Abstract:
:Multiple myeloma (MM) is the second most common hematologic malignancy characterized by the clonal expansion of plasma cells. Despite continuing advances, novel biomarkers are needed for diagnosis and prognosis of MM. In our study, we characterized the diagnostic and prognostic potential of circulating microRNAs (miRNAs) in MM. Serum miRNA levels were analyzed in 108 newly diagnosed symptomatic MM patients and 56 healthy donors (HDs). Our analysis identified 95 dysregulated miRNAs in newly diagnosed MM patients. Of the 95 dysregulated miRNAs, dysregulation of miR-19a, miR-92a, miR-214-3p, miR-135b-5p, miR-4254, miR-3658 and miR-33b was confirmed by quantitative reverse transcription PCR (RT-qPCR). Receiver operating characteristic analysis revealed that a combination of miR-19a and miR-4254 can distinguish MM from HD with a sensitivity of 91.7% and specificity of 90.5%. Decreased expression of miR-19a was positively correlated with international staging system advancement, del(13q14) and 1q21 amplification. Furthermore, downregulation of miR-19a resulted in significantly decreased progression-free survival (PFS) and overall survival (OS). Our analysis indicated that the poor prognostic correlation of miR-19a expression was independent of genetic abnormalities in MM. Multivariate analysis revealed that miR-19a was a significant predictor of shortened PFS and OS. Interestingly, although miR-19a levels portend a poor prognosis, patients with low miR-19a levels had an improved response to bortezomib compared to those with high miR-19a profile. Patients with downregulated miR-19a experienced a significantly extended survival upon bortezomib-based therapy. These data demonstrate that the expression patterns of serum microRNAs are altered in MM, and miR-19a levels are a valuable prognostic marker to identify high-risk MM.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Hao M,Zang M,Wendlandt E,Xu Y,An G,Gong D,Li F,Qi F,Zhang Y,Yang Y,Zhan F,Qiu Ldoi
10.1002/ijc.29199subject
Has Abstractpub_date
2015-04-15 00:00:00pages
1835-44issue
8eissn
0020-7136issn
1097-0215journal_volume
136pub_type
杂志文章abstract::Neuroblastoma (NBL), one of the most common childhood solid tumors, has a distinct nature in different prognostic subgroups. However, the precise mechanism underlying this phenomenon remains largely unknown. To understand the molecular and genetic bases of neuroblastoma, we have generated its cDNA libraries and identi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20431
更新日期:2004-11-10 00:00:00
abstract::A case-control study of 374 patients with primary epithelial cancers of the oral cavity, oro- and hypopharynx, and larynx is reported, the controls being patients with selected other cancers, matched for age and sex. Of all eligible patients, 93% were interviewed. Increased risks were seen with alcohol consumption and...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910340504
更新日期:1984-11-15 00:00:00
abstract::Several mouse models studying the MLL fusion-induced leukemic transformation showed that a myeloproliferation stage precedes leukemia or occurred as the only phenotype of hematological disorder in mice. We established 6 MLL/AF10(OM-LZ)-immortalized cell lines by retrovirally transducing the fusion gene into bone marro...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24867
更新日期:2010-04-01 00:00:00
abstract::We previously produced evidence that the human mammary-carcinoma cell line 8701-BC expresses several metalloproteinases (MMP-1, -2, -9, and -10) and their tissue inhibitors). In order to obtain a better understanding of the environmental control over gelatinolytic activities, we have tested the enzyme production of 87...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910620622
更新日期:1995-09-15 00:00:00
abstract::The effects of concomitant administration of bombesin and of the diuretic drug amiloride on the development of large and small intestinal tumors induced by azoxymethane (AOM), the incidence of their metastasis to the peritoneum and the labeling index of intestinal adenocarcinomas were investigated in inbred Wistar rat...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910630518
更新日期:1995-11-27 00:00:00
abstract::DNA isolated from blood or bone-marrow samples from 18 patients with acute non-lymphocytic leukemia (ANLL) and 14 patients with acute lymphocytic leukemia (ALL) was analyzed for the presence of mutations in the N-ras gene. Using synthetic oligonucleotide probes we detected mutations in 5 cases of ANLL; 4 GGT----GAT tr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910410112
更新日期:1988-01-15 00:00:00
abstract::Dichlorodiphenyltrichloroethane (p,p'-DDT), an organochlorine pesticide known to have deleterious health effects in humans, has been linked to hepatocellular carcinoma (HCC) in rodents. A recent study has reported that p,p'-DDT and its most persistent metabolite, dichlorodiphenyldichloroethylene (p,p'-DDE), may also b...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27459
