Abstract：
OBJECTIVE:To describe the antimicrobial activity of β-defensin-2 produced in the mammary gland and secreted in human breast milk. METHODS:The peptide production was performed by DNA cloning. β-defensin-2 levels were quantified in 61 colostrum samples and 39 mature milk samples from healthy donors, by an indirect enzyme-linked immunosorbent assay (ELISA). Using halo inhibition assay, this study assessed activity against seven clinical isolates from diarrheal feces of children between 0 and 2 years of age. The activity of β-defensin-2 against three opportunistic pathogens that can cause nosocomial infections was determined by microdilution test. RESULTS:The peptide levels were higher in colostrum (n=61) than in mature milk samples (n=39), as follows: median and range, 8.52 (2.6-16.3) μg/ml versus 0.97 (0.22-3.78), p<0.0001; Mann-Whitney test. The recombinant peptide obtained showed high antimicrobial activity against a broad range of pathogenic bacteria. Its antibacterial activity was demonstrated in a disk containing between 1-4 μg, which produced inhibition zones ranging from 18 to 30 mm against three isolates of Salmonella spp. and four of E. coli. β-defensin-2 showed minimum inhibitory concentrations (MICs) of 0.25 μg/mL and 0.5 μg/mL for S. marcescen and P. aeruginosa, respectively, while a higher MIC (4 μg/mL) was obtained against an isolated of multidrug-resistant strain of A. baumannii. CONCLUSIONS:To the authors' knowledge, this study is the first to report β-defensin-2 levels in Latin American women. The production and the activity of β-defensin-2 in breast milk prove its importance as a defense molecule for intestinal health in pediatric patients.
journal_name
J Pediatr (Rio J)journal_title
Jornal de pediatriaauthors
Baricelli J,Rocafull MA,Vázquez D,Bastidas B,Báez-Ramirez E,Thomas LEdoi
10.1016/j.jped.2014.05.006subject
Has Abstractpub_date
2015-01-01 00:00:00pages
36-43issue
1eissn
0021-7557issn
1678-4782pii
S0021-7557(14)00121-1journal_volume
91pub_type
杂志文章abstract：OBJECTIVE:To evaluate bone mineral density and bone mineral content of skeleton and lumbar spine of pre-pubertal children and to correlate results thus found with sex, bone age, body weight, height and insulin-like growth factor 1 serum level. METHODS:26 boys and 12 girls aged between 7 and 8 years old, students from ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.555
更新日期：1997-07-01 00:00:00
abstract：OBJECTIVE:To review current concepts related to the procedure of tracheal intubation in children. SOURCES:Relevant articles published from 1968 to 2006 were selected from the MEDLINE, LILACS and SciELO databases, using the keywords intubation, tracheal intubation, child, rapid sequence intubation and pediatric airway....
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/JPED.1626
更新日期：2007-05-01 00:00:00
abstract：OBJECTIVE:To observe the intensity of the inflammatory reaction caused by urine on the intestinal wall of rats. METHODS:Experimental model, using 20 Wistar rats divided into two groups. All the animals were submitted to abdominal puncture at the right inferior quadrant, twice daily for five days. In Group I (control g...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.1113
更新日期：2003-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the peak inspiratory pressure and ventilation rate achieved by physicians when using a neonatal self-inflating bag on a lung model. METHODS:Fifteen physicians ventilated full term and preterm infant lung simulators while the outcomes were captured by a ventilation monitor. RESULTS:Median peak pr...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/JPED.1524
更新日期：2006-09-01 00:00:00
abstract：:OBJECTIVE: Aiming a better understanding of the distribution of enteropathogens in Brazil, we have investigated the prevalence of enteropathogens in acute infantile diarrhea in children of low economic level from São José do Rio Preto, SP.METHODS: A prospective study with 196 children under age five, all with diarrhea...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.444
更新日期：1998-07-01 00:00:00
abstract：OBJECTIVE:To contribute with information about the adequate administration of drugs during breastfeeding. SOURCES OF DATA:MEDLINE articles from 1993 to 2004, and Lilacs articles from 1995 to 2004 were selected in Portuguese, Spanish and English, by including the following keywords: breastfeeding, lactation, drugs and ...
