Abstract:
BACKGROUND:Bloodstream malaria parasites require Ca++ for their development, but the sites and mechanisms of Ca++ utilization are not well understood. We hypothesized that there may be differences in Ca++ uptake or utilization by genetically distinct lines of P. falciparum. These differences, if identified, may provide insights into molecular mechanisms. RESULTS:Dose response studies with the Ca++ chelator EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid) revealed stable differences in Ca++ requirement for six geographically divergent parasite lines used in previous genetic crosses, with the largest difference seen between the parents of the HB3 x Dd2 cross. Genetic mapping of Ca++ requirement yielded complex inheritance in 34 progeny clones with a single significant locus on chromosome 7 and possible contributions from other loci. Although encoded by a gene in the significant locus and a proposed Ca++ target, PfCRT (P. falciparum chloroquine resistance transporter), the primary determinant of clinical resistance to the antimalarial drug chloroquine, does not appear to contribute to this quantitative trait. Stage-specific application of extracellular EGTA also excluded determinants associated with merozoite egress and erythrocyte reinvasion. CONCLUSIONS:We have identified differences in Ca++ utilization amongst P. falciparum lines. These differences are under genetic regulation, segregating as a complex trait in genetic cross progeny. Ca++ uptake and utilization throughout the bloodstream asexual cycle of malaria parasites represents an unexplored target for therapeutic intervention.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Apolis L,Olivas J,Srinivasan P,Kushwaha AK,Desai SAdoi
10.1186/s12864-018-5418-ysubject
Has Abstractpub_date
2019-01-16 00:00:00pages
47issue
1issn
1471-2164pii
10.1186/s12864-018-5418-yjournal_volume
20pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:Natural contamination and anthropogenic pollution of soils are likely to be major determinants of functioning and survival of keystone invertebrate taxa. Soil animals will have both evolutionary adaptation and genetically programmed responses to these toxic chemicals, but mechanistic understanding of such is...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-266
更新日期:2008-06-03 00:00:00
abstract:BACKGROUND:The emergence of vertebrates is characterized by a strong increase in miRNA families. MicroRNAs interact broadly with many transcripts, and the evolution of such a system is intriguing. However, evolutionary questions concerning the origin of miRNA genes and their subsequent evolution remain unexplained. RE...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-718
更新日期:2012-12-22 00:00:00
abstract:BACKGROUND:Chromosome 17q21.31 contains a common inversion polymorphism of approximately 900 kb in populations with European ancestry. Two divergent MAPT haplotypes, H1 and H2 are described with distinct linkage disequilibrium patterns across the region reflecting the inversion status at this locus. The MAPT H1 haploty...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-458
更新日期:2012-09-06 00:00:00
abstract:BACKGROUND:Oxidative stress is a common stress encountered by living organisms and is due to an imbalance between intracellular reactive oxygen and nitrogen species (ROS, RNS) and cellular antioxidant defence. To defend themselves against ROS/RNS, bacteria possess a subsystem of detoxification enzymes, which are classi...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-637
更新日期:2008-12-31 00:00:00
abstract:BACKGROUND:Missing data is an inevitable phenomenon in gene expression microarray experiments due to instrument failure or human error. It has a negative impact on performance of downstream analysis. Technically, most existing approaches suffer from this prevalent problem. Imputation is one of the frequently used metho...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-16-S9-S1
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Starches are the main storage polysaccharides in plants and are distributed widely throughout plants including seeds, roots, tubers, leaves, stems and so on. Currently, microscopic observation is one of the most important ways to investigate and analyze the structure of starches. The position, shape, and siz...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-S2-S13
更新日期:2010-11-02 00:00:00
abstract:BACKGROUND:Transposable Elements (TEs) are key components that shape the organization and evolution of genomes. Fungi have developed defense mechanisms against TE invasion such as RIP (Repeat-Induced Point mutation), MIP (Methylation Induced Premeiotically) and Quelling (RNA interference). RIP inactivates repeated sequ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1347-1
