987-01-01 00:00:00
abstract::Mutations in codon 61 of the Ha-ras proto-oncogene have been shown to occur with a high frequency in both spontaneous and carcinogen-induced liver tumours of the B6C3F1 mouse. In the present study we analysed the frequency and pattern of Ha-ras mutations in liver tumours in a different strain of mice, namely the CF1 m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.10.1875
更新日期:1990-10-01 00:00:00