更新日期:2012-11-01 00:00:00
abstract::Metastasis to the liver often occurs in patients during the natural course of pancreatic cancer. Using carcinoma cell lines established from 9 such patients, we examined phenotypes of cell lines to search for correlations with their potential to metastasize to the liver. Anti-asialo GMI-treated nude mice were used. PC...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960822)69:4<290::AID-IJC
更新日期:1996-08-22 00:00:00
abstract::In normal somatic cells, the methylation pattern of DNA is stably maintained by DNA (cytosine-5-)-methyltransferase (DNA methyltransferase). Increased expression of DNA methyltransferase has been detected in many types of human cancer and has been thought to play an important role in tumorigenesis. In our study, we de...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990924)83:1<10::aid-ijc3
更新日期:1999-09-24 00:00:00
abstract::Immunostimulatory DNA containing unmethylated cytosine-guanine (CpG) motifs have been successfully used as adjuvants to enhance the immunity of vaccines designed to trigger antitumor T-cell responses. We examined the effect of a CpG oligodeoxynucleotide (CpG ODN) for its ability to potentiate the activity of tumor ant...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24009
更新日期:2009-02-15 00:00:00
abstract::Diabetes is a risk factor for many cancers; chronic hyperglycemia is hypothesized to be, in part, explanatory. We evaluated the association between glycated hemoglobin, a time-integrated glycemia measure, and cancer incidence and mortality in nondiabetic and diabetic men and women. We conducted a prospective study of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27394
更新日期:2012-10-01 00:00:00
abstract::Retinoids are a group of vitamin A analogues that have shown promise as chemopreventive and therapeutic agents in many types of malignancy and have been entered in clinical trials with some successful results. To better understand the mechanism that mediates retinoid action and the anti-proliferative effects, we treat...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970117)70:2<194::aid-ijc
更新日期:1997-01-17 00:00:00
abstract::The putative tumor suppressors RASSF1A and BLU are mapped adjacent to one another on chromosome 3p21.3, a region frequently deleted in lung cancer. These genes are often inactivated by promoter hypermethylation, but the association of this inactivation with clinical features of the disease or with carcinogen exposure ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20714
更新日期:2005-03-20 00:00:00
abstract::To assess the relation between air pollution with airway irritants, including sulfuric acid and lung cancer, a case-control study was performed near an industry producing sulfuric acid and fertilizers in Kedainiai county, central Lithuania. The county had the highest lung cancer rates of the country among men. Between...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10314
更新日期:2002-05-01 00:00:00
abstract::Parvoviruses of rodents are endowed with oncosuppressive properties. In particular, parvoviral infections protect host animals from spontaneous and chemical- or virus-induced tumour initiation in laboratory animals. The present study was undertaken to substantiate the capacity of parvovirus H-1 to inhibit therapeutica...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980209)75:4<584::aid-ijc
更新日期:1998-02-09 00:00:00
abstract::Although tobacco smoking, pan chewing and alcohol drinking are important risk factors for head and neck cancer (HNC), the HNC risks conferred by products available in Nepal for these habits are unknown. We assessed the associations of tobacco smoking, chewing habits, and alcohol drinking with HNC risk in Nepal. A case...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32823
更新日期:2020-08-01 00:00:00
abstract::There are no reliable serologic tumor markers for transitional cell carcinoma (TCC) of the urinary bladder and noninvasive urine investigations are inadequate. We used fluorescent microsatellite analysis (MSA) to detect serum DNA and urine-sediment DNA alterations in patients with bladder cancer and prospectively coll...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1434
更新日期:2001-10-01 00:00:00
abstract::The stability of expression of a membrane antigen on human FME melanoma cells was investigated by means of flow cytometric cell sorting and analysis. The melanoma-associated 250 kd antigen was strongly expressed on all cells, as recognized by binding of the monoclonal antibody 9.2.27. By flow cytometric cell sorting, ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910340413
更新日期:1984-10-15 00:00:00
