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Omics profiles used to evaluate the gene expression of Exiguobacterium antarcticum B7 during cold adaptation.

Abstract：

BACKGROUND:Exiguobacterium antarcticum strain B7 is a Gram-positive psychrotrophic bacterial species isolated in Antarctica. Although this bacteria has been poorly studied, its genome has already been sequenced. Therefore, it is an appropriate model for the study of thermal adaptation. In the present study, we analyzed the transcriptomes and proteomes of E. antarcticum B7 grown at 0°C and 37°C by SOLiD RNA-Seq, Ion Torrent RNA-Seq and two-dimensional difference gel electrophoresis tandem mass spectrometry (2D-DIGE-MS/MS). RESULTS:We found expression of 2,058 transcripts in all replicates from both platforms and differential expression of 564 genes (absolute log2FC≥1, P-value<0.001) comparing the two temperatures by RNA-Seq. A total of 73 spots were differentially expressed between the two temperatures on 2D-DIGE, 25 of which were identified by MS/MS. Some proteins exhibited patterns of dispersion in the gel that are characteristic of post-translational modifications. CONCLUSIONS:Our findings suggest that the two sequencing platforms yielded similar results and that different omic approaches may be used to improve the understanding of gene expression. To adapt to low temperatures, E. antarcticum B7 expresses four of the six cold-shock proteins present in its genome. The cold-shock proteins were the most abundant in the bacterial proteome at 0°C. Some of the differentially expressed genes are required to preserve transcription and translation, while others encode proteins that contribute to the maintenance of the intracellular environment and appropriate protein folding. The results denote the complexity intrinsic to the adaptation of psychrotrophic organisms to cold environments and are based on two omic approaches. They also unveil the lifestyle of a bacterial species isolated in Antarctica.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Dall'Agnol HP,Baraúna RA,de Sá PH,Ramos RT,Nóbrega F,Nunes CI,das Graças DA,Carneiro AR,Santos DM,Pimenta AM,Carepo MS,Azevedo V,Pellizari VH,Schneider MP,Silva A

doi

10.1186/1471-2164-15-986

subject

Has Abstract

pub_date

2014-11-18 00:00:00

pages

986

issn

1471-2164

pii

1471-2164-15-986

journal_volume

pub_type

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Omics profiles used to evaluate the gene expression of Exiguobacterium antarcticum B7 during cold adaptation.