Abstract:
BACKGROUND:Therapeutic angiogenesis has been shown to promote blood vessel growth and improve tissue perfusion. Vascular endothelial growth factor (VEGF) plays an important role in angiogenesis. However, it has side effects that limit its therapeutic utility in vivo, especially at high concentrations. This study aimed to investigate whether an intramuscular injection of a genetically engineered zinc finger VEGF-activating transcription factor modulates the endothelial progenitor cells (EPC) and promotes therapeutic angiogenesis in a hindlimb ischemia model with type 1 diabetes. METHODS:Alloxan (intravenous injection) was used to induce type I diabetes in C57BL/6 mice (n = 58). The ischemic limb received ZFP-VEGF (125 µg ZFP-VEGF plasmid in 1% poloxamer) or placebo (1% poloxamer) intramuscularly. Mice were sacrificed 3, 5, 10, or 20 days post-injection. Limb blood flow was monitored using laser Doppler perfusion imaging. VEGF mRNA and protein expression were examined using real-time PCR and ELISA, respectively. Capillary density, proliferation, and apoptosis were examined using immunohistochemistry techniques. Flow cytometry was used to detect the EPC population in bone marrow. Two-tailed Student's paired t test and repeated-measures analysis of variance were used for statistical analysis. RESULTS:ZFP-VEGF increased VEGF mRNA and protein expression at 3 and 10 days post-injection, and increased EPC in bone marrow at day 5 and 20 post-injection compared with controls (P < 0.05). ZFP-VEGF treatment resulted in better perfusion recovery, a higher capillary density and proliferation, and less apoptosis compared with controls (P < 0.05). CONCLUSIONS:Intramuscular ZFP-VEGF injection promotes therapeutic angiogenesis in an ischemic hindlimb model with type 1 diabetes. This might be due to the effects of VEGF on cell survival and EPC recruitment.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Diao Y,Lian L,Guo L,Chen H,Chen Y,Song X,Li Ysubject
Has Abstractpub_date
2014-01-01 00:00:00pages
3623-9issue
20eissn
0366-6999issn
2542-5641journal_volume
127pub_type
杂志文章abstract::1 141 serum samples from various population groups in north China were examined for C100-3Ab by ELISA. Antibody to C100-3 antigen derived from HCV genome (C100-3A) and HBsAg were measured in 438 normal population in Beijing. The C100-3Ab positive rate was 2.1% and the HBsAg positive rate was 2.5%. There is increased o...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::The long-term results of total meniscectomy in 60 patients were reviewed. The patients were followed up to 10 to 33 years. 58.3% of the patients had excellent or good results. Statistical analysis showed that the late results were related to the time of follow-up and not associated with the duration of disease before ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract:BACKGROUND:Although the early clinical outcomes of total knee arthroplasty (TKA) using minimally invasive surgery techniques have been widely described, data on the mid- to long-term outcomes are limited. We designed a retrospective study to compare the two most common TKA techniques - The modified quadriceps-sparing (...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.160521
更新日期:2015-07-20 00:00:00
abstract:BACKGROUND:The effectiveness of neoadjuvant chemoradiotherapy (NCRT) treatment for patients with esophageal carcinoma (EC) remains controversial. The aim of this study was to compare the effect of NCRT followed by surgery (NCRTS) with surgery alone (SA) for EC. METHODS:The PubMed, EMBASE, and the Cochrane Library data...
journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析
doi:10.4103/0366-6999.195464
更新日期:2016-12-20 00:00:00
abstract:OBJECTIVE:This review focuses on the role of the large conductance calcium-activated potassium (BKCa) channels in diabetic vascular complications. DATA SOURCES:Relevant articles published in English or Chinese from 1981 to present were selected from PubMed. The search terms were "BKCa channels" and "diabetes". Importa...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Laparoscopic Heller cardiomyotomy and Dor fundoplication is the surgical procedure of choice for esophageal achalasia. The aim of this study was to investigate the clinical outcome of laparoscopic Heller-Dor procedure in our initial series of 25 patients with achalasia. METHODS:Between October 2003 and Janu...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-03-20 00:00:00
abstract:OBJECTIVE:To have a better understanding of genetic contributions to the development of obstructive sleep apnea hypopnea syndrome (OSAHS) by reviewing studies on its genetic basis. DATA SOURCES:A comprehensive search of the PubMed literature without restriction on the publication date was carried out using terms "obst...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Evidence suggests that various diseases may contribute to the circular RNAs (circRNAs) expression disorder. This review was aimed at looking for appropriate biomarkers for the treatment of diseases. DATA SOURCES:The comprehensive search used online literature databases including PubMed of National Center for...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.1097/CM9.0000000000000465
更新日期:2019-10-20 00:00:00
abstract::The vascular pedicled periosteum was used in 11 cases in our hospital for the treatment of femoral neck fracture with good result. The follow-up period ranged from 2 to 6 years, with an average of 4 years. Animal experiment was made to elucidate the repairing process. It demonstrated that the pedicled periosteum playe...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:Chronic obstructive pulmonary disease (COPD) is thought to be an inflammatory immune response disease. In most cases, the disease is caused by cigarette smoke, but it has been demonstrated that only 10% to 20% of smokers will definitely suffer from COPD. Dendritic cells (DCs) are considered to be the promote...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To compare the effect of ouabain on the blood pressure of rats with that of digoxin to find the evidences of the relationship between endogenous ouabain (EO) and development of hypertension. METHODS:Sprague-Dawley rats, which were divided into 3 groups, were infused with ouabain (23 x 75 micrograms.kg-1/day,...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND:Roundabout 5 (R5) is a monoclonal antibody which can neutralize the binding of Roundabout 1 (Robo1) to Slit2. Oral squamous cell carcinoma angiogenesis was significantly inhibited when R5 blocked slit-robo signaling pathway. However, the effect of R5 on the invasion of tongue cancer cells has not been invest...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-08-01 00:00:00
abstract::To develop a more comprehensive index for predicting the prognous of liver cirrhosis. 300 consecutive patients with cirrhosis were studied in terms of survival from 1975 to 1986. Median follow-up period was 5.3 years. A multivariable survival analysis (Cox's regression model) using clinical biochemical data obtained a...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract:BACKGROUND:Diffusion tensor imaging (DTI) permits quantitative examination within the pyramidal tract (PT) by measuring fractional anisotropy (FA). To the best of our knowledge, the inter-variability measures of FA along the PT remain unexplained. A clear understanding of these reference values would help radiologists ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-06-01 00:00:00
abstract::Free radicals in the gasphase of cigarette smoke were determined by spin trapping agent PBN (N-tert-butyl-2-phenyl nitrone) and DMPO (5,5-dimethyl-1-pyrroline-oxide) on electron spin resonance spectrometer. They were identified according to the spectrum parameters calculated from the spectra. The free radicals mainly ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::This review summarizes recent evidences regarding the potential influences of epidural labor analgesia (ELA) on the outcomes of neonates and children. Terms and relevant words including "ELA," "ELA and neonatal outcomes," "ELA and children's development," and "ELA and children's neurocognitive development" were used t...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000900
更新日期:2020-10-05 00:00:00
