Cronobacter, the emergent bacterial pathogen Enterobacter sakazakii comes of age; MLST and whole genome sequence analysis.

Abstract:

BACKGROUND:Following the association of Cronobacter spp. to several publicized fatal outbreaks in neonatal intensive care units of meningitis and necrotising enterocolitis, the World Health Organization (WHO) in 2004 requested the establishment of a molecular typing scheme to enable the international control of the organism. This paper presents the application of Next Generation Sequencing (NGS) to Cronobacter which has led to the establishment of the Cronobacter PubMLST genome and sequence definition database (http://pubmlst.org/cronobacter/) containing over 1000 isolates with metadata along with the recognition of specific clonal lineages linked to neonatal meningitis and adult infections RESULTS:Whole genome sequencing and multilocus sequence typing (MLST) has supports the formal recognition of the genus Cronobacter composed of seven species to replace the former single species Enterobacter sakazakii. Applying the 7-loci MLST scheme to 1007 strains revealed 298 definable sequence types, yet only C. sakazakii clonal complex 4 (CC4) was principally associated with neonatal meningitis. This clonal lineage has been confirmed using ribosomal-MLST (51-loci) and whole genome-MLST (1865 loci) to analyse 107 whole genomes via the Cronobacter PubMLST database. This database has enabled the retrospective analysis of historic cases and outbreaks following re-identification of those strains. CONCLUSIONS:The Cronobacter PubMLST database offers a central, open access, reliable sequence-based repository for researchers. It has the capacity to create new analysis schemes 'on the fly', and to integrate metadata (source, geographic distribution, clinical presentation). It is also expandable and adaptable to changes in taxonomy, and able to support the development of reliable detection methods of use to industry and regulatory authorities. Therefore it meets the WHO (2004) request for the establishment of a typing scheme for this emergent bacterial pathogen. Whole genome sequencing has additionally shown a range of potential virulence and environmental fitness traits which may account for the association of C. sakazakii CC4 pathogenicity, and propensity for neonatal CNS.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Forsythe SJ,Dickins B,Jolley KA

doi

10.1186/1471-2164-15-1121

subject

Has Abstract

pub_date

2014-12-16 00:00:00

pages

1121

issn

1471-2164

pii

1471-2164-15-1121

journal_volume

15

pub_type

杂志文章
  • Construction of high-resolution genetic maps of Zoysia matrella (L.) Merrill and applications to comparative genomic analysis and QTL mapping of resistance to fall armyworm.

    abstract:BACKGROUND:Zoysia matrella, widely used in lawns and sports fields, is of great economic and ecological value. Z. matrella is an allotetraploid species (2n = 4x = 40) in the genus zoysia under the subfamily Chloridoideae. Despite its ecological impacts and economic importance, the subfamily Chloridoideae has received l...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2969-7

    authors: Huang X,Wang F,Singh R,Reinert JA,Engelke MC,Genovesi AD,Chandra A,Yu Q

    更新日期:2016-08-08 00:00:00

  • Identification and transcriptomic profiling of genes involved in increasing sugar content during salt stress in sweet sorghum leaves.

    abstract:BACKGROUND:Sweet sorghum is an annual C4 crop considered to be one of the most promising bio-energy crops due to its high sugar content in stem, yet it is poorly understood how this plant increases its sugar content in response to salt stress. In response to high NaCl, many of its major processes, such as photosynthesi...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1760-5

    authors: Sui N,Yang Z,Liu M,Wang B

    更新日期:2015-07-19 00:00:00

  • CLARK: fast and accurate classification of metagenomic and genomic sequences using discriminative k-mers.

    abstract:BACKGROUND:The problem of supervised DNA sequence classification arises in several fields of computational molecular biology. Although this problem has been extensively studied, it is still computationally challenging due to size of the datasets that modern sequencing technologies can produce. RESULTS:We introduce CLA...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1419-2

    authors: Ounit R,Wanamaker S,Close TJ,Lonardi S

    更新日期:2015-03-25 00:00:00

  • Genes associated with the cis-regulatory functions of intragenic LINE-1 elements.

