Abstract:
:Methylthiophanate is one of the widely used fungicides to control important fungal diseases of crops. The aim of this study was to elucidate the short-term hematoxicity and genotoxicity effects of methylthiophanate administered by intraperitoneal way at three doses (300, 500 and 700 mg/kg of body weight) after 24, 48 and 72 h. Our results showed, 24 h after methylthiophanate injection, a hematological perturbation such as red blood cells (p < 0.05, p < 0.05 and p < 0.01) and hemoglobin content (p < 0.05), respectively, and a noticeable genotoxic effect in WBC evidenced by a significant increase in the frequency of the micronuclei and a decrease in cell viability. An increase in erythrocyte osmotic fragility was also noted after 24 and 48 h of methylthiophanate treatment at graded doses. A significant increase in hydrogen peroxide, advanced oxidation of protein products and malondialdehyde levels, in erythrocytes of methylthiophanate-treated rats with 300, 500 and 700 mg/kg of body weight, was also observed after 24 h of treatment (p < 0.05, p < 0.01 and p < 0.001, respectively), suggesting the implication of oxidative stress in its toxicity. Antioxidants activities of superoxide dismutase and glutathione peroxidase in erythrocytes significantly increased (p < 0.001) 24 h after the highest dose injected. While all these parameters were improved after 72 h of methylthiophanate injection (300, 500 and 700 mg/kg body weight). In conclusion, these data showed that the exposure of adult rats to methylthiophanate resulted in oxidative stress leading to hematotoxicity and the impairment of defence system, confirming the pro-oxidant and genotoxic effects of this fungicide.
journal_name
Toxicol Mech Methodsjournal_title
Toxicology mechanisms and methodsauthors
Ben Amara I,Ben Saad H,Cherif B,Elwej A,Lassoued S,Kallel C,Zeghal Ndoi
10.3109/15376516.2014.961217subject
Has Abstractpub_date
2014-12-01 00:00:00pages
679-87issue
9eissn
1537-6516issn
1537-6524journal_volume
24pub_type
杂志文章abstract::Color and geometric characteristics of stained areas in histochemical slides are among the features pathologists assess to evaluate the severity of lesions. In this research, image processing techniques were used to perform objective quantification of these characteristics in images of H&E-stained spleen tissues. A se...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376520490434638
更新日期:2004-01-01 00:00:00
abstract::This report explores the direct counting of "hair-like" struc-tures specific for Gram-positive bacteria. Indications show that these structures are intact after removal from the cell and are sufficiently different from species to species of bacteria to give an indication of bacteria type if not actual identification. ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510600910477
更新日期:2006-01-01 00:00:00
abstract::Up to now, the ability of target cells to activate protein kinase C (PKC) and protein kinase D (PKD) (which is often a downstream target of PKC) has not been examined in natural killer (NK) lymphocytes. Here we examined whether exposure of human NK cells to lysis sensitive tumor cells activated PKC and PKD. The result...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1070226
更新日期:2015-01-01 00:00:00
abstract:AIM:Nicotine exerts a number of physiological effects. The purpose of this study was to determine the effects of nicotine on thioacetamide (TAA)-induced liver fibrosis in mice. MATERIALS AND METHODS:For in vivo experiments, hepatic fibrosis was induced by TAA (0.25 g/kg, i.p.) three times a week for 6 weeks. Mice of T...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2017.1323256
更新日期:2017-09-01 00:00:00
abstract::The specific properties of silver nanoparticles (AgNPs), such as antimicrobial activity and electrical conductivity, allow them to be used in many fields. However, their expanding application is also raising health, environmental and safety concerns. Previous in vivo AgNP toxicity studies have indicated a gender-diffe...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2013.780196
更新日期:2013-07-01 00:00:00