更新日期:2015-02-28 00:00:00
abstract:BACKGROUND:Increasing evidence has shown that circular RNAs (circRNAs) are involved in neurodegenerative disorders, but their roles in neurological toxoplasmosis are yet to know. This study examined miRNA and circRNA expressions in mouse brain following oral infection with T. gondii Pru strain. RESULTS:Total RNA extra...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-6464-9
更新日期:2020-01-14 00:00:00
abstract:BACKGROUND:The SOS response is a well-known regulatory network present in most bacteria and aimed at addressing DNA damage. It has also been linked extensively to stress-induced mutagenesis, virulence and the emergence and dissemination of antibiotic resistance determinants. Recently, the SOS response has been shown to...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-58
更新日期:2012-02-03 00:00:00
abstract:BACKGROUND:Interactions among transcription factors (TFs) and histone modifications (HMs) play an important role in the precise regulation of gene expression. The context specificity of those interactions and further its dynamics in normal and disease remains largely unknown. Recent development in genomics technology e...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5278-5
更新日期:2018-12-31 00:00:00
abstract:BACKGROUND:Crustaceans have been studied extensively as model systems for nervous system function from single neuron properties to behavior. However, lack of molecular sequence information and tools have slowed the adoption of these physiological systems as molecular model systems. In this study, we sequenced and perfo...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3215-z
更新日期:2016-11-04 00:00:00
abstract:BACKGROUND:Development of crop varieties with high nitrogen use efficiency (NUE) is crucial for minimizing N loss, reducing environmental pollution and decreasing input cost. Maize is one of the most important crops cultivated worldwide and its productivity is closely linked to the amount of fertilizer used. A survey o...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-77
更新日期:2014-01-28 00:00:00
abstract:BACKGROUND:Bacteriophages are the most abundant and diverse entities in the biosphere, and this diversity is driven by constant predator-prey evolutionary dynamics and horizontal gene transfer. Phage genome sequences are under-sampled and therefore present an untapped and uncharacterized source of genetic diversity, ty...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5647-8
更新日期:2019-05-09 00:00:00
abstract:BACKGROUND:The tuberous root of sweet potato is an important agricultural and biological organ. There are not sufficient transcriptomic and genomic data in public databases for understanding of the molecular mechanism underlying the tuberous root formation and development. Thus, high throughput transcriptome sequencing...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-726
更新日期:2010-12-24 00:00:00
abstract:BACKGROUND:Simple sequence repeats (SSRs) have become widely used as molecular markers in plant genetic studies due to their abundance, high allelic variation at each locus and simplicity to analyze using conventional PCR amplification. To study plants with unknown genome sequence, SSR markers from Expressed Sequence T...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3328-4
更新日期:2016-12-22 00:00:00
abstract:BACKGROUND:Tandem mass spectrometry (MS/MS) has become a standard method for identification of proteins extracted from biological samples but the huge number and the noise contamination of MS/MS spectra obstruct swift and reliable computer-aided interpretation. Typically, a minor fraction of the spectra per sample (mos...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-S1-S13
更新日期:2010-02-10 00:00:00
abstract:BACKGROUND:Association mapping approaches are dependent upon discovery and validation of single nucleotide polymorphisms (SNPs). To further association studies in Anopheles gambiae we conducted a major resequencing programme, primarily targeting regions within or close to candidate genes for insecticide resistance. RE...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-320
更新日期:2009-07-16 00:00:00
abstract:BACKGROUND:Sterol esterases and lipases are enzymes able to efficiently catalyze synthesis and hydrolysis reactions of both sterol esters and triglycerides and due to their versatility could be widely used in different industrial applications. Lipases with this ability have been reported in the yeast Candida rugosa tha...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-712
更新日期:2013-10-18 00:00:00
abstract:BACKGROUND:Rice false smut caused by Villosiclava virens is a devastating fungal disease that spreads in major rice-growing regions throughout the world. However, the genomic information for this fungal pathogen is limited and the pathogenic mechanism of this disease is still not clear. To facilitate genetic, molecular...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-883
