Abstract：
:The influenza A virus RNA polymerase cleaves the 5' end of host pre-mRNAs and uses the capped RNA fragments as primers for viral mRNA synthesis. We performed deep sequencing of the 5' ends of viral mRNAs from all genome segments transcribed in both human (A549) and mouse (M-1) cells infected with the influenza A/HongKong/1/1968 (H3N2) virus. In addition to information on RNA motifs present, our results indicate that the host primers are divergent between the viral transcripts. We observed differences in length distributions, nucleotide motifs and the identity of the host primers between the viral mRNAs. Mapping the reads to known transcription start sites indicates that the virus targets the most abundant host mRNAs, which is likely caused by the higher expression of these genes. Our findings suggest negligible competition amongst RdRp:vRNA complexes for individual host mRNA templates during cap-snatching and provide a better understanding of the molecular mechanism governing the first step of transcription of this influenza strain.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sikora D,Rocheleau L,Brown EG,Pelchat Mdoi
10.1038/srep06181subject
Has Abstractpub_date
2014-08-26 00:00:00pages
6181issn
2045-2322pii
srep06181journal_volume
4pub_type
杂志文章abstract：:MicroRNAs are potent regulators of gene expression that have been widely implicated in reproduction and embryo development. Recent studies have demonstrated that miR-21, a microRNA extensively studied in the context of disease, is important in multiple facets of reproductive biology including folliculogenesis, ovulati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29874-w
更新日期：2018-08-01 00:00:00
abstract：:It has been recently ascribed to several inflammatory cytokines (i.e. TGF-β3, TNF-α, and IL-1) a functional role in regulating Sertoli cell blood-testis barrier (BTB) dynamics. In the testis, IL-6 inhibits meiotic DNA synthesis during the seminiferous epithelium cycle, reduces sperm motility and influences the secreti...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2014-03-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-12-03 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-07-26 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2014-12-05 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2014-03-10 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-12-08 00:00:00
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