journal_title：Jornal de pediatria
pub_type： 杂志文章,评审
doi：10.2223/1246
更新日期：2004-11-01 00:00:00
abstract：OBJECTIVE:To analyze the occurrence of anemia and iron deficiency in children aged 1 to 5 years and the association of these events and retinol deficiency. METHODS:This was an observational analytic cross-sectional study conducted in Vitoria, ES, Brazil, between April and August of 2008, with healthy children aged 1 t...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2014.03.003
更新日期：2014-11-01 00:00:00
abstract：OBJECTIVE:To generate prediction equations for spirometry in 4- to 6-year-old children. METHODS:Forced vital capacity, forced expiratory volume in 0.5s, forced expiratory volume in one second, peak expiratory flow, and forced expiratory flow at 25-75% of the forced vital capacity were assessed in 195 healthy children ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2015.10.005
更新日期：2016-07-01 00:00:00
abstract：:OBJECTIVE: To assess the prevalence of prenatal exposure to cocaine in a sample of newborns using two methods: fluorescence polarization immunoassay and interview with the mother. METHODS: This cross-sectional study was carried out in a university teaching hospital. The population included all live births between Marc...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.277
更新日期：2001-09-01 00:00:00
abstract：OBJECTIVE:to determine, by Doppler-echocardiography, the frequency of cardiac diastolic dysfunction in asymptomatic and clinically stable pediatric patients with vertical infection by the human immunodeficiency virus (HIV), from the cardiovascular viewpoint. METHODS:this was an observational, prospective, and cross-se...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2014.01.004
更新日期：2014-07-01 00:00:00
abstract：:Immediate skin test reactions are the hallmark of atopic diseases. EMLA (eutectic mixture of local anesthetics) is a local anesthetic that reduces the pain and apprehension for skin testing. We studied EMLA effects on the skin prick test with allergen and histamine. Reactions were evaluated by means of wheal and flare...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.613
更新日期：1996-07-01 00:00:00
abstract：OBJECTIVE:To emphasize the importance of precocious diagnosis of extrahepatic biliary atresia and its direct relationship with the surgical re-establishment of the biliary flow before the second month of life. To discuss several complementary methods with the aim of selecting the ones that present better evidence, and ...
journal_title：Jornal de pediatria
pub_type： 杂志文章,评审
doi：
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVE:Assess the maternal socio-demographic factors associated with the frequency of use of child care services by low income families. METHODS:Cross-sectional analysis of 393 children between 12 and 16 months old who participated in a randomized field trial during their first year of life in a program of nutritio...
journal_title：Jornal de pediatria
pub_type： 杂志文章,随机对照试验
doi：10.2223/JPED.1966
更新日期：2010-01-01 00:00:00
abstract：:OBJECTIVE: Describe the hemodynamic response with rebound of pulmonary hypertension after withdrawal of inhaled nitric oxide (NO) in a pediatric patient with acute respiratory distress syndrome (ARDS). METHODS: Case report of a child with ARDS and pulmonary hypertension evaluated through ecocardiografic with dopller, ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.59
更新日期：2000-03-01 00:00:00
abstract：:OBJECTIVE: This paper examines the main causes of mortality among adolescents ages 10 to 19 in the city of Rio de Janeiro with a focus on mortality prevention. METHODS: Mortality data from 1981 to 1995 were taken from the Mortality Information System of the Brazilian Ministry of Health. Age and sex specific mortality ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.325
更新日期：1999-09-01 00:00:00
abstract：OBJECTIVE:to investigate the pulmonary response to exercise of non-morbidly obese adolescents, considering the gender. METHODS:a prospective cross-sectional study was conducted with 92 adolescents (47 obese and 45 eutrophic), divided in four groups according to obesity and gender. Anthropometric parameters, pulmonary ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2013.08.005
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVE:To analyze acute splenic sequestration (ASS) in children with sickle cell anemia diagnosed through a newborn screening program in the state of Minas Gerais, Brazil, and followed up at the hematology center in the city of Belo Horizonte, Minas Gerais, Brazil. METHODS:Retrospective cohort of 255 children with ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/JPED.1885
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVE:Children's differences in moderate-to-vigorous physical activity levels are not at random. This study investigates the relevance of individual- and school-level characteristics in explaining these differences. METHODS:In total, 307 children (154 girls) aged 5-10 years, from 19 Portuguese schools, were sample...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2018.10.013