    abstract:BACKGROUND:Thousands of intragenic long interspersed element 1 sequences (LINE-1 elements or L1s) reside within genes. These intragenic L1 sequences are conserved and regulate the expression of their host genes. When L1 methylation is decreased, either through chemical induction or in cancer, the intragenic L1 transcri...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-14-205

    authors: Wanichnopparat W,Suwanwongse K,Pin-On P,Aporntewan C,Mutirangura A

    更新日期:2013-03-27 00:00:00

  • The gonadal transcriptome of the unisexual Amazon molly Poecilia formosa in comparison to its sexual ancestors, Poecilia mexicana and Poecilia latipinna.

    abstract:BACKGROUND:The unisexual Amazon molly (Poecilia formosa) originated from a hybridization between two sexual species, the sailfin molly (Poecilia latipinna) and the Atlantic molly (Poecilia mexicana). The Amazon molly reproduces clonally via sperm-dependent parthenogenesis (gynogenesis), in which the sperm of closely re...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-4382-2

    authors: Schedina IM,Groth D,Schlupp I,Tiedemann R

    更新日期:2018-01-03 00:00:00

  • Transcriptomic profiling of Burkholderia phymatum STM815, Cupriavidus taiwanensis LMG19424 and Rhizobium mesoamericanum STM3625 in response to Mimosa pudica root exudates illuminates the molecular basis of their nodulation competitiveness and symbiotic ev

    abstract:BACKGROUND:Rhizobial symbionts belong to the classes Alphaproteobacteria and Betaproteobacteria (called "alpha" and "beta"-rhizobia). Most knowledge on the genetic basis of symbiosis is based on model strains belonging to alpha-rhizobia. Mimosa pudica is a legume that offers an excellent opportunity to study the adapta...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4487-2

    authors: Klonowska A,Melkonian R,Miché L,Tisseyre P,Moulin L

    更新日期:2018-01-30 00:00:00

  • Unique aspects of fiber degradation by the ruminal ethanologen Ruminococcus albus 7 revealed by physiological and transcriptomic analysis.

    abstract:BACKGROUND:Bacteria in the genus Ruminococcus are ubiquitous members of the mammalian gastrointestinal tract. In particular, they are important in ruminants where they digest a wide range of plant cell wall polysaccharides. For example, Ruminococcus albus 7 is a primary cellulose degrader that produces acetate usable b...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-1066

    authors: Christopherson MR,Dawson JA,Stevenson DM,Cunningham AC,Bramhacharya S,Weimer PJ,Kendziorski C,Suen G

    更新日期:2014-12-04 00:00:00

  • Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting.

    abstract:BACKGROUND:Domestic broiler chickens rapidly accumulate adipose tissue due to intensive genetic selection for rapid growth and are naturally hyperglycemic and insulin resistant, making them an attractive addition to the suite of rodent models used for studies of obesity and type 2 diabetes in humans. Furthermore, chick...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-441

    authors: Ji B,Ernest B,Gooding JR,Das S,Saxton AM,Simon J,Dupont J,Métayer-Coustard S,Campagna SR,Voy BH

    更新日期:2012-08-31 00:00:00

  • TSCC: Two-Stage Combinatorial Clustering for virtual screening using protein-ligand interactions and physicochemical features.

    abstract:BACKGROUND:The increasing numbers of 3D compounds and protein complexes stored in databases contribute greatly to current advances in biotechnology, being employed in several pharmaceutical and industrial applications. However, screening and retrieving appropriate candidates as well as handling false positives presents...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-S4-S26

    authors: Clinciu DL,Chen YF,Ko CN,Lo CC,Yang JM

    更新日期:2010-12-02 00:00:00

  • Genome-wide analysis of consistently RNA edited sites in human blood reveals interactions with mRNA processing genes and suggests correlations with cell types and biological variables.