abstract::The neural crest gives rise to a variety of tissues, including peripheral neurons, Schwann cells, melanocytes and ectomesenchymal cells, which include the smooth-muscle cells of large arteries. Cell lines derived from neuroblastoma (a neural-crest tumor) exhibit at least 2 distinct morphological cell types, a neurobla...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480221
更新日期:1991-05-10 00:00:00
abstract::We report here side population (SP) cells, a cancer stem cell enriched fraction from pancreatic cancer cell line, have enormous superior potential of the epithelial to mesenchymal transition (EMT), invasion, and metastasis. In an isolated SP cell culture, the cells rapidly expressed and up-regulated E-cadherin, an epi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24349
更新日期:2009-06-15 00:00:00
abstract::The aim of this prospective study was to assess the uptake of predictive genetic testing for hereditary non-polyposis colorectal cancer (HNPCC) and its associations with sociodemographic and other factors, and long-term satisfaction with taking the test. The test was offered to all high-risk members (n = 446) of 36 Fi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-01-20 00:00:00
abstract::The effects of DL-alpha-difluoromethylornithine (DFMO), a specific irreversible inhibitor of ornithine decarboxylase, on the growth of experimental mouse B16-F10 melanoma cells were investigated. DFMO (3%) in drinking water was administered to B16-F10 melanoma-bearing mice. At 24 days, B16-F10 melanomas in DFMO-fed mi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390220
更新日期:1987-02-15 00:00:00
abstract::The antitumor activity of highly purified tumor necrosis factor (TNF) was tested against eight kinds of murine tumor and five kinds of human tumor heterotransplanted into nude mice. Mice were treated by intravenous or intratumoral injection of TNF, commencing when the tumors were well established. TNF showed an excell...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910340219
更新日期:1984-08-15 00:00:00
abstract::The tumor-suppressor genes p14(ARF), p16(INK4a) and Tp53 are commonly inactivated in many tumors. We investigated their role in the pathogenesis of 9 bile tract cancer cell lines and 21 primary sporadic extrahepatic bile duct carcinomas. p53 and p16 protein expression was examined by Western blot analysis and immunohi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1639
更新日期:2002-02-01 00:00:00
abstract::Circulating tumor DNA (ctDNA) is a new circulating tumor biomarker which might be used as a prognostic biomarker in a way similar to circulating tumor cells (CTCs). Here, we used the high prevalence of TP53 mutations in triple negative breast cancer (TNBC) to compare ctDNA and CTC detection rates and prognostic value ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29265
更新日期:2015-05-01 00:00:00
abstract::Ferric nitrilotriacetate (Fe-NTA) causes renal proximal tubular necrosis, a consequence of iron ion-mediated free-radical-associated damage, that finally leads to a high incidence of renal adenocarcinoma in male rats and mice. We have investigated the levels of typical hydroxyl radical-induced DNA base modifications i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570122
更新日期:1994-04-01 00:00:00
abstract::The expression of oestrogen (ER), progesterone (PR) and androgen (AR) receptors in female breast cancer was investigated by immunohistochemistry on snap-frozen tissue specimens of a series of 100 breast cancers. For detection of the AR we used a recently developed mouse monoclonal antibody specific for the N-terminal ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520415
更新日期:1992-10-21 00:00:00
abstract::Using antisera for two specific basement membrane (b.m.) antigens such as laminin and collagen type-IV, together with electron microscopy, we have shown that fully antigenic b.m. and morphologically typical basal lamina (b.l.) are associated with normal and transformed cells of the melanocyte lineage in different ways...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910350408
更新日期:1985-04-15 00:00:00
abstract::We have previously reported that losses of genomic material from the long arm of chromosome 18 are frequent in atypical and anaplastic meningiomas but rare in benign meningiomas. In the present study, we have investigated a series of 37 meningiomas for mutation and expression of 4 tumor suppressor genes (MADH2, MADH4,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1219
更新日期:2001-05-15 00:00:00
abstract::The association between fish, ω-3 and ω-6 polyunsaturated fatty acid (PUFA) intake and risk of colorectal cancer (CRC) remains inconclusive. Recent prospective studies suggest that the relationship may vary by gender, subsite and duration of follow-up. We followed 123,529 US adults (76,386 women and 47,143 men) withou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28878
更新日期:2014-11-15 00:00:00