abstract::Limited information is available regarding systemic changes in mammals associated with exposures to petroleum/hydrocarbon fuels. In this study, systemic toxicity of JP-8 jet fuel was observed in a rat inhalation model at different JP-8 fuel vapor concentrations (250, 500, or 1000 mg/m(3), for 91 days). Gel electrophor...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376511003681009
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND:Arsenic has been shown to cause various diseases (such as blackfoot disease, cardiovascular diseases, bladder cancer and skin cancer) in many areas of the world. However, the effects of arsenic on cardiac rhythm functions still lack investigation. METHODS:In this study, different concentrations of arsenic w...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2018.1440679
更新日期:2018-07-01 00:00:00
abstract::ABSTRACT Although copper is an essential metal and cadmium is an environmental pollutant, both are toxic when present in excess. Metallothionein and glutathione are two of the key components that participate in detoxification of copper and cadmium. In the present study the role of glutathione in resistance to copper a...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510601091392
更新日期:2007-01-01 00:00:00
abstract::Misuse of various chemicals, such as chemical warfare agents, industrial chemicals or pesticides during warfare or terrorists attacks requires adequate protection. Thus, development and evaluation of novel decontamination dispositives and techniques are needed. In this study, in vitro permeation and decontamination of...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.686535
更新日期:2012-09-01 00:00:00
abstract::The aim of this study was to evaluate the effects of different doses of an adenosine A(1) selective agonist, phenylisopropyl adenosine (PIA), on metamidophos-induced cholinergic symptoms, mortality, diaphragm muscle necrosis, and brain oxidative stress. A LD(50) dose of metamidophos (20 mg/kg body weight, p.o.) was fo...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510802355141
更新日期:2009-02-01 00:00:00
abstract::A suboptimal in utero environment can have detrimental effects on the pregnancy and long-term adverse "programing" effects on the offspring. Aflatoxin B1 is one of the potent reproductive toxicants and currently detected in both milk and tissues. This article focuses on the effects of prenatal exposure to graded doses...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2016.1151967
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Gentamicin (GM) is used against bacterial infections. The aim of our investigation was to evaluate the role of inflammation and also oxidative damage in nephrotoxic potential of GM and protective effects of Nasturtium officinale (watercress) against GM-induced nephrotoxicity in Wistar rats. MATE...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2016.1258748
更新日期:2017-02-01 00:00:00
abstract::The epidemiological association between black cherry trees and mare reproductive loss syndrome has focused attention on cyanide and environmental cyanogens. This article describes the toxicokinetics of cyanide in horses and the relationships between blood cyanide concentrations and potentially adverse responses to cya...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510309832
更新日期:2003-01-01 00:00:00
abstract::Silver and gold nanoparticles (Ag-AuNPs) are currently some of the most manufactured nanomaterials. Accordingly, the hazards associated with human exposure to Ag-AuNPs should be investigated to facilitate the risk assessment process. In particular, because pulmonary exposure to Ag-AuNPs occurs during handling of these...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1025347
更新日期:2015-01-01 00:00:00
abstract::In this study, 162 samples were analysed for monomer styrene content with using high performance liquid chromatography (HPLC) method in hot tea, milk, cocoa milk. The monomer styrene content, expressed in microg/l of drink and the level of migration of styrene monomer were varied from 0.61 to 8.15 for hot tea, from 0....