    abstract:BACKGROUND:A-to-I RNA editing is a co-/post-transcriptional modification catalyzed by ADAR enzymes, that deaminates Adenosines (A) into Inosines (I). Most of known editing events are located within inverted ALU repeats, but they also occur in coding sequences and may alter the function of encoded proteins. RNA editing ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5364-8

    authors: Giacopuzzi E,Gennarelli M,Sacco C,Filippini A,Mingardi J,Magri C,Barbon A

    更新日期:2018-12-27 00:00:00

  • Genomic analysis of nontypeable pneumococci causing invasive pneumococcal disease in South Africa, 2003-2013.

    abstract:BACKGROUND:The capsular polysaccharide is the principal virulence factor of Streptococcus pneumoniae and a target for current pneumococcal vaccines. However, some pathogenic pneumococci are serologically nontypeable [nontypeable pneumococci (NTPn)]. Due to their relative rarity, NTPn are poorly characterized, and, as s...

    journal_title:BMC genomics

    pub_type: 历史文章,杂志文章

    doi:10.1186/s12864-016-2808-x

    authors: Mohale T,Wolter N,Allam M,Ndlangisa K,Crowther-Gibson P,du Plessis M,von Gottberg A

    更新日期:2016-06-22 00:00:00

  • Genome-wide analysis of purple acid phosphatase structure and expression in ten vegetable species.

    abstract:BACKGROUND:Acquisition of external phosphorus (P) and optimisation of internal P are essential for plant growth and development, and insufficient availability of P in soils is a major challenge in agriculture. Members of the purple acid phosphatase (PAP) family of enzymes are candidates for increasing P use efficiency....

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5022-1

    authors: Xie L,Shang Q

    更新日期:2018-08-31 00:00:00

  • Population genomics and climate adaptation of a C4 perennial grass, Panicum hallii (Poaceae).

    abstract:BACKGROUND:Understanding how and why genetic variation is partitioned across geographic space is of fundamental importance to understanding the nature of biological species. How geographical isolation and local adaptation contribute to the formation of ecotypically differentiated groups of plants is just beginning to b...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5179-7

    authors: Gould BA,Palacio-Mejia JD,Jenkins J,Mamidi S,Barry K,Schmutz J,Juenger TE,Lowry DB

    更新日期:2018-11-01 00:00:00

  • Fast multiclonal clusterization of V(D)J recombinations from high-throughput sequencing.

    abstract:BACKGROUND:V(D)J recombinations in lymphocytes are essential for immunological diversity. They are also useful markers of pathologies. In leukemia, they are used to quantify the minimal residual disease during patient follow-up. However, the full breadth of lymphocyte diversity is not fully understood. RESULTS:We prop...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-409

    authors: Giraud M,Salson M,Duez M,Villenet C,Quief S,Caillault A,Grardel N,Roumier C,Preudhomme C,Figeac M

    更新日期:2014-05-28 00:00:00

  • Identification of functional regulatory elements in the human genome using pooled CRISPR screens.

    abstract:BACKGROUND:Genome-scale pooled CRISPR screens are powerful tools for identifying genetic dependencies across varied cellular processes. The vast majority of CRISPR screens reported to date have focused exclusively on the perturbation of protein-coding gene function. However, protein-coding genes comprise < 2% of the se...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-6497-0

    authors: Borys SM,Younger ST

    更新日期:2020-01-31 00:00:00

  • Anthocyanin biosynthetic genes in Brassica rapa.

    abstract:BACKGROUND:Anthocyanins are a group of flavonoid compounds. As a group of important secondary metabolites, they perform several key biological functions in plants. Anthocyanins also play beneficial health roles as potentially protective factors against cancer and heart disease. To elucidate the anthocyanin biosynthetic...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-426

    authors: Guo N,Cheng F,Wu J,Liu B,Zheng S,Liang J,Wang X

    更新日期:2014-06-04 00:00:00

  • Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions.