更新日期:2013-12-16 00:00:00
abstract:BACKGROUND:Chinese wild grapevine (Vitis amurensis) has remarkable cold stress tolerance, exceeding that of the common cultivated grapevine (Vitis vinifera L.). RESULT:Here, we surveyed the expression dynamics of microRNAs (miRNAs) across Chinese wild grapevine (cv. Beibinghong) and cultivated grapevine (cv. Cabernet ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6111-5
更新日期:2019-10-15 00:00:00
abstract:BACKGROUND:Nicotiana attenuata (coyote tobacco) is an ecological model for studying plant-environment interactions and plant gene function under real-world conditions. During the last decade, large amounts of genomic, transcriptomic and metabolomic data have been generated with this plant which has provided new insight...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3465-9
更新日期:2017-01-13 00:00:00
abstract:BACKGROUND:Progress in the fields of protein separation and identification technologies has accelerated research into biofluids proteomics for protein biomarker discovery. Urine has become an ideal and rich source of biomarkers in clinical proteomics. Here we performed a proteomic analysis of urine samples from pregnan...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-777
更新日期:2013-11-11 00:00:00
abstract:BACKGROUND:Whole exome sequencing studies identify hundreds to thousands of rare protein coding variants of ambiguous significance for human health. Computational tools are needed to accelerate the identification of specific variants and genes that contribute to human disease. RESULTS:We have developed the Variant Eff...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-S3-S3
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Juncus effusus L. (family: Juncaceae; order: Poales) is a helophytic rush growing in temperate damp or wet terrestrial habitats and is of almost cosmopolitan distribution. The species has been studied intensively with respect to its interaction with co-occurring plants as well as microbes being involved in m...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5886-8
更新日期:2019-06-13 00:00:00
abstract:BACKGROUND:The sRNAs of bacterial pathogens are known to be involved in various cellular roles including environmental adaptation as well as regulation of virulence and pathogenicity. It is expected that sRNAs may also have similar functions for Burkholderia pseudomallei, a soil bacterium that can adapt to diverse envi...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S7-S13
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Wild sugarcane Saccharum spontaneum plants vary in ploidy, which complicates the utilization of its germplasm in sugarcane breeding. Investigations on cold tolerance in relation to different ploidies in S. spontaneum may promote the exploitation of its germplasm and accelerate the improvement of sugarcane va...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06917-z
更新日期:2020-07-22 00:00:00
abstract:BACKGROUND:Clostridium sticklandii belongs to a cluster of non-pathogenic proteolytic clostridia which utilize amino acids as carbon and energy sources. Isolated by T.C. Stadtman in 1954, it has been generally regarded as a "gold mine" for novel biochemical reactions and is used as a model organism for studying metabol...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-555
更新日期:2010-10-11 00:00:00
abstract:BACKGROUND:LEF1/TCF transcription factors and their activator β-catenin are effectors of the canonical Wnt pathway. Although Wnt/β-catenin signaling has been implicated in neurodegenerative and psychiatric disorders, its possible role in the adult brain remains enigmatic. To address this issue, we sought to identify th...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-635
更新日期:2012-11-17 00:00:00
abstract:BACKGROUND:The rapid growth of the world's population demands an increase in food production that no longer can be reached by increasing amounts of nitrogenous fertilizers. Plant growth promoting bacteria (PGPB) might be an alternative to increase nitrogenous use efficiency (NUE) in important crops such wheat. Azospiri...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-378
更新日期:2014-05-16 00:00:00
abstract:BACKGROUND:Although hatching is perhaps the most abrupt and profound metabolic challenge that a chicken must undergo; there have been no attempts to functionally map the metabolic pathways induced in liver during the embryo-to-hatchling transition. Furthermore, we know very little about the metabolic and regulatory fac...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5080-4
更新日期:2018-09-21 00:00:00