abstract:BACKGROUND:Among patients with advanced multivessel coronary disease, left ventricular (LV) function is widely variable, and clinical and angiographic correlates of ventricular dysfunction remain to be defined. METHODS:Among 73 339 patients undergoing diagnostic cardiac catheterization at a single center in China, pat...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Homoharringtonine (HHT) is a cephalotaxine ester derived from an evergreen tree found wildely throughout southern China, which has antileukemic activities against a variety of acute myeloid leukemic cells. For the sake of illustrating the mechanisms of HHT in the treatment of leukemia, we assessed the effect...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2005-03-20 00:00:00
abstract:BACKGROUND:Stereotactic radiosurgery is an alternative to resection of intracranial cerebral arteriovenous malformations (AVMs), while it will fail in some cases. This study aimed to evaluate the changes after stereotactic radiosurgery for AVMs. METHODS:Nineteen cases with cerebral AVMs had failure after stereotactic ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-06-20 00:00:00
abstract::Klinefelter’s syndrome (KS) is the most common sex chromosome disease in men. Classical features of the syndrome include a eunuchoidal body habitus, small testes and hypergonadotrophic hypogonadism. There has been an increased risk of diabetes mellitus and autoimmune disease for KS patients. This paper reports a case ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:Inner retinal oxygenation response (ΔPO(2)) is a worldwide study focus. However, the relevant reports on its radiological measurements are limited. In this study, magnetic resonance imaging (MRI), employing T1 weighted image (T1WI), was used to detect changes in ΔPO(2) following 100% oxygen inhalation in hum...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND:There are more than 300 genetic loci that have been found to be related to hereditary hearing impairment (HHI), including 92 causative genes for nonsyndromic hearing loss, among which 34 genes are related to autosomal dominant nonsyndromic HHI (ADNSHHI). Traditional linkage analysis and candidate gene sequen...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.171440
更新日期:2015-12-20 00:00:00
abstract:BACKGROUND:Rituximab is used extensively in combination with chemotherapy to cure non-Hodgkin's lymphoma (NHL), and not only accelerates short-term improvement, but also prolongs patient survival and decreases receptor relapse. The aim of this study was to evaluate the impact of Fcgamma IIIA (FcgammaRIIIA) gene polymor...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-01-20 00:00:00
abstract:BACKGROUND:Atrial fibrillation (AF) is the most common supraventricular arrhythmia in clinical practice. Chronic atrial fibrillation (CAF) is associated with ionic remodeling. However, little is known about the activity of ATP-sensitive potassium current (IK, ATP) during CAF. So we studied the changes of IK, ATP densit...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2005-07-20 00:00:00
abstract:BACKGROUND:Muscle fibers overlying the intramyocardial segment of an epicardial coronary artery are termed myocardial bridging (MB). Variable prevalence of MB has been described at autopsy and angiographic series with small and large sample size studies. In addition, no similar study was reported in Chinese population....
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-03-05 00:00:00
abstract:BACKGROUND:Coronary atherosclerotic plaque could go through rapid progression and induce adverse cardiac events. This study aimed to evaluate the impacts of smoking status on clinical outcomes of coronary non-target lesions. METHODS:Consecutive patients with coronary heart disease who underwent two serial coronary ang...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000001024
更新日期:2020-10-05 00:00:00
abstract:OBJECTIVE:To investigate the histologic and histogenetic relationship between various types of rhabdomyosarcomas (RMS) and embryonal skeletal muscle (ESM) for further understanding of the histogenesis, classification and pattern of differentiation of RMS. METHODS:Fifty cases of variant types of RMS and 20 cases of ESM...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract:OBJECTIVE:This review discusses the current status and progress in studies on the roles of hydrogen sulfide (H(2)S) in regulation of neurotoxicity, neuroprotection, and neuromodulator, as well as its therapeutic potential for neurodegenerative disorders. DATA SOURCES:The data used in this review were mainly from Medli...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Epithelial to mesenchymal transition (EMT) is strongly linked with tumor invasion and metastasis, which performs a vital role in carcinogenesis and cancer progression. Emerging evidence suggests that microRNAs (miRNAs) expression are closely associated to EMT by regulating targeted genes. MiR542 has been fou...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000001072
更新日期:2020-10-20 00:00:00
abstract:OBJECTIVE:To study the potential and advantages of internal distraction osteogenesis in mandibular functional reconstruction. METHODS:Three types of mandibular distractors were used in eight patients with various mandibular defects due to tumor or cyst resection. The average age of the patients was 31.5 years old (age...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00