    abstract:BACKGROUND:In contrast to unidirectional promoters wherein antisense transcription results in short transcripts which are rapidly degraded, bidirectional promoters produce mature transcripts in both sense and antisense orientation. To understand the molecular mechanism of how productive bidirectional transcription is r...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4697-7

    authors: Jangid RK,Kelkar A,Muley VY,Galande S

    更新日期:2018-05-02 00:00:00

  • Altered gene expression in the superior temporal gyrus in schizophrenia.

    abstract:BACKGROUND:The superior temporal gyrus (STG), which encompasses the primary auditory cortex, is believed to be a major anatomical substrate for speech, language and communication. The STG connects to the limbic system (hippocampus and amygdala), the thalamus and neocortical association areas in the prefrontal cortex, a...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-9-199

    authors: Bowden NA,Scott RJ,Tooney PA

    更新日期:2008-04-29 00:00:00

  • Characterization of SR3 reveals abundance of non-LTR retrotransposons of the RTE clade in the genome of the human blood fluke, Schistosoma mansoni.

    abstract:BACKGROUND:It is becoming apparent that perhaps as much as half of the genome of the human blood fluke Schistosoma mansoni is constituted of mobile genetic element-related sequences. Non-long terminal repeat (LTR) retrotransposons, related to the LINE elements of mammals, comprise much of this repetitive component of t...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-6-154

    authors: Laha T,Kewgrai N,Loukas A,Brindley PJ

    更新日期:2005-11-04 00:00:00

  • Polyploidization and pseudogenization in allotetraploid frog Xenopus laevis promote the evolution of aquaporin family in higher vertebrates.

    abstract:BACKGROUND:Aquaporins (AQPs), as members of the major intrinsic protein (MIP) superfamily, facilitated the permeation of water and other solutes and are involved in multiple biological processes. AQP family exists in almost all living organisms and is highly diversified in vertebrates in both classification and functio...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-06942-y

    authors: Jia Y,Liu X

    更新日期:2020-07-29 00:00:00

  • Metabolic and evolutionary insights into the closely-related species Streptomyces coelicolor and Streptomyces lividans deduced from high-resolution comparative genomic hybridization.

    abstract:BACKGROUND:Whilst being closely related to the model actinomycete Streptomyces coelicolor A3(2), S. lividans 66 differs from it in several significant and phenotypically observable ways, including antibiotic production. Previous comparative gene hybridization studies investigating such differences have used low-density...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-682

    authors: Lewis RA,Laing E,Allenby N,Bucca G,Brenner V,Harrison M,Kierzek AM,Smith CP

    更新日期:2010-12-01 00:00:00

  • An updated explanation of ancestral karyotype changes and reconstruction of evolutionary trajectories to form Camelina sativa chromosomes.

    abstract:BACKGROUND:Belonging to lineage I of Brassicaceae, Camelina sativa is formed by two hybridizations of three species (three sub-genomes). The three sub-genomes were diverged from a common ancestor, likely derived from lineage I (Ancestral Crucifer karyotype, ACK). The karyotype evolutionary trajectories of the C. sativa...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-07081-0

    authors: Zhang Z,Meng F,Sun P,Yuan J,Gong K,Liu C,Wang W,Wang X

    更新日期:2020-10-12 00:00:00

  • Monophyly of clade III nematodes is not supported by phylogenetic analysis of complete mitochondrial genome sequences.

    abstract:BACKGROUND:The orders Ascaridida, Oxyurida, and Spirurida represent major components of zooparasitic nematode diversity, including many species of veterinary and medical importance. Phylum-wide nematode phylogenetic hypotheses have mainly been based on nuclear rDNA sequences, but more recently complete mitochondrial (m...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-392

    authors: Park JK,Sultana T,Lee SH,Kang S,Kim HK,Min GS,Eom KS,Nadler SA

    更新日期:2011-08-03 00:00:00

  • Chronic wounds alter the proteome profile in skin mucus of farmed gilthead seabream.