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510802510299
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:The anatomical and histological parameters of the human ventricle depend on many factors including age and sex. Myocyte volume and electric capacitance are significant physiological parameters of left ventricle cardiomyocyte mathematical models. They allow the assessment of inter-individual variability durin...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2011.585477
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The objective of the present study is to evaluate the antihyperlipidemic effect of Chlorella pyrenoidosa in diabetic rats exposed to cadmium (Cd). MATERIALS AND METHODS:Group 1 and 2 rats were treated as control and C. pyrenoidosa control. Group 3 and 4 rats were given single injection of streptozotocin (40 ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2012.714007
更新日期:2012-10-01 00:00:00
abstract::The present study investigated the protective effect of hesperidin on carbohydrate metabolizing enzymes in streptozotocin-induced diabetic rats. Hesperidin was administered to streptozotocin-induced (40 mg/kg b.w.) diabetic rats at different dosages of (25, 50, 100 mg/kg b.w.) respectively for 30 days to evaluate its ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2019.1646370
更新日期:2019-11-01 00:00:00
abstract::Angiogenesis, disturbance in redox homeostasis, and deregulated redox signaling are considered as common hallmarks of cancer progression and chemo resistance. In this context, PERK (protein kinase PKR-like ER kinase) branch of the unfolded protein response (UPR), an adaptive mechanism triggered by endoplasmic reticulu...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2020.1727596
更新日期:2020-05-01 00:00:00
abstract::Oxidative stress in biological systems can result in radical-induced lipid peroxidation (LPO), which can lead to the production of secondary reactive by-products such as 4-hydroxy-2-nonenal (HNE), malondialdehyde (MDA), acrolein, and acetaldehyde. These deleterious compounds are known to react with and concomitantly m...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510903104224
更新日期:2009-09-01 00:00:00
abstract::ABSTRACT The ideal toxicity biomarker is composed of the properties of prediction (is detected prior to traditional pathological signs of injury), accuracy (high sensitivity and specificity), and mechanistic relationships to the endpoint measured (biological relevance). Gene expression-based toxicity biomarkers ("sign...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510701857072
更新日期:2008-01-01 00:00:00
abstract::This article described an improved brine shrimp larvae lethality microwell test method. A simply designed connecting vessel with alternative photoperiod was used to culture and collect high yield of active Artemia parthenogenetica nauplii for brine shrimp larvae lethality microwell test. Using this method, pure A. par...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2011.583297
更新日期:2012-01-01 00:00:00
abstract::The influence of experimental conditions on gradient high-performance liquid chromatography/diode array detector separation and identification of 13 psychotropic drugs belonging to two groups--phenothiazines and tricyclic antidepressants--was examined. The main interaction effects of three experimental factors were de...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510309842
更新日期:2003-01-01 00:00:00
abstract::Dioxins and dioxin-like compounds (DLCs) are the ones with poor water solubility and low volatility, resistant to physical, chemical and biological processes, persistent in the environment even under extreme conditions. Due to lipophilic nature, they get adhered to the fatty material and concentrate through biomagnifi...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2016.1273423
更新日期:2017-02-01 00:00:00
abstract::The brain is a well-organized, yet highly complex, organ in the mammalian system. Most investigators use the whole brain, instead of a selected brain region(s), for biochemical analytes as toxicological endpoints. As a result, the obtained data is often of limited value, since their significance is compromised due to ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376520490429175
更新日期:2004-01-01 00:00:00
abstract::Multi-walled carbon nanotubes (MWCNTs) have industrial applications in the nanotechnology field. The physico-chemical properties of MWCNTs vary greatly depending on MWCNT manufacture and application. It has been pointed out that their needle shape and high durability are important factors that determine the biopersist...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376516.2020.1761920
更新日期:2020-09-01 00:00:00
abstract::This study was to evaluate the protective effects of Danshensu on liver injury induced by omethoate in Sprague Dawley rats. The acute omethoate poisoning model was established by administrating subcutaneously with omethoate at a single dose of 60 mg/kg. Danshensu treatment markedly inhibited the increases of aspartate...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2010.514963
更新日期:2010-10-01 00:00:00
abstract::Some environmental pollutants derived from industrial processes have been suggested to be responsible for neurological impairment in children, especially in heavily polluted areas. Since these compounds are usually activators of aryl hydrocarbon receptor (AhR), it would be important to better understand the molecular ...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2015.1070227
更新日期:2015-01-01 00:00:00
abstract::Due to their exceptional properties, gold nanoparticles (AuNPs) have shown promising medical and technological applications in the treatment of cancer and the development of antimicrobial packaging and time-temperature indicators in the food sector. However, little is known about their cytotoxicity when they come into...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.3109/15376516.2013.869783
更新日期:2014-03-01 00:00:00
abstract::ABSTRACT Oxidative damage to cellular constituents is one of the major mechanisms of alcoholic liver injury, and administration of antioxidants ameliorates alcoholic liver disease. The present study investigated the influence of (-) epigallocatechin gallate (EGCG), a major polyphenol component of green tea, on oxidant...
journal_title:Toxicology mechanisms and methods
pub_type: 杂志文章
doi:10.1080/15376510701884985
更新日期:2008-01-01 00:00:00