更新日期：2020-01-01 00:00:00
abstract：:OBJECTIVE: To determine the prevalence of rotavirus in the etiology of acute diarrhea in children from Natal city, RN, Brazil and investigate the existence or not of a seasonal distribution of this pathogen in our environment.METHODS: Fecal samples from 1,903 children (boys and girls) with ages ranging from 1 month to...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.6
更新日期：2000-07-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to investigate what effect the ingestion of sardines, rich in omega-3 series polyunsaturated fatty acids, has on the composition of breastmilk. METHODS:This was a prospective study of 31 nursing mothers under observation at the Hospital Guilherme Alvaro. Each was given 2 kg of ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/JPED.1439
更新日期：2006-01-01 00:00:00
abstract：:OBJECTIVES: To identify rubella prevalence in different ages and population groups and rubella susceptibility of pregnant and postpartum women according to age, number of children and spontaneous abortion. METHODS: Cross-sectional study of sero-survey type. Children and students were selected in day-care centers and s...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/jped.470
更新日期：1998-11-01 00:00:00
abstract：OBJECTIVE:To describe the association between electronic devices in the bedroom with sedentary time and physical activity, both assessed by accelerometry, in addition to body mass index in children from São Caetano do Sul. METHODS:The sample consisted of 441 children. The presence of electronic equipment (television, ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2015.01.009
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVE:To assess the prevalence of metabolic syndrome-like symptoms in a population of preterm infants with very low birth weight (<1500g) at 2 years of corrected age and identify the occurrence of associated risk factors. METHODS:Cross-sectional study during a five-year period, including preterm infants born with ...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2018.02.009
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVE:To investigate the association between postpartum depression and the occurrence of exclusive breastfeeding. METHOD:This is a cross-sectional study conducted in the states of the Northeast region, during the vaccination campaign in 2010. The sample consisted of 2583 mother-child pairs, with children aged from...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.1016/j.jped.2016.08.005
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:To compare two groups of school age girls from different social strata were compared in terms of their perception and knowledge about breastfeeding. METHODS:A structured questionnaire was applied to all 346 4th to 8th grade girls attending two schools, a private one, whose students belonged to the upper clas...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVE:To verify the concurrent validity and interobserver reliability of the Alberta Infant Motor Scale (AIMS) in premature infants followed-up at the outpatient clinic of Instituto Fernandes Figueira, Fundação Oswaldo Cruz (IFF/Fiocruz), in Rio de Janeiro, Brazil. METHODS:A total of 88 premature infants were enro...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/JPED.1836
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:to assess the epidemiological and genetic factors associated with severity of acute viral bronchiolitis (AVB) by respiratory syncytial virus (RSV). DATA SOURCE:the key words "bronchiolitis", "risk factor", "genetics" and "respiratory syncytial virus", and all combinations among them were used to perform a se...
journal_title：Jornal de pediatria
pub_type： 杂志文章,评审
doi：10.1016/j.jped.2013.02.022
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the prevalence of intestinal parasitoses in Native Brazilian children from 2 to 9 years old. METHODS:A search for ova and parasites was conducted in the stools of children between 2 to 9 years old living in six indigenous villages located in the Middle and Lower Xingu River, to wit: Pavuru, Moygu...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/JPED.2036
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To investigate risk factors for anemia in infants assisted by public health services. METHODS:In a cross-sectional study carried out in Viçosa, state of Minas Gerais, Brazil, 205 children from 6 to 12 months were evaluated. Socioeconomic, environmental and biological data were collected, as well as informati...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：10.2223/JPED.1603
更新日期：2007-03-01 00:00:00
abstract：OBJECTIVE:To determine the thyroid stimulating hormone (TSH) secretion in children with Down syndrome (DS), who do not present clinical and laboratory evidence of classical hypothyroidism and concomitant undetectable antibodies. METHODS:Fourteen children with DS with a mean age of 3.4 (+/- 1.8) years were studied. Pat...
journal_title：Jornal de pediatria
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