    abstract:BACKGROUND:Skin and its mucus are known to be the first barrier of defence against any external stressors. In fish, skin wounds frequently appear as a result of intensive culture and also some diseases have skin ulcers as external clinical signs. However, there is no information about the changes produced by the wounds...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-4349-3

    authors: Cordero H,Brinchmann MF,Cuesta A,Esteban MA

    更新日期:2017-12-02 00:00:00

  • A genome-wide analysis of the lysophosphatidate acyltransferase (LPAAT) gene family in cotton: organization, expression, sequence variation, and association with seed oil content and fiber quality.

    abstract:BACKGROUND:Lysophosphatidic acid acyltransferase (LPAAT) encoded by a multigene family is a rate-limiting enzyme in the Kennedy pathway in higher plants. Cotton is the most important natural fiber crop and one of the most important oilseed crops. However, little is known on genes coding for LPAATs involved in oil biosy...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-3594-9

    authors: Wang N,Ma J,Pei W,Wu M,Li H,Li X,Yu S,Zhang J,Yu J

    更新日期:2017-03-01 00:00:00

  • ArachnoServer: a database of protein toxins from spiders.

    abstract:BACKGROUND:Venomous animals incapacitate their prey using complex venoms that can contain hundreds of unique protein toxins. The realisation that many of these toxins may have pharmaceutical and insecticidal potential due to their remarkable potency and selectivity against target receptors has led to an explosion in th...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-375

    authors: Wood DL,Miljenović T,Cai S,Raven RJ,Kaas Q,Escoubas P,Herzig V,Wilson D,King GF

    更新日期:2009-08-13 00:00:00

  • Genomic diversity dynamics in conserved chicken populations are revealed by genome-wide SNPs.

    abstract:BACKGROUND:Maintaining maximum genetic diversity and preserving breed viability in conserved populations necessitates the rigorous evaluation of conservation schemes. Three chicken breeds (Baier Yellow Chicken (BEC), Beijing You Chicken (BYC) and Langshan Chicken (LSC)) are currently in conservation programs in China. ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4973-6

    authors: Zhang M,Han W,Tang H,Li G,Zhang M,Xu R,Liu Y,Yang T,Li W,Zou J,Wu K

    更新日期:2018-08-09 00:00:00

  • A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein.

    abstract:BACKGROUND:Effector proteins are translocated into host cells by plant-pathogens to undermine pattern-triggered immunity (PTI), the plant response to microbe-associated molecular patterns that interferes with the infection process. Individual effectors are found in variable repertoires where some constituents target th...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2534-4

    authors: Worley JN,Pombo MA,Zheng Y,Dunham DM,Myers CR,Fei Z,Martin GB

    更新日期:2016-03-14 00:00:00

  • Use of biological priors enhances understanding of genetic architecture and genomic prediction of complex traits within and between dairy cattle breeds.

    abstract:BACKGROUND:A better understanding of the genetic architecture underlying complex traits (e.g., the distribution of causal variants and their effects) may aid in the genomic prediction. Here, we hypothesized that the genomic variants of complex traits might be enriched in a subset of genomic regions defined by genes gro...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-4004-z

    authors: Fang L,Sahana G,Ma P,Su G,Yu Y,Zhang S,Lund MS,Sørensen P

    更新日期:2017-08-10 00:00:00

  • Altered patterns of gene duplication and differential gene gain and loss in fungal pathogens.

    abstract:BACKGROUND:Duplication, followed by fixation or random loss of novel genes, contributes to genome evolution. Particular outcomes of duplication events are possibly associated with pathogenic life histories in fungi. To date, differential gene gain and loss have not been studied at genomic scales in fungal pathogens, de...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-9-147

    authors: Powell AJ,Conant GC,Brown DE,Carbone I,Dean RA

    更新日期:2008-03-28